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We narrowed to 511 results for: plasmid dna

Showing: 321 - 340 of 511 results

  1. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles...barcoding Addgene’s own AAV6 and AAV11 packaging plasmids Mapping anterograde synaptic connections with ...experiments and see the difference! Find mGold2 plasmids here! Lee, J., Lai, S., et al. (2025). Bright...Effector nucleases are shown bound to the target DNA, recruited by either the ωRNA (TnpB) or a gRNA (Cas9... to the broad host range of TRV. Find TRV-TnpB plasmids here! Weiss, T., et al. (2025). Viral delivery...j.stem.2025.01.012.  New AAV6 and AAV11 packaging plasmids from Addgene By Meghan Rego Attention AAV users...delivery toolkit, Addgene now offers packaging plasmids for AAV6 and AAV11! Why these serotypes? AAV6 ...

  2. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...Hutchinson Cancer Research Center developed a pooled plasmid library encompassing all possible amino acid mutations...anti-SARS-CoV-2 antibodies. In the library, each plasmid encodes the mutated spike protein and a barcode...head over to our CRISPR Plasmids and Resources page.  A new system uses light to deactivate photocleavable... accumulation of indels to measure time. These plasmids with different promoters driving expression of...

  3. DNA Quantification

    Type
    Protocol
    ...Recovering Plasmid DNA from Bacterial Culture Restriction Digest of Plasmid DNA Background Information During...your DNA sample concentration and purity using Addgene’s DNA Quantification Protocol. Protocols...spectrophotometer using the solution the DNA is resuspended in, but with no DNA added. 'Blanking' measures the ... Protocols DNA Quantification...Quantification DNA Quantification You may also like... Inoculating a Liquid Bacterial Culture Recovering...important to get a quick and accurate reading of DNA concentration and purity. This can be done with a... maximally absorbs. In the case of nucleic acid (DNA and RNA), the maximal absorbance is at 260nm. Protein...

  4. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...consisted of three plasmids: the packaging, envelope, and transfer plasmids. The packaging plasmid carried a mutated... packaging and envelope plasmids are two of Addgene’s most requested plasmids! Lentiviral particles with...Many CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015: ...itself due to a few missing proteins. The envelope plasmid contained a viral envelope to dictate the tropism...the vector could infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 ...genome integration. After co-transfection of these plasmids, 293T cells released transgene-containing lentiviral...recombination between the transfer and packaging plasmids does exist. To further reduce this possibility...

  5. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...stocks of their plasmids. This way, when you want to make more plasmid DNA, the plasmid will already be... for long-term storage of plasmids. Although you can store your plasmid DNA at -20°C, many labs also create... Storage of Plasmids Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids You may also... like... Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture Background Information...able to start an overnight culture for plasmid DNA prep the following day. Tips and FAQ The optimal concentration...glycerol stock the same time you prepare your plasmid DNA. In the morning, when you retrieve your liquid... and growth temperature, can be found on your plasmid's information page. The next day you will be able...

  6. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology Plasmids ...uses non-homologous end joining (NHEJ) to perform DNA double strand break repair instead of homologous ...the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening with the...

  7. CRISPR Guide

    Type
    Collection
    ...purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA. The ease of generating...Browse Plasmids: RNA Targeting , RNA Editing Degrading DNA with Cas3 Rather than initiating DNA cleavage...-stranded DNA plasmid. Small ssDNA oligos often work well for small edits like a single-point mutation...homology with the target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence...will begin to anneal to the target DNA. If the seed and target DNA sequences match, the gRNA will continue...strands of the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...of target DNA. Cas9n increases target specificity. Since two nickases targeting opposite DNA strands are...

  8. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ... Some researchers have used fluorescently tagged DNA-binding proteins to label certain loci, but this ...24360272. PubMed Central PMCID: PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui...25713381. PubMed Central PMCID: PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui...27088723. PubMed Central PMCID: PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira...26344044. PubMed Central PMCID: PMC4666719. Find plasmids from this publication at Addgene. Additional ...Resources on Asddgene.org Find Fluorescent Protein Plasmids Find Fluorescent Protein Kits Find Empty Backbones...

  9. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...interaction due to mismatches between the guide and the DNA target. CFD scores are then modified (“transformed...sequence, one by one, from the PAM outwards so that the DNA sequence is altered but the protein sequence they...software Resources on Addgene.org Find CRISPR plasmids and resources Download the CRISPR eBook Read our...

  10. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...reconstituted after integration; and a sequence of stuffer DNA after the gRNA target site. Donor B contains: 400...Since this approach is dependent on double-strand DNA break repair, if non-homologous end joining (NHEJ...2013) Oversized AAV Transductifon Is Mediated via a DNA-PKcs-independent, Rad51C-dependent Repair Pathway...therapy Learn about the parts of an AAV transfer plasmid Resources on Addgene.org: Check out the Addgene...

