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We narrowed to 800 results for: tec

Showing: 321 - 340 of 800 results

  1. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...analysis blog post. Colony PCR Colony PCR can detect the presence or absence of DNA using lysed bacterial...plasmid. In this case, you’d be using colony PCR to detect your insert, either by using primers that are specific...time-intensive than Sanger sequencing or the other techniques mentioned above. In molecular biology, cloning...By using a variety of screening and selection techniques, you can increase the chances of finding the ...

  2. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...revolutionary physical approaches. However, with each new technical achievement, the complexity of the device increases...PhD, I have been convinced that most microscopy techniques can be replicated with commercially available...available tools and components. Rapid prototyping techniques such as 3D printing, on-demand CNC milling, and... and PCB manufacturing. These newly developed techniques are the key enablers for making assemblies open-source...

  3. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...at each locus, as well as large deletions. They detected fragment deletion with 4-20% frequency at the ...you have any questions or thoughts about this technology, leave them in the comments below.  References...plasmids from this publication at Addgene Other Techniques for gRNA Multiplexing Multiplexed and programmable... Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Jun;32(6):569-76. doi: 10.1038/nbt.2908...

  4. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ... but this skill is not just about reacting to technical issues. This skill also comes into play while ...encounter. As you spend time in a lab learning techniques and taking on independent projects, you will ...solutions. Ultimately, problem solving is less about technical know-how and more about how you approach new challenges...important. Paired with your other skills, both technical and transferable (i.e. teamwork and time management...

  5. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    .... We also opened up applications to research technicians and undergraduate researchers, in addition to...Abhi Aggarwal is a postbaccalaureate research technician in Kaspar Podgorski’s lab at Janelia Research...variants of iGluSnFR3 in vivo and have used it to detect glutamate release from hundreds of synapses at ...believe that improved biosensors or biosensors that detect new redox metabolites are vital for researchers...

  6. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...innovations” stimulates excellence in science and technology by integrating sex and gender analysis into research...anything unique to male stem cells. Nor did they detect important differences in function between male ...international expert on gender in science and technology and currently directs the EU/US Gendered Innovations...Nations on the topic of “Gender, Science, and Technology” in 2011 and again in 2014. Resources on the ...

  7. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...to your cloning efforts. Golden Gate cloning technology relies on Type IIS restriction enzymes, first...Voytas groups described a new Golden Gate-based technology for genome editing which allowed for the ordered...efficiently in just a few steps. More recently, CRISPR technology has adapted Golden Gate cloning for inserting...Expression of gRNAs Choosing a Molecular Cloning Technique  ...

  8. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...actively pursue. Proposal writing: Improves technical writing and communication skills, Elucidates... the limitations of current state of the art technologies. Therefore, take time to review the literature...insight into what the desirable features of future technologies should offer in order to build upon and address...proposal to test hypotheses and develop novel technologies! If data from a publication or preliminary data...

  9. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...at the Korea Advanced Institute of Science and Technology (KAIST) and Institute for Basic Science (IBS)...primarily to the cell body with very little signal detected along the neurite (Figure 1.) Moreover, the group...Zhang’s lab is developing new super-resolution techniques for live-cell imaging of the dynamic changes ...spatial and temporal organization. The design use to detect PKA activity can be generalized to measure phosphatase...

  10. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...presentation as an opportunity to demonstrate technical and transferable skills that make you qualified...perfect science talk Be prepared for potential technical difficulties. Have your presentation on a flash... quiet place to take your video call.  Expect technical difficulties. To try and circumvent this, test...from HR carefully. And even if you don’t have technical challenges, your interviewers might.  You’ll probably...

  11. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...Brain Research Through Advancing Innovative Neurotechnologies (BRAIN) Initiative Cell Census Network, the...purposes. The Wu lab mostly uses whole-mount IHC techniques on rodent brains and is expanding to more complex...antibodies (See Fig 1.). rAbs are a relatively new technology that takes advantage of DNA sequencing to turn...antibodies. Since recombinant antibodies are a newer technology, it is important to show through qualitative ...

  12. Hot Plasmids February 2024

    Type
    Blog Post
    ...Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells remain difficult...using peptide-assisted genome editing. Nature Biotechnology, 10.1038/s41587-023-01756-1. https://doi.org...monomeric StayGold fluorescent protein. Nature Biotechnology, 1–4. https://doi.org/10.1038/s41587-023-02018...throughout the non-human primate brain. Nature Nanotechnology, 18, 1241–1251 (2023). https://doi.org/10.1038...

  13. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...that can be tweaked to target ncRNA. CRISPR/Cas9 techniques and targeting methods Knock-out: Targeting short...targeting of long non-coding RNA genes. Nature biotechnology. 2020;38(5):573. doi:10.1038/s41587-020-0428... cells by Cas9 targeting of splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038...Non-Coding RNAs in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting. Front Plant...

  14. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...molecular biologic techniques to further the understanding of epigenetics; techniques include chromatin...and bisulfite sequencing. However none of these techniques can be used for in-situ research of dynamic modifications...reporting methylation status of these sites. This new technology opens up a new way to visualize changes in gene...

  15. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...variety of research sectors to provide open source technologies and spaces where interested parties can do research...fantastic optics and fluidics platform it contains. Biotech equipment becomes obsolete amazingly fast, but ...is a passion for learning and having fun with technology, so feel free to contact your local space and...Biocurious.org, Derek is bringing cutting edge technologies to Victoria, BC. Follow Derek on Twitter @derekja...

  16. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ... – so much so that there was little range for detectable improvements in the methodology. To further push... While T4 DNA Ligase, the mainstay of most biotechnological cloning efforts for over 50 years, prefers...work, will fuel additional efforts to push the technique forward. Many thanks to our guest bloggers Becky... efficiency Learn about other plasmid cloning techniques Resources on Addgene.org Find basic molecular...

  17. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...many labs still love using them! With so many technologies currently available, you now face the challenge...Alternatively, you might consider Gibson cloning. In this technique, you first perform a round of PCR to generate ... there are never any guarantees, some cloning techniques do tip the scales in your favor. For starters...ligation-independent PCR cloning method." BMC biotechnology 11.1 (2011): 92. PubMed PMID: 21992524. PubMed...

  18. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...sequencing capabilities and Seqwell’s plexWell technology, we can now perform NGS at Addgene to sequence... we mixed into the original AAV sample at 5%. Detecting 5% contamination is a good start, but to determine..., with a high enough sequencing depth, we could detect contamination as low as 0.1% (Guerin et al., 2020...perform a deeper analysis of the data. Our serotype detection software confirms the serotype of the prep by ...

  19. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...   Novel protein cage provides simple enzyme protection in yeast bioproduction pathways By Gabrielle ...(MPyV VLPs ). This artificial nanoparticle can protect proteins from unfavorable interactions in the cell...pathway (myo-inositol oxygenase), providing enzyme protection while still allowing substrates and products ...system provides a simple, efficient method for protecting a single enzyme that drives biosynthetic pathways...

  20. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ... low, ranging from undetectable to 0.1%. In contrast, new genome editing techniques such as CRISPR are...the endogenous gene. Here, we'll describe the techniques and the plasmids used to inactivate specific ...specifically with a gRNA). Be sure to keep these techniques in mind when choosing a knockout strategy! Knockout...
Showing: 321 - 340 of 800 results