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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 538 results for: sequence

Showing: 321 - 340 of 538 results

  1. Antibodies 101: Conjugation

    Type
    Blog Post
    ...approaches that involve modifying the antibody sequence or that rely on strong protein-protein interactions...Lysine residues are found throughout antibody sequences, up to 80 per antibody (Mueller, et al., 1988)...

  2. Neurodegeneration Research Collection

    Type
    Collection
    ...abnormally long polyglutamine sequences. These glutamine-rich sequences are prone to misfolding and aggregation...neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq platform. Mitchell et al. ...

  3. Deisseroth INTRSECT Collection

    Type
    Collection
    ...orthogonal recombinase (Cre and Flp) recognition sequences within synthetic introns. INTRSECT was first shown...: INTRSECT works by inserting short, intronic sequences into the open reading frame (ORF) of a molecular...molecular tool and adding recombinase recognition sequences (e.g. Lox sites, FRT sites) inside of the introns...The addition of these recombinase recognition sequences enables directional control of the portion of ...

  4. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...plasmid encoding the insert of interest as well as sequences necessary to encapsulate that insert into a viral...plasmid and the packaging plasmid caused the Psi sequence to be placed on the packaging plasmid forming ...composed of the wild-type 5’ and 3’ LTRs, the ψ sequence, a part of the env gene containing the rev response...

  5. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...domain can be engineered to target specific DNA sequences while FokI then induces a double strand break.... strategy of using a protein to recognize DNA sequences is not ideal. For the early generation gene editors...protein-based DNA-binding domain with a single-guide RNA sequence.  The Cas proteins, just like its predecessor ...

  7. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...fortunate to hand-deliver the model, the following sequence of events plays itself out: The researcher breaks...along the double-stranded DNA, recognize a PAM sequence, allow the complementary base pairing between ...

  9. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... pegRNA directs the Cas9 nickase to the target sequence where it nicks the non-target strand and generates..., if the edited 3’ flap is excised, the target sequence remains unchanged and available as a substrate...

  13. Lentiviral Prep Service

    Type
    Collection
    ...by an RNA guide (gRNA) that harbors targeting sequences. Browse our repository for a list of gRNA plasmids...plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used...

  14. AAV Packaged on Request

    Type
    Collection
    ...assessment, sterility testing, and viral genome sequencing....Purity assessment Sterility testing Viral genome sequencing (VGS) Find detailed pricing for your chosen plasmid...

  15. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...the PCR product should contain the edited BRAF sequence), and PCR-amplify the BRAF locus from control ... PCR product should contain the wild-type BRAF sequence). Then, mix the PCR products and use temperature... was challenging because you’re limited by the sequence of the human genome. I used the UCSC genome browser...

  16. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these sequences are not copied...

  17. Pooled Library Amplifications

    Type
    Blog Post
    ...ensuring the cells used are meant for repetitive sequences (such as STBL4 or Endura Duo), growing your bacteria... pCMV-Rep 78/68 SSM Library Amplification and Sequencing Protocol (DOCX, 32 KB) Screening Rinehart...

  18. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...itself and the degron off at the protease cleavage sequence. The tag is then shuttled to the proteasome due...the protein around, leaving a minimal additional sequence (7 kDa). This system also requires less genetic...

  19. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...more closely about the wealth of high-throughput sequencing (HTS) data we generate and maintain. It became...begin! Figure 1: Discussing next-generation sequencing at the Addgene bioinformatics journal club! ...
Showing: 321 - 340 of 538 results