We narrowed to 361 results for: reporter

1. Using Video to Share Your Science: We Share Science

   Type

   Blog Post

   ...total view count and videos published, YouTube reported a total of 865,995 views for the 926 video abstracts...

2. Changing Labor Laws Bring Increased Postdoc Wages

   Type

   Blog Post

   ...National Postdoctoral Association Institutional Policy Report 2014). However, ~30% set their minima lower. Furthermore...

3. Site Directed Mutagenesis by PCR

   Type

   Blog Post

   ...regulatory elements (to mutate promoters/enhancers in reporter constructs), in functional analysis of proteins...

4. Tips for CRISPR Gene Editing in Mice

   Type

   Blog Post

   ...expressing Cas9 and single guided RNA." Scientific reports 3 (2013). PubMed PMID: 24284873. PubMed Central...

5. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   ...single-use aliquots, and stored at -80°C. Some reports suggest rapidly freezing virus in a dry ice/ethanol...

6. Thoughts on the Future of Research 2015 Boston Symposium

   Type

   Blog Post

   ...(the current median postdoc starting salary as reported by Paula Stephan is $40,000) Increase Knowledge...

7. Important Considerations in Optogenetics Behavioral Experiments

   Type

   Blog Post

   ...differential participation in reward circuitry." Cell reports 8.6 (2014): 1857-1869. Pubmed PMID: 25242321. Pubmed...

8. Plan Your Experiment

   Type

   Guide

   ...promoter is typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive...vector or separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify...facilitates CRISPR/Cas9-based genome editing. Scientific Reports . 5 , 11315. PMID: 26066060 Zuris, J. A., Thompson...

9. Harnessing Bacterial Toxins for Allelic Exchange

   Type

   Blog Post

   ...genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason...

10. Five Popular Model Organisms

    Type

    Blog Post

    ...important gene co‑expression modules." Biomedical Reports 7.2 (2017): 153-158. PubMed PMID: 28804628. PubMed...

11. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    ...fluorescent proteins in budding yeast." Scientific reports 9.1 (2019): 2234. PubMed PMID: 30783202. PubMed...

12. March for Science

    Type

    Blog Post

    ...Immigrants National Science Foundation: Universities Report Fourth Straight Year of Declining Federal R&...

13. Evolution of Lab Techniques

    Type

    Blog Post

    ...already commercially available although they are reported to have high error rates (10). It is projected...

14. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    ...ten colors (see table to the right); in various reports, Sanes and Lichtman have observed from 90-160 distinct...

15. Finding Your Science Policy Path

    Type

    Blog Post

    ...science policy skills in graduate school via this report from the Scientific Citizenship Initiative.    ...

16. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    ...Large Gene Cassettes Using AAV Donor Vectors. Cell Reports 20:750–756 . https://doi.org/10.1016/j.celrep.2017.06.064...

17. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type

    Blog Post

    ...of reproducibility for preclinical studies, as reported by drug companies attempting to apply the findings...

18. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type

    Blog Post

    ..., et al. "One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated...

19. Hot Plasmids: Summer 2024

    Type

    Blog Post

    ...Integrase Gene Editing (PASSIGE), their previously-reported technique for precisely integrating large DNA ...

20. CRISPR Guide

    Type

    Guide

    ...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...mapping multiprotein complexes in human cells. Cell Reports . 13 (3), 621–633. PMID: 26456817 Doench, J. G....