  11. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...You may also like... Plasmid Cloning by PCR Restriction Digest of Plasmid DNA DNA Ligation Background ...stranded DNA template strands. The DNA polymerase is also stable enough to now bind to the primer DNA sequence...which would denature DNA polymerases from other species. In addition to the template DNA and the Taq polymerase...several micrograms of target DNA from just a few nanograms of template DNA through several cycles of denaturation...this step enables the DNA polymerase to synthesize and elongate the new target DNA strand accurately and... are using the same template DNA for all your reactions, the Template DNA can be added to the master mix...the number of nanomoles of DNA times 10. For example, if your lyophilized DNA is 38.5nm, add 385µl of water...

  12. Lentivirus Production

    Type
    Protocol
    ...Transfection grade DNAs: Lentiviral Packaging plasmid(s), Envelope plasmid, and Transfer plasmid encoding your...therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, ...transfection plasmids into OptiPro SFM: Reagent Amount per 10 cm dish* Packaging Plasmid ( psPAX2 ) 1.3...1.3 pmol Envelope Plasmid ( pMD2.G ) 0.72 pmol Transfer Plasmid* 1.64 pmol OptiPro SFM to total volume...volume 500 μL *Plasmid concentrations and ratios should be optimized for each transfer plasmid. Pro-Tip Endotoxins...dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would be 83.4 μL of 1 mg/mL PEI in 416.6 μL of OptiPro...the diluted DNA mixture. Add the diluted PEI dropwise while gently flicking the diluted DNA tube. Incubate...

  13. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter... purify a region of genomic DNA and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In...Ampicillin none, need Cas9 plasmid Qi Do you have suggestions for other plasmids that should be added to ...

  14. Protocol - How to Streak a Plate

    Type
    Protocol
    ...for DNA purification will minimize the chance of having a mixture of plasmids in your purified DNA. This...Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture Introduction If you have... stab culture of bacteria and need to purify plasmid DNA from it, you will want to isolate an individual...single colonies, you can proceed to Recovering Plasmid DNA or use the individual colonies for other experiments...antibiotic. Label the bottom of the plate with the plasmid name and the date. It is also a good idea to add...overnight (12-18 hours) at 37 °C. Pro-Tip Some plasmids or bacteria need to be grown at 30 °C instead ... 37 °C. This is often true for large unstable plasmids, which sometimes recombine at 37 °C. Be sure to...

  15. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...taken up the plasmid. This can serve as an alternative to antibiotic selection. Plasmids of this type .... Scientists can easily utilize GFP-containing plasmids as a means to many functional ends. We’ve listed...each protein or domain being tested. Find FRET plasmids here. Split EGFP: An alternative to FRET, split... marking/selection: Expression constructs like plasmids often include GFP as a marker to help identify...nude mice in cancer studies, act as a reporter for DNA double strand break repair, and to label pathogenic...techniques in fluorescent microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide... plasmids 101...

  17. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...sequence in pEcgRNA. Next both plasmids are transformed in E. coli with the donor DNA for editing to take place...beneficial for plasmid clearance. It is more convenient to update the spacer(20nt) on pEcgRNA plasmid as only.... pCutamp cures the plasmids by targeting the ampR promoter in all three plasmids. pCutamp is then removed... 13 plasmids with 14 rounds of transformation in 30 days. Figure 2: (A) MUCICAT plasmids for ... original pCas/pTargetF system consists of one plasmid that expresses the S. pyogenes Cas9, a temperature-sensitive...the pTargetF construct and then introduce both plasmids into the cell. The edit in the chromosome can ...the pMB1 replicon, resulting in cutting of the plasmid. At this point, subsequent introductions of pTargetF...

  18. Video Library

    Type
    Protocol
    ...Storing and Handling Addgene Plasmids What to do after you receive your plasmids from Addgene Instructions...Protocol Purifying DNA from an Agarose Gel Gel purification allows you to isolate and purify DNA fragments based...electrophoresis, you can cut DNA bands out of the agarose gel and purify the DNA samples. This is a commonly...Protocol Diagnostic Restriction Digest Verify a plasmid using restriction enzymes Diagnostic Restriction...Instructions for Storing and Handling Plasmids Genomic Deletions with CRISPR Generate genomic deletions in mammalian...Transformations We walk you through a standard plasmid transformation protocol and offer some tips and...the standard laboratory procedure for separating DNA by size for visualization and purification. Agarose...

  19. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...the challenges of DNA distribution to ensure scientists everywhere can access plasmids despite the evolving... that is something to recognize! Distributing plasmids, viral service, and more For the first time ...to worry about succeeding at our core mission. Plasmid distribution is amazingly still growing at >9% ...Addgene Resources on the Addgene website Find plasmids for your research  Learn about our viral service...

  20. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function...specific base editor that can alter DNA bases without inducing a DNA break. Base editors convert C->T (...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter... purify a region of genomic DNA and its associated proteins, RNA, and DNA. The enCHIP system uses an anti-FLAG...variety of Cas-containing plasmids. Do you have suggestions for other plasmids that should be added to ...
Showing: 321 - 340 of 511 results