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We narrowed to 416 results for: plasmids 101

Showing: 321 - 340 of 416 results

  1. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...methionine (i.e. the plasmid complements the deletion). Find the Plasmids Here Plasmid features enable multiple...the overexpression plasmids. Finally, students determine if the presence of the plasmid corrects any known...diverged. Tips on using the Pathways Over Time plasmids The plasmids are used as part of a semester-long research...less-well studied organism into overexpression plasmids. Students next transform deletion strains from...phenotypic abnormalities due to the deletion (i.e. the plasmid complements the deletion). If the phenotype is ...cerevisiae met deletion strains with overexpression plasmids carrying MET/Met genes from either S. pombe or.... Students then determine if expression of the plasmid-encoded MET gene allows the transformed strain ...

  2. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    Blog Post
    ...CRISPR-Cas13. Science 358:1019–1027 . https://doi.org/10.1126/science.aaq0180 Find plasmids from this paper at...163:759–771 . https://doi.org/10.1016/j.cell.2015.09.038 Find plasmids from this publication at Addgene...284 . https://doi.org/10.1038/nature24049 Find plasmids from this paper at Addgene. Amrani N, Gao XD,...863–868 . https://doi.org/10.1038/nbt.3609 Find plasmids from this publication at Addgene. Kim E, Koo ...8: . https://doi.org/10.1038/ncomms14500 Find plasmids from this publication at Addgene. Kleinstiver...485 . https://doi.org/10.1038/nature14592 Find plasmids from this publication at Addgene. Kleinstiver...869–874 . https://doi.org/10.1038/nbt.3620 Find plasmids from this publication at Addgene. Hu JH, Miller...

  3. Hot Plasmids - February 2022

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    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'...Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system is a cost-effective alternative... ladders. Image from Santilli, et al. 2021 Plasmids are used to express 10, 15, 20, 30, 40, 50, 60...kD proteins or 20, 40, 60, and 80 kD proteins. Plasmids containing 150 or 250 kD proteins are also available..., et al. Molecular Cell. 2021. https://doi.org/10.1016/j.molcel.2021.08.008. ...

  4. Lentiviral Vector Uses and Overview

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    Blog Post
    ...consisted of three plasmids: the packaging, envelope, and transfer plasmids. The packaging plasmid carried a mutated... packaging and envelope plasmids are two of Addgene’s most requested plasmids! Lentiviral particles with...Many CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015: ...itself due to a few missing proteins. The envelope plasmid contained a viral envelope to dictate the tropism...the vector could infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 ...genome integration. After co-transfection of these plasmids, 293T cells released transgene-containing lentiviral...recombination between the transfer and packaging plasmids does exist. To further reduce this possibility...

  5. Adenoviral Vector Production and Troubleshooting

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    Blog Post
    ...down into 5 key steps: Construction of the rAdV plasmid (~1 week): The AdEasyTM system is the most popular...adenoviral vector constructs. It consists of 2 plasmids: a shuttle vector (in which the transgene of interest...how to generate the final recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide...2.*Pro-Tip*: once the correct recombinant pAdV plasmid is identified, re-transform into a bacterial strain...recombination for amplification. The final purified plasmid stock should be analyzed by endonuclease restriction...-S). HEK293 cells are transfected with the AdV plasmid construct from step #1 and allowed to stay in culture...transductions Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page  ...

  6. Hot Plasmids and Viral Preps - July 2021

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    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available...EasyTag workflow combines thoughtfully designed plasmid components with Fluorescence Activated Cell Sorting...available from Addgene, head over to our CRISPR Plasmids and Resources page. Here are some highlights from the...Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use viral preps....    Sun, et al. bioRxiv. 2021. https://doi.org/10.1101/2020.05.04.076067    Novel fluorescent enzyme ...

