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We narrowed to 838 results for: tat

Showing: 321 - 340 of 838 results

  1. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ... that these mutations clustered in a particular region of the R-body. Further mutatgenesis of this region...understanding these complex processes can’t be overstated, we can also learn a lot from Nature’s simpler...dynamically rearranging into different polymerization states. Instead, R body monomers respond to pH changes...

  2. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...plates that don’t respond as expected, use of representative images, measuring the main outcomes in several...practices (QRPs) can quickly lead us to find statistically significant results that are not robust. Sampling...each hypothesis will be tested with a specific statistical test.  Preregistration makes clear the distinction...to present that work using hypothesis testing statistical tools that generate a p-value.  As an example... of Life Sciences at Rutgers University. His dissertation and research background is in citizen science...

  3. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...vivo and in vitro, as well as to insert targeted mutations through homologous recombination. To further increase...protoplasts, Xie et. al observed slightly higher mutation rates than they’d previously reported in protoplasts..., they found that 50% of plants had biallelic mutations at all five loci. This highly efficient multiplexing... space of a standard pol III-gRNA, which will facilitate CRISPR/Cas9 multiplexing in systems with limited...overlapping activity. Multiple gRNAs can also facilitate deletions of other regions, such as noncoding...

  4. RNA Extraction Without A Kit

    Type
    Blog Post
    ...trick is to precipitate the RNA using lithium chloride. LiCl solutions selectively precipitate RNA, but ...cesium-chloride gradients, water-saturated phenol, sodium acetate, and chloroform are added to the homogenate and...is extracted and RNA can then be isopropanol precipitated. This method reduced the time it took to isolate... results of a downstream application such as quantitative PCR to assess gene expression. There are several...

  5. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...DNA-protein interactions or to analyze chromatin states (CHIP-seq, FAIRE-seq, Cut & Run) and to detect ...interactions has lagged behind, likely due to limitations of current means to detect RNA-protein interactions...interacts with RNA. Methods like cross-linking immunoprecipitation (CLIP) identify RNA-protein interactions ...such as during drug action or cell signaling. Limitations of RaPID Like other BirA*-based proximity labeling...early-onset cataracts, is characterized by point mutations in the Iron Response Element (IRE) RNA motif of...

  6. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...load The Wikipedia definition of cognitive load states: “cognitive load refers to the used amount of working...re unaware that we are leaning on an unspoken expectation of our audience knowing foundational concepts...feedback in a variety of ways, like having them restate or summarize the concepts you’re covering. Don'...just really tired and needed a slower, simpler presentation than I had planned; other times students were...fired up and enthusiastic and needed a faster presentation with more information than normal. I had to ...

  7. Working with Nuclear Receptors

    Type
    Blog Post
    ...and disease states, their circadian regulation, as well as in important physiological states such as adipogenesis...which then return information such as nutritional status. Within the animal kingdom, this information is...that interact directly with NRs) exist in steady-state complexes with secondary coregulator (or co-coregulator...whole complex.  For example, the phosphorylation status of SRC-3 defines its association with other members...

  8. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ... PE6e-g editors with mutations to the Cas9 domain but found that these mutations only improved efficiency...created and evaluated 19 PE1 variants with RT mutations known to increase activity, enhance binding between...additional nuclear localization sequences; and two mutations in Cas9 previously shown to improve nuclease activity...effective prime editor proteins, but found that the mutations that emerged depended strongly on the target edit... ~ ~ ~ ✓ ✓ ✓ R221K N394K Cas9 mutations Improve Cas9 nuclease activity ~ ~ ~ ...

  9. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...antigens not present on the cell surface. The presentation of antigens can also be affected by cell fixation...quantified using the Attune NxT Flow Cytometer with representative images showing the staining intensity in the... Correctly interpreting your data therefore necessitates careful design of RNAi sequences, confirmation...validation is critical to ensuring accurate interpretation of your results. Therefore, taking advantage...Uniprot ID P21579) for use in western blot, immunoprecipitation, immunofluorescence and flow cytometry. F1000Research..., Robertson, J., & McPherson, P. S. (2019). Implementation of an antibody characterization procedure and...

  10. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...poster, you shouldn’t hesitate to be creative. After all, your poster is a representation of your work and ...most prominently, making it the star of your presentation. Portrait. This style displays a poster vertically...Morrison, a psychology doctoral student at Michigan State University. The idea is that the main research finding... center of the poster in a large font. Hence, spectators can read the text from many feet away and immediately...

