...cars become more technologically advanced leaving earlier versions obsolete, the techniques we use in lab...brainstorming new technological innovations. Let’s take a look at three historical techniques: southern blotting...classic techniques that allowed for many seminal scientific discoveries (5, 8). While newer techniques have...these, these techniques still have useful applications and have informed many current techniques. For example...recombinant DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly progress... time and money. Yet, knowledge of historical techniques comes in handy whether you are perusing classic...inventor Edward M. Southern (1), can be used to detect DNA identity, size, and abundance. If a scientist...

...dramatic spread of the Cas9 technology: "The rapid adoption of the Cas9 technology was also greatly accelerated...you think these technologies are going? What is the next hot molecular biology technology? Have you deposited...unique position to observe and quantify how new technologies are being disseminated through the scientific... plasmid sharing In a recent review on CRISPR technology, for example, the authors Patrick Hsu, Eric Lander...

...companies have real scientists answering their technical support questions (besides Addgene, I know both...emails and phones). We can’t be experts on every technique or experiment, but a picture of a gel or a sequencing... is answerable, particularly when it comes to technical questions. BUT, I do believe that scientists who... technical support...

...research materials and information. Many of our technology platforms like HaloTag® Fusion Protein, codon-optimized...Luciferase-based tools are fantastic examples of technologies that can accelerate your research. The creation... Oplophorus luciferase by Promega’s Advanced Technologies group as well as evolution of the coelenterzine...RLuc reaction. In fact, NLuc is bright enough to detect endogenous tagged genes generated through CRISPR...I’ll cover two great applications of the NLuc technology. The first couples NLuc to fluorescent proteins...require external light sources. Unfortunately, detecting traditional luciferases’ dim light emissions requires...autofluorescence, and perturb biological phenomena. The technique of coupling FPs with Luciferases can overcome ...

...toolkit based on the Multisite Gateway® technology from Life Technologies. The MultiSite Gateway Kit is a...continually impressed with the amazing plasmid technologies developed by our community of depositors. With...domain. Feng Zhang's group utilized the bi-lobed architecture of Cas9 to engineer a series of "split" cas9...plasmids 62887 & 62888) Zetsche et al., Nat Biotechnol. 2015 Feb 2;33(2):139-42. doi: 10.1038/nbt.3149... system. The ease and flexibility of the LACE technology makes this system widely accessible for many ...imaging and inducible gene expression Brilliant technologies adopt basic biological concepts and employ them... the Weissman lab have teamed up and advanced technology in the fields of single molecule imaging and ...

...Addgene which allow scientists to use CRISPR-Cas technology to perform genome-wide knockout screens. These... Looking for background information on CRISPR technology? See our improved CRISPR-Cas Guide. Wang et...Jan 3;343(6166):84-7. Koike-Yusa et al., Nat Biotech. 2013. Dec 23.   Highly bright and extremely...new tool for peptide-peptide locking. The new technology is known as SpyLigase and is a protein domain...E690-7. For further reading about SpyLigase technology, read the Addgene interview with Mark Howarth...events occur in a physiological setting. Genetic techniques typically rely on overexpression or knock-down... and his team at the Institute of Science and Technology Austria, decided to take a novel approach to ...

...alteration. Molecular cloning techniques are some of the most widely used techniques in the biological sciences...restriction cloning is one of the oldest of these techniques and is a multi-step process consisting of digestion...performed by many labs, a variety of other cloning techniques with higher efficiency and simpler procedures... still encounter several struggles with these techniques: unwanted mutations due to excessive PCR cycles...FastCloning is a simpler yet reliable cloning technique that was developed by Li, et al., in 2011. This... inserts. However, with any PCR-based cloning technique there is a chance of introducing an unwanted mutation...some tips for high efficiency cloning with this technique: Amplification efficiency is highest when using...

...get all the attention.There are numerous ways to detect DNA-protein interactions or to analyze chromatin... states (CHIP-seq, FAIRE-seq, Cut & Run) and to detect protein-protein interactions (yeast-two hybrid,..., likely due to limitations of current means to detect RNA-protein interactions. To address this need,... Stanford created the RNA-protein interaction detection (RaPID) method. RaPID borrows the E. coli biotin...but it’s toxic to tissues and model organisms. Detecting RNA-protein interactions with RaPID RaPID is similar...*s in the context of RaPID for their ability to detect a known RNA-protein interaction.  In as little ...a scrambled RNA motif control). BioID2 had no detectable signal at the same time point. E. coli’s BirA...

...names for technical support roles. Examples are Technical Service Representative, Technical Support Specialist...these departments from technical support. Technical support careers in brief Technical support careers are...worked as a technical support specialist for 4 years. Read on for insights from my time in technical support...products successfully. In technical support you may have to answer technical enquiries such as: “Can I... areas of expertise in technical support positions. When applying for technical support positions, you...familiarize yourself with technical support. Even having personal experience with technical support through the... do well in a technical support role. What general characteristics do jobs in technical support have? ...