  7. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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    Blog Post
    ...Addgene.org Find plasmids in Addgene's COVID-19 collection Find SARS-COV-2 pseudotype virus plasmids from Addgene...variants. “We created a series of spike expressing plasmids which encode spikes found on all of the different...response. The Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing COVID-19 research...research. “I hope that by sharing the plasmids other scientists would be able to expand upon the work and...survival. Cell 184:476-488.e11. https://doi.org/10.1016/j.cell.2020.12.015 Garcia-Beltran WF, Lam EC, St...immunity. Cell 184:2372-2383.e9. https://doi.org/10.1016/j.cell.2021.03.013 Additional resources on the ...

  8. Summer SciComm: Preprints

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    Blog Post
    ...deposits of plasmids that have been described in a preprint. In fact, over 1,500 plasmids in our catalog...Depositing plasmids that were used in a preprint provides all the same benefits as sharing those plasmids alongside...can include the Addgene ID for their deposited plasmids in the final journal publication or an updated...survey of bioRxiv authors. bioRxiv. https://doi.org/10.1101/2021.09.07.459259 Sarabipour S, Debat HJ, Emmott...preprint server for biology. bioRxiv. https://doi.org/10.1101/833400  Stern BM, O’Shea EK (2019) A proposal for...

  9. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    Blog Post
    ...We've developed a new plasmid toolkit, the Broad Institute Target Accelerator Plasmid Collection, that we...dx.doi.org/10.1016/j.coisb.2017.02.002. We hope that by releasing these new and exciting plasmid collections...phenotypes. Broad Institute Target Accelerator Plasmid Collections To overcome this first challenge, we...developed the Broad Institute Target Accelerator Plasmid Collections, now widely available to the scientific...in our recent publications, we used the cancer plasmid collections listed above to demonstrate how gene...patients. We hope you find these new cancer variant plasmid collections useful for your studies. Many thanks... Addgene.org Fine More Kits at Addgene Browse Plasmids from the Broad Institute ...

  10. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post
    ... involving two-plasmid or single-plasmid systems (Vo et al., 2020). In the two-plasmid system, an effector...group’s initial report of VchINT, three separate plasmids and multiple promoters were used to express the...effector plasmid (pEffector) expresses all seven required genes and the CRISPR RNA under the same promoter...promoter, and a donor plasmid (pDonor) encodes the mini-transposon. pEffector and pDonor can also be combined...combined in single-plasmid INTEGRATE (pSPIN), which offer simple transformations for smaller cargos, and ...and the ability to easily cure cells of the plasmid after integration. Figure 2: (A) Streamlined ...insertions with Type I-F INTEGRATE using a single plasmid containing a multi-spacer CRISPR array. Workflow...

  11. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post
    ... tube is mislabeled or someone grabs the wrong plasmid? What if bacteria snuck into the prep during the... human error, fragments of DNA from the helper plasmids or the cell genome can be packaged inside the ...) are aligned to the reference sequence of the plasmid used to create the viral prep. In a clean viral...bacterial genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005, Wright et al., 2011). In...collection database, and it may also be aligned to plasmids in our inventory containing the suspected contaminating... the best match for the contaminant is Addgene plasmid #44362. Method validation, limitations, and future...predetermined seed sequences unique to the capsid plasmid. Our recombination software looks for and quantifies...

  12. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Human whole genome...attached fluorescent protein to the DNA. They created plasmids for purification of tTALE-eGFP and tTALE-mCherry...digestion of single molecules in real-time. Find plasmids expressing tTALE-FP Shin et al., Sci. Rep. 2019...Isaacman-Beck et al., bioRxiv 2019. https://doi.org/10.1101/788679 A destabilized GFP reporter for calcineurin...

  13. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...editing. How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair template...of Addgene plasmid options for templates - see the example at right using blue flame plasmid pmScarlet_...desired insert (e.g., mScarlet).   Plasmid ID Plasmid Name Tags 69090 pMJ915 MBP (N ...out our CRISPR Guide Find Plasmids for Cas9 Purification Find CRISPR Plasmids for Mammalian Expression ...pre-CRISPR was very arduous. First, you’d construct a plasmid with your desired changes, long homology arms and...template! If you have a plasmid with your desired insertion feature (e.g., a fluorescent protein), you can...described in Figure 2.  You can also use Addgene plasmids to purify Cas9 protein. The table below contains...