  11. A Look at Addgene's QC Process

    Type
    Blog Post
    ...components of the plasmids to partial, full, or annotated plasmid sequences. These sequences can also be...the deposited sequence(s) in SnapGene. SnapGene annotates the plasmid maps based on SnapGene’s “Common Feature... function of the plasmid. This includes point mutations, frame shifts, missing elements, and nucleotide...plasmid that are less likely to affect function when mutated; elements that are difficult to sequence; approaches...Sequence discrepancies are not always a result of mutations in the plasmid, but can arise due to errors in...

  12. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...range of motion, a commutator allows the patch cable to connect to the laser and rotate 360° without a loss...wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources discussed in post...variant you wish to use to determine the ideal excitation). Lasers can be purchased through Laserglow, ...channel, Channelrhodopsin 2 (ChR2), exists in several states: closed/light receptive, open, closed/light insensitive...

  13. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...effects this mutation has on locomotion in our computer model to the effects of this mutation in the real...knowledge sharing, in the form of workshops, presentations, and lectures. There are currently around 1,...to modify the worm genome and make additional mutations in our worms.  We are on the lookout for genome...contributes each month - with only occasional supplementation with grant funds, money is always tight. Of...

  14. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...from New England Biolabs. Golden gate assembly limitations Embraced by the synthetic biology community, ...or two-step assemblies. We have reduced these limitations through the use of engineered Type IIS restriction...shown above) are flanked by BsaI sites in an orientation that results in 5’ four base overhangs after ...transformants onto the selection plate. Figure 2 shows representative transformation plates obtained from 1-, 12-...standard reaction conditions. This data set allows quantitation of sequence-dependent ligation efficiency and...

  15. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...Addgene makes this easy by providing a template citation for you to use in your Materials and Methods and...Addgene catalog number to easily find template citations for Addgene plasmids you have used in your research...depositor has indicated that there is a particular mutation found within the gene/insert, this information...questions about our plasmid pages, please don't hesitate to contact us at [email protected] Resources on... on the Addgene Blog Read about Our New Citations Feature Check out Our Walkthrough of the Deposit Process...

  16. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...expression? Are you trying to create a single point mutation? Do you want to create a fusion with a reporter...generating knockouts or knock-ins, or introducing mutations and tags, while a catalytically dead dCas9 can... Base editors can help you make precise point mutations near the gRNA target site. Choose an appropriate...repair (HDR) template to create knock-ins, point mutations, or to add a tag, you will also need to deliver... the expression system you choose in many ways dictates the best method for introducing those CRISPR components...

  17. Plasmids 101: Protein tags

    Type
    Blog Post
    ...chemical and peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant...recombinant proteins for one-step purification with glutathione, can also protect against proteolysis. That’s... then your first step towards detecting, immunoprecipitating, or purifying that protein may be to fuse...Purification and Stability Good for purification with glutathione; protects against proteolysis, but may reduce...column runs. The TEV protease itself, with various mutations used to increase its stability activity, can be...

  18. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...diversity in fluorescence brightness, intensity, excitation and emission spectra. The lab of Dimitri Deheyn...group to perform independent two-color optical excitation of neurons. The paper describes five previously...variety of contexts. In order to overcome these limitations, they evolved and characterized an improved peroxidase...Clonetegration could become a “valuable technique facilitating genetic engineering with difficult-to-clone ... sites that flank a DNA module in an inverse orientation, so that multiple DNA components can directionally...synaptic partners) technique based on functional complementation between two non-fluorescent split GFP fragments...combinations (depending on the number of copies). This facilitates the distinction of neighboring cells in imaging...

  19. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...devised means of co-opting phage genetic systems to facilitate homologous recombination in bacteria. These quirks...non-edited cells to survive have inactivating mutations in the Cas9 or spacer sequence, and these rare...pneumoniae and can be used to generate multiple mutations simultaneously (Jiang et al. 2013). Genome editing...recombineering, with 96-99% efficiency for point mutations and 66% efficiency for small insertions. Multiplexing... E. coli, the system was used successfully in Tatumella citrea, another species of Enterobacteriaceae,...they were effectively able to introduce point mutations in P. aeruginosa, Pseudomonas putida, Pseudomonas...

  20. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...population and track connections to that population. Limitations of common genetically encoded neuronal tracers...attached to a protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific... A new and improved neuronal tracer With the limitations of epitope tags and fluorescent proteins, it ...track with these classic probes. To overcome the limitations of existing FP and epitope tags, researchers ...Bobby Henderson, a physics graduate from Oregon State University. Find the Spaghetti Monster probes at...
Showing: 321 - 340 of 838 results