... entered Feng Zhang’s lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like...modular optogenetics tool The researchers also showed just how flexible their LITE system could be by applying...and epigenetic states." Nature. 500, 472–476 (22 August 2013).   , with the addition of histone modifiers...

... produce monoallelic deletion clones. Cells with just a single intact copy at a given locus may be useful...in unprecedented fashion. Targeted deletions are just one of many available strategies by which biologists...Characterization of genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR...

...2020, Addgene's Europe office no longer handles customer support inquiries. The office is now focused on...discoveries. At your service: Addgene’s London office Just a few weeks ago I became the newest Addgene Outreach...worked in the the UK, California, Africa, and Australia, viewing science as a global collaboration. This...

... etc.). To individually isolate a protein, there must be a special feature about that protein that no ...function for downstream applications. Tag cleavage must be performed after primary purification and requires... tags can also be used for protein purification, just as affinity tags can sometimes be repurposed for...

...voltage indicator (GEVI) called JEDI-2P. Using a custom, high-throughput screening platform to apply saturating...Find JEDI-2p plasmids here!  Liu, Zhuohe et al. Sustained deep-tissue voltage recording using a fast indicator...to guess that the Myc-tag is one of them (and not just because of the title!) The Myc-tag is a string of...

...Department at Iowa, but kept up a freelance writing hustle on the side. “I actually stumbled upon, about 6...’s persistence paid off: “I spent about 6 months just editing and searching for a job and I stumbled upon...experiment with new careers while in grad school And just for good measure, here’s a few other posts about...

... SB transposase system - one set of vectors for robust constitutive expression (pSBbi derivatives) and...generation of stable transgenic cell lines which can robustly express your gene-of-interest, either constitutively...hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates. Nat Genet....

...how much has been done with CRISPR technology in just 2 years and excitement about how much more there...synthetic biology. I've been answering biology customer service questions for nearly 7 years, and my colleagues...represented engineering first and foremost, from the custom software used to design complex genetic circuitry...

...retroviral backbone. This is considered to be a more robust expression system and has its advantages; however...cassette and visualization vehicle to conduct your custom experiment. Fly vectors The vast majority of fly...guidance for choosing your perfect plasmid backbone. Or just ask –  leave a message in the comments below and...

... the technological biological blockbuster known as the CRISPR (clustered regularly interspaced palindromic...in E. coli in 1987 (1), the concept that these clustered repeat sequences operated as a safeguard against...

...creativity, changed the outcome of the project, or illustrates the value of the work and then build a story ...   Fig. 2: The structure of a story - not just for fiction! Image credit: Kenneth Rowe, under the...relate to. It doesn’t need to be anything fancy; it just needs to capture your audience’s attention enough...

...begins, enabling a plasmid to reproduce itself as it must to survive within cells. The replicons of plasmids...will produce many plasmid copies and others produce just a few copies depending on how they are regulated...give higher copy numbers since the bacteria have just reached stationary phase, but the cells have not...

...experiments, making AAV starts with some plasmids. You just need three plasmids to start making AAV:  the packaging...The WPRE is located at the end of the AAV genome, just upstream of the final ITR.  polyA  The polyadenylation...The polyA is located at the end of the AAV genome, just upstream of the final ITR and downstream of the ...

...sequences from a shared database of custom common features. Second, customers have asked for a way to annotate... involves iterative refinements in response to customer feedback. An example of this approach is SnapGene...

...the first to perform neutralization studies after just the first vaccination and after both vaccinations...importance of the second vaccine dose in mounting a robust immune response. The Balazs lab was quick to deposit... Cele S, Gazy I, Jackson L, Hwa S-H, Tegally H, Lustig G, Giandhari J, Pillay S, Wilkinson E, Naidoo Y...

...can predict whether a gene is natural or synthetic just by looking at its sequence. MIT collaboration for...codons also are not good predictors because they are just that: rare. “Nature has many genes where there are...engineered DNA. Both of these groups have generated robust tools that have practical applications in monitoring...

... or not your idea is viable.  Go to experts you trust and pitch what you hope to build.  Then ask for ... pitch we made, we considered the feedback and adjusted our business model accordingly until we had a ...You need a dedicated team that you know well and trust.  This will allow you to discuss each project and...

...blots and cloning, I decided to pack it in. I had just started month 8 of my 3 year Post-Doctoral contract...reading for my current sales position interview was just as important. The problem was I knew I had a knowledge... and allows you to work with and alongside the customer. From there a move with either promotions or further...

... quick zap of the proper color light, you can do just that.  Photosensitizing dyes and proteins have been...injecting adult fish with the dye. Embryos exposed to just 12 minutes of illumination had fewer mCer3-labeled...KillerRed reaching greater than 75% photobleaching after just 1 minute of exposure. MG-2I-dL5** experienced minor...

...in-depth research is applicable to a wide range of industries and is a highly desirable quality sought after...skills include: Science Reporter Scientific Illustrator Research and Development Scientific Advisor..., including: Livestock Breeder Scientific Illustrator Plant Breeder Zoological Worker Conservationist...