Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 755 results for: rest

Showing: 321 - 340 of 755 results

  1. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...selection to isolate plants with the phenotype of interest. Genome engineering, on the other hand, allows...tumefaciens as a vehicle to deliver your gene of interest into a target cell line or organism (Presented...for Golden Gate mediated cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated...can be used as a vehicle to deliver your gene of interest (GOI) into plant cells. A) The system typically...

  2. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...far away brain regions expressing your gene of interest. This can cause issues in tracing studies, where...Promoters The promoter controls how the gene of interest will be expressed; it can be ubiquitous, cell-...particles per volume required to express the gene of interest, in the cells being studied, at an adequate level...Kügler, S. (2017). A MicroRNA124 Target Sequence Restores Astrocyte Specificity of gfaABC1D-Driven Transgene...

  3. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...screens to identify genes involved in a phenotype of interest. This blog post will discuss differences between...target sites occur ~1 in every 8 nucleotides, restricting gRNAs to 5 – 65% of the protein coding region... the lab. Importantly, for any modification of interest, it would be unwise to make conclusions on the...experimental strategy is clear: for any gene of interest, one should require that multiple gRNAs of different...

  4. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...confidently conclude the function of a gene of interest. Tools for RNAi Arguably the greatest advantage...required dsRNA complementary to your gene or genes of interest to trigger RNAi activation. A typical RNAi experiment...that contains sequence against your gene(s) of interest and the reporter. This approach greatly enhances...coupling silencing of APT and any gene target of interest, only organisms that are actively silencing to...

  5. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    ...comments. Additionally we’ve seen what topics are of interest to you, our readers. Below I’ve compiled a list... from the past year. A quick glance shows that interest in CRISPR continues to grow as the genome editing...community, so email us at [email protected] if you are interested. Addgene's Most Popular Posts of 2014 How...

  6. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...N-terminal tags, coding sequences of the gene of interest, C-terminal tags, plant terminators and resistance...component of Golden Gate and GreenGate are type IIS restriction endonucleases. They are like other endonucleases...their own lab: be very careful with the type IIS restriction endonuclease of choice, BsaI, which can be very...

  7. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...gene of interest in neurons. This ability to be paired with any Cre-controlled gene of interest makes pmSyn-BFP-Cre...may want to further control where your gene of interest is expressed.  A cell-type specific Cre can provide...

  8. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ...two phosphatases, PTPN1 and PTPN2, which are of interest as cancer immunotherapy targets but are embryonic...s laboratory at Harvard Medical School and is interested in improving CD8+ T cell responses to cancer....generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity. Nature Immunology 20, 1335–1347...

  9. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...expression. Cultures are then lysed and the protein of interest is purified using an affinity tag. Additional ...Determining the best way to express one’s protein of interest can save a lot of time later. Protein purification...C-terminal. Each set of plasmids uses the same restriction enzymes for cloning (see schematic below), enabling...

  10. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ... to on Twitter? Prior to writing this post, my interest in Twitter was fleeting. I’ve had an account for...Unfortunately, it never clicked for me. Recently my interest was renewed in part due to FOMO  but mostly because... scientist? If you don’t use Twitter, are you interested in getting started? Reply in the comments below...

  11. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type
    Blog Post
    ...With many interesting articles coming out in a myriad of journals every week, it can sometimes be difficult...plasmids and publications relevant to your research interests. We do this by promoting citation, supporting ...encourage you to fill out this form if you’re ever interested in writing your own blog post about a plasmid...

  12. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...to determine which job boards list positions interesting to you. Many job postings will result in hundreds...address how you are qualified and why you are interested specifically in the position. We frequently receive...cover letters. It is also important to express interest, even excitement, in a position. Everyone prefers...

  13. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ...somewhat unusual things. For example, I am not that interested in meeting movie stars, but when I met the astronaut...a scientist up there, but the panelists were interesting enough to make up for the somewhat naïve questions...their seminal, cooperative paper in Science.  Interestingly, this project started with their labs studying...

  14. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ... Short segment of DNA which contains several restriction sites allowing for the easy insertion of DNA....“vectors” or “constructs.” To insert a gene of interest into a vector, scientists may utilize one of a... a variety of cloning methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning...

  19. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...overcome. Conflict, and its inherent tension, is interesting. It’s what drives a story forward - and this ... the pieces that make you think, “Oh, that’s interesting! How did you solve that problem?” Those are the...your audience in with something that sparks their interest and makes them want to know more. This could be...

  20. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...collection, you'll likely find some proteins of interest for which you have several antibodies in stock...another, or if you’re looking for a protein of interest while it’s in a transition state or specific configuration... to validate that it recognizes the protein of interest in specific configurations or conditions. In those...
Showing: 321 - 340 of 755 results