  14. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    .... citrea scarless editing plasmids Sheng Yang’s lab describes a two-plasmid system that combines recombineering...engineering functions in bacteria, but have no fear - plasmids from Addgene depositors are making it easier than... system available from Addgene consists of two plasmids: pCas9: carries Cas9 and chloramphenicol resistance...gRNA and a backbone-targeting gRNA cassette for plasmid curing through "self-destruction" after induction...editing takes two days, and the pTargetF and pCas plasmids can be subsequently cured from the bacteria. Although...but has many other advantages including shorter plasmid curing time. pCRISPomyces for editing in Streptomyces...inserted using BbsI and Golden Gate Assembly. Either plasmid can be also linearized with XbaI to insert extra...

  15. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. Pubmed. Find the plasmids from this publication at...):635-46. doi: 10.1016/j.cell.2014.09.039. Epub 2014 Oct 9. Pubmed. Find the plasmids from this publication....1420024112. Epub 2015 Feb 23. Pubmed. Find the plasmids from this publication at Addgene. Dynamic imaging...

  16. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post
    ...enChIP Plasmids? All Hodaka Fujii lab plasmids Sign up to receive email alerts when new plasmids from ... shopping for a wide range of plasmids. The merit of depositing plasmids to Addgene has been spectacular... researchers all over the world have requested plasmids for enChIP through Addgene. Retroviral Expression...user-friendly and precise. We will deposit the plasmids of these systems to Addgene in the near future...Community Addgene: In 2012 your lab first deposited plasmids with Addgene. In 2014, you joined Addgene's Advisory... 2013 Aug 11. pii: S0006-291X(13)01329-6. doi: 10.1016/j.bbrc.2013.08.013. ...

  17. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    Blog Post
    ...their MECP2-related plasmid tools with Addgene and share additional resources. Plasmid tools to facilitate...to partner with them to launch a Rett Syndrome Plasmid Collection and Resource Center for Rett Syndrome... Rett Syndrome in novel ways.  Addgene’s MECP2 Plasmid Collection and Resource Center will continue to... Disorders. Cell 152:984–996 . https://doi.org/10.1016/j.cell.2013.01.038 Guy J, Gan J, Selfridge J, Cobb...Editing. Cell Reports 32:107878 . https://doi.org/10.1016/j.celrep.2020.107878 Sinnett SE, Hector RD, Gadalla...Clinical Development 5:106–115 . https://doi.org/10.1016/j.omtm.2017.04.006 Tillotson R, Selfridge J, Koerner...Molecular Biology 432:1602–1623 . https://doi.org/10.1016/j.jmb.2019.10.004...

  18. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post
    ...Interested in using PTG plasmids in your research? Check out the PTG plasmids or plant expression systems...698-710. doi: 10.1016/j.molcel.2014.04.022. Epub 2014 May 15. PubMed.  Find the plasmids from this publication...common to prokaryotes and eukaryotes. Though the plasmids made by Xie et. al are specifically designed for....1420294112. Epub 2015 Mar 2. PubMed. Find the plasmids from this publication at Addgene Other Techniques...nbt.2908. Epub 2014 Apr 25. PubMed.. Find the plasmids from this publication at Addgene ...

  19. Lentivirus ddPCR Titration

    Type
    Protocol
    ...green) and negative (black) droplets Sample ID Plasmid ID Dilution Copies/20 µL RRE (FAM) Copies/20 µL...freezer blocks Vortex, VWR, 10153-688 Mini Centrifuge, Thermo Scientific, 10199-452 Reagents GeneJet Genomic...

  20. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...resource: Recombinant Antibodies! In addition to plasmids and viral vectors, you’ll now be able to order...et al., 2015). All of Addgene’s antibodies are plasmid-based and recombinant. Learn more about recombinant...other recombinant affinity reagents, share your plasmids via Addgene! Email [email protected] to learn...support, we have enabled scientists to easily share plasmids with each other. Together, we can transform the...Experimental Methods. Neuron 90(3):434-6. https://doi.org/10.1016/j.neuron.2016.04.030    Bradbury A, Plückthun A...
Showing: 321 - 340 of 416 results