We narrowed to 655 results for: EPO

... extract as much information as possible from depositing laboratories and associated publications, but...would ‘spot-check’ critical regions of plasmids deposited with us via Sanger sequencing, while more recently...Generation Sequencing (NGS) technology. As our repository continues to expand, we realized the need to ...scale our lab work as we grew. As the number of deposited plasmids increased, the accompanying sequencing...

...content_id":null,"href":"https://www.addgene.org/depositor-collections/allen-institute-aav-enhancers/","href_with_scheme..."href_with_scheme":"https://www.addgene.org/depositor-collections/allen-institute-aav-enhancers/","type...evaluate the resulting expression of the fluorescent reporter, the brain tissue was extracted, sectioned, and...expression from mouse to primate neocortex. Cell Reports, 34(13), 108754. https://doi.org/10.1016/j.celrep...

...of interest and co-expressing a cytosolic ZtSH2 reporter, they observe fluorescence localizes to the plasma...And orthogonal sensors with different ITAM/SH2 reporter pairs allow multiplexed measurement of different...10.1101/2022.08.13.503850.  iGECInano NIR calcium reporter Near-infrared (NIR) fluorescent proteins promise...photochemical characteristics than previous NIR calcium reporters iGECI and NIR-GECO2G, and its small size (~1500...

... (Specific ​H​igh ​s​ensitivity ​E​nzymatic ​R​eporter un​LOCK​ing) technique Sensitivity: Single molecule...DNA polymerase, recombinase, and the ssDNA-FQ reporters that exhibit fluorescence upon cleavage. Upon ... results in the trans cleavage of the ssDNA-FQ reporter resulting in fluorescence. Cas12a binds all the...results in the continuous cleavage of the ssDNA-FQ reporter. The resulting fluorescence can be observed under...

...in the same lane, and the resulting fraction is reported. Figure 2: Normalization with loading controls...equally strong, so the normalized value simply reports which POI band is stronger. The right gel has exactly...abundance on your membrane, which would be directly reported by band intensity. Researchers use later steps...perturbed by the experiment Unfortunately, multiple reports suggest that common housekeeping proteins are influenced...

...integrin antibodies available through Addgene’s repository. The collection includes 26 unique antibodies...in competition with other similar integrins, as reported by IPI and the Springer lab (Hao et al., 2024)...In this study, we, along with the Springer lab, reported the binding specificity, kinetics, and affinity... by IPI. Our integrin antibodies are the first deposit from an expanding collection of readily accessible...

...stage-specific promoter and design a UAS reporter construct, the reporter will only be expressed where and/or...UAS to examine the expression of reporter genes, or to combine reporter expression from one promoter with...

...that uniquely express selectable and/or sortable reporters in certain states of interest. For example, in...above (Sanger sequencing, qPCR, and fluorescent reporter-based) each come with their advantages and disadvantages...the most convenient strategy is the fluorescent reporter based method since it lends itself to cheap, quick...detection with flow cytometry. However, expressing reporters will consume cellular resources and therefore ...

...approaches may become even more powerful following a report that one can use linear repair templates (ie. PCR-derived... 2014). A further development of this approach reported in 2017 uses the direct injection of a CRISPR/...templates (Farboud et al., 2019).   The Mello lab also reported that modifying the 5’ modification of oligos used...mixtures (Doksin et al., 2018). The Mello lab also reported efficient editing using a co-injection marker ...

...protein analysis products. You can find plasmids deposited by Promega here.    References and resources ...ligands for dopamine D2/D3 receptors. Scientific Reports, 10(1), 21842. https://doi.org/10.1038/s41598-020...fluorescence-based ligand-binding assay. Scientific Reports, 6, 25769. https://doi.org/10.1038/srep25769  Grimm...mitochondrial dynamics in mammalian cells. Biophysics Reports, 3(4–6), 64–72. https://doi.org/10.1007/s41048-...