We narrowed to 531 results for: sequence

...to target an ssRNA of interest, for example, a sequence specific to a virus or pathogen. Once Cas13 recognizes...recognizes and binds to the programmed sequence, it can promiscuously cleave surrounding ssRNA molecules...SHERLOCK. Cas12a is targeted to a specific DNA sequence, such as the Human papilloma virus (HPV) genome...

... of DNA to create a synthetically designed DNA sequence. There are multiple methods of DNA assembly available...experimental and primer design based on fragment sequences and the polymerase used in the amplification. ...

... downstream methods, such as qPCR or next-gen sequencing. ChIP is an incredibly useful application for...suitable for isolated and purified DNA, such as sequencing or qPCR. Yay!  Where’s the challenge in that?...

...eukaryote (S. cerevisiae) to have its genome fully sequenced There are several human disease models established...Genome manipulation K. phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for...

...behavior, many many images of brain regions, or sequences of microbial DNA they all seem to require some...biological tools. In addition to the researcher using sequencing technologies to study microbiome interactions...

...virus serotype 2-derived inverted terminal repeat sequences. Hum Gene Ther Methods. 2012 Feb;23(1):18-28. ...

...used by scientists to introduce different DNA sequences of interest into a bacterial cell or a host’s ...direct contact. The donor bacterium contains a DNA sequence called the Fertility factor (F-factor). The F-...

...scaffold and cloning sites for inserting the guide sequence (pSpCas9(BB)). The 2A-GFP allows for screening... plasmid for cloning and expressing new shRNA sequences. For more information, see the Addgene’s pLKO.1...

...are necessary for recognition and cleavage. This sequence flexibility indicates that gRNAs could be incorporated...tRNA-gRNA and tRNA-gRNA-tRNA, with short 5’ spacer sequences preceding gRNAs, to test for gRNA cleavage. gRNAs...

...heterogeneous when constrained by sequence identities in flanking sequence (“microhomologies”). NHEJ doesn...

...libraries will require access to next generation sequencing technology. These libraries are lentiviral ... including a tool for getting optimized guide sequences for activating any human gene, go to http://sam.genome-engineering.org...enabling systematic investigation of the cellular consequences of repressing or inducing individual transcripts...

...PEDAR strategy to delete and replace long genomic sequences. On the ninth day of CRISPR, Addgene gave to me...

...as cell health, DNA quality, transgene size and sequence, reagents, and other conditions. Less appreciated...account variations in the tracrRNA, the structural sequence of a gRNA. The Jacquere library was tested in ...Accounting for small variations in the tracrRNA sequence improves sgRNA activity predictions for CRISPR...

...flanked by unique restriction sites so you can swap sequences effortlessly.  We built these plasmids for flexibility... flanking an RNA payload to insert the payload sequence into the genome. The team established that they...elements allowed for scarless insertion — no UTR sequence added to the genome (Figure 4). Figure...

... vector-free cells Genome integration Need to sequence cells to verify excision didn’t introduce mutations...integration Multiple transfections required Need to sequence to verify there’s no genomic integration Replicating...gene(s) of interest flanked by terminal repeat sequences, which the PB transposase recognizes. Following...can occur at the transposon insertion site, so sequence-verification is required. Non-integrating...

...editor proteins across kinetics, editing windows, sequence preferences, and edit types. 3. From bench to ...-mutation editing strategies by enabling large sequence integration without triggering immunotoxicity....chromosomal structural variant analysis, targeted sequencing of computationally nominated sites, and validation...D., & Duffield, J. S. (2025). 3′-end ligation sequencing is a sensitive method to detect DNA nicks at ...

...editing for cleavage-free genomic knock-in of long sequences🎵On the 2nd day of CRISPR, Addgene gave to me:...

...ability to optimize the antibody’s antigen binding sequence to improve binding and reproducibility. Some scientists...

...vector to use depends on the length of the spacer sequence between the half-sites (see Nuclease Expression...

...signal sequence and to the mitochondria via citrate synthase’s mitochondrial targeting sequence. Under...