We narrowed to 526 results for: sequence

... of DNA to create a synthetically designed DNA sequence. There are multiple methods of DNA assembly available...experimental and primer design based on fragment sequences and the polymerase used in the amplification. ...

... downstream methods, such as qPCR or next-gen sequencing. ChIP is an incredibly useful application for...suitable for isolated and purified DNA, such as sequencing or qPCR. Yay!  Where’s the challenge in that?...

...virus serotype 2-derived inverted terminal repeat sequences. Hum Gene Ther Methods. 2012 Feb;23(1):18-28. ...

...used by scientists to introduce different DNA sequences of interest into a bacterial cell or a host’s ...direct contact. The donor bacterium contains a DNA sequence called the Fertility factor (F-factor). The F-...

...scaffold and cloning sites for inserting the guide sequence (pSpCas9(BB)). The 2A-GFP allows for screening... plasmid for cloning and expressing new shRNA sequences. For more information, see the Addgene’s pLKO.1...

...are necessary for recognition and cleavage. This sequence flexibility indicates that gRNAs could be incorporated...tRNA-gRNA and tRNA-gRNA-tRNA, with short 5’ spacer sequences preceding gRNAs, to test for gRNA cleavage. gRNAs...

...heterogeneous when constrained by sequence identities in flanking sequence (“microhomologies”). NHEJ doesn...

...libraries will require access to next generation sequencing technology. These libraries are lentiviral ... including a tool for getting optimized guide sequences for activating any human gene, go to http://sam.genome-engineering.org...enabling systematic investigation of the cellular consequences of repressing or inducing individual transcripts...

...PEDAR strategy to delete and replace long genomic sequences. On the ninth day of CRISPR, Addgene gave to me...

...flanked by unique restriction sites so you can swap sequences effortlessly.  We built these plasmids for flexibility... flanking an RNA payload to insert the payload sequence into the genome. The team established that they...elements allowed for scarless insertion — no UTR sequence added to the genome (Figure 4). Figure...

... vector-free cells Genome integration Need to sequence cells to verify excision didn’t introduce mutations...integration Multiple transfections required Need to sequence to verify there’s no genomic integration Replicating...gene(s) of interest flanked by terminal repeat sequences, which the PB transposase recognizes. Following...can occur at the transposon insertion site, so sequence-verification is required. Non-integrating...

...editing for cleavage-free genomic knock-in of long sequences🎵On the 2nd day of CRISPR, Addgene gave to me:...

...approaches that involve modifying the antibody sequence or that rely on strong protein-protein interactions...Lysine residues are found throughout antibody sequences, up to 80 per antibody (Mueller, et al., 1988)...

...gene may have errors introduced to the nucleotide sequence during the DNA repair process. The addition of...recombinase catalyzes the recombination of DNA sequences located between two loxP sites. The loxP sites...

...signal sequence and to the mitochondria via citrate synthase’s mitochondrial targeting sequence. Under...

...diseases. Spontaneous mutations that change protein sequence affect arginine (Arg) amino acids more frequently...facilitated by CRISPR reagents and other specific gene sequences available from Addgene. Dr. McMurray hopes that...metabolic diseases with severe and life-threatening consequences. They result from defects in different steps...

...CRISPR and RNAi have helped researchers alter DNA sequence and RNA expression - but what if you just want...localization, binding to the appropriate promoter sequence and activation of gene expression. In addition...

...ability to optimize the antibody’s antigen binding sequence to improve binding and reproducibility. Some scientists...

...vector to use depends on the length of the spacer sequence between the half-sites (see Nuclease Expression...

...domain can be engineered to target specific DNA sequences while FokI then induces a double strand break.... strategy of using a protein to recognize DNA sequences is not ideal. For the early generation gene editors...protein-based DNA-binding domain with a single-guide RNA sequence.  The Cas proteins, just like its predecessor ...