... sectioning techniques due to a higher photon flux via the more efficient camera detector and lack of ...been a very slow imaging technique but recent advances in scanner technology and parallelization (7) have...sports photographer at the Super Bowl, a medical technologist taking an x-ray, or a biologist imaging the ...emerged as the dominant light microscopy contrast technique in modern biology (1). When performed correctly...block out of focus fluorescence from reaching a detector or restricting fluorescence to specific excitation...super-resolution (3). Fluorescence microscopy techniques   Fluorescent Widefield Microscopy Fluorescent...Microscopy was the first fluorescence microscopy technique to incorporate optical sectioning. Optical sectioning...

...ability to create a DNA detection platform called DETECTR. How does DETECTR work? DETECTR works similarly to...samples. Find the DETECTR plasmids here! Applications of DETECTR in diagnostics DETECTR allows for simple...published this in Nature Biotechnology. Like the SHERLOCK detection system, DETECTR has not been approved...nucleic acid detection is crucial for clinical diagnostics, genotyping, and biotechnological advancements...nucleic acid detection however, either lack the sensitivity or the specificity to detect nucleic acids...CRISPR Trans Reporter (DETECTR) (Chen et al., 2018). Both SHERLOCK and DETECTR harness the promiscuous...atomolar levels, within one hour. DETECTR thus has the ability to rapidly detect nucleic acids with high selectivity...

...place to provide community protection. Toehold switches for pathogen detection In 2014, as postdoctoral...these combined technologies, we built paper-based synthetic gene networks for the detection of antibiotic...also demonstrated our technology on active virus, as well as shown that we can detect the virus in a clinically...Harvard University), we developed two new enabling technologies for the field of synthetic biology. The first...for the rational design of RNA sensors that can detect virtually any RNA sequence (Green et al, 2014)....sensors to be used outside of the laboratory. The detection threshold for these RNA sequences (3 nM), however...blood, urine). Adapting toehold switches for Zika detection We and other members of the lab had been working...

...engineering through biotech. After graduating with a masters of science in Horticulutre Biotechnology, he did his...his PhD in Plant Breeding, Genetics, and Biotechnology at Michigan State University with the backing of...works as a lecturer in molecular genetics and biotechnology for Horticulture and Forest Crops at the Lilongwe...sciences and agriculture 5:47-9:35 : Kingdom's biotechnology research at Michigan State University 9:36-10...Kingdom on the difficulties surrouding and need for biotech research in Malawi 11:41-14:53 : Kingdom's current...

...network with other technicians/company representatives. I got to talk with a technician from Neon Therapeutics...looking to try out a new technique or reagent! Racheal Komuhendo is a Lab Technician at Addgene who is interested...As a tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, ... we get many opportunities to learn from other biotech companies at workshops and can use what we learn...lab and office space shared by numerous emerging biotech companies. The space was modern with clean bold...heard about how LabCentral aims to enable emerging biotech companies, I was excited to go there because both... NEB gave a brief presentation describing the technology. Then we split into pairs to actually try out...

...proteins to study. These are highly useful for the biotech industry, which is in the process of capitalizing...other modifications to their bases in order to protect their DNA from host cell nucleases (4). To further...event that genome sequencing can be done, this technique can also help pinpoint the genes within a sequenced...live cells The following is a description of a technique published by Simpson, Sacher, and Szymanski in...membranes well with PBST to remove unbound cells. To detect bound cells, simply lay each membrane on an agar...

... In Situ Hybridization (WISH) WISH is a technique that detects the temporal and spatial localization of...Scientists routinely use techniques to alter gene expression or to label specific cells, but there are...experience is focused on classical embryology techniques such as basic observation and dissections. Students...Students don’t usually perform more modern techniques used in genetics or molecular biology because the...through time or after genetic manipulation. The technique involves using synthetically made RNA (riboprobes...binding (via hybridization) specific antibodies that detect the mRNA-riboprobe complex are added to the tissue...labs to bypass these steps and focus on the WISH technique. The expression patterns of 11 different planarian...

... distributed plasmid technologies pSV-PGC1 - Ok, it's not actually a technology, but pSV-PGC1 was Addgene's...institutions. This has given us a unique window into technology trends in the life sciences. In this post, we'll...give you an inside look at the Top 10 plasmid technologies distributed through Addgene over our first 10...genetically encoded calcium indicators were the hot technology of the year in our collection. CRISPR/Cas9 - ...Year of the CRISPR." The newest genome editing technology has been used and modified by so many labs and...

... complementary strand was degraded. Beta: Beta protects the ssDNA created by Exo and promotes its annealing...bacteriophage, which is not a common molecular biology technique. However, an advantage of this method is that ...

....   Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR assay Sensitivity...R​eporter un​LOCK​ing) technique Sensitivity: Single molecule per milliliter can be detected – in the zeptomolar...-1 and SARS-CoV-2 detection, AIOD-CRISPR without preamplification was able to detect as low as 1.2 copies...visualization. In this technique, however, instead of using lateral flow strips to detect the cleavage of fluorescently...: FnCas9 Editor Linked Uniform Detection Assay (FELUDA) is a technique that uses FnCas9, a protein that...CY (2020) CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587...times, have led researchers to turn to other technologies like CRISPR. Figure 1: An overview of...

...approach you choose, it’s essential to consider the technical limitations of each approach and to follow best...