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We narrowed to 653 results for: EPO

Showing: 171 - 180 of 653 results

  1. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...of interest and co-expressing a cytosolic ZtSH2 reporter, they observe fluorescence localizes to the plasma...And orthogonal sensors with different ITAM/SH2 reporter pairs allow multiplexed measurement of different...10.1101/2022.08.13.503850.  iGECInano NIR calcium reporter Near-infrared (NIR) fluorescent proteins promise...photochemical characteristics than previous NIR calcium reporters iGECI and NIR-GECO2G, and its small size (~1500...

  2. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...integrin antibodies available through Addgene’s repository. The collection includes 26 unique antibodies...in competition with other similar integrins, as reported by IPI and the Springer lab (Hao et al., 2024)...In this study, we, along with the Springer lab, reported the binding specificity, kinetics, and affinity... by IPI. Our integrin antibodies are the first deposit from an expanding collection of readily accessible...

  3. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...stage-specific promoter and design a UAS reporter construct, the reporter will only be expressed where and/or...UAS to examine the expression of reporter genes, or to combine reporter expression from one promoter with...

  4. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...that uniquely express selectable and/or sortable reporters in certain states of interest. For example, in...above (Sanger sequencing, qPCR, and fluorescent reporter-based) each come with their advantages and disadvantages...the most convenient strategy is the fluorescent reporter based method since it lends itself to cheap, quick...detection with flow cytometry. However, expressing reporters will consume cellular resources and therefore ...

  5. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...approaches may become even more powerful following a report that one can use linear repair templates (ie. PCR-derived... 2014). A further development of this approach reported in 2017 uses the direct injection of a CRISPR/...templates (Farboud et al., 2019).   The Mello lab also reported that modifying the 5’ modification of oligos used...mixtures (Doksin et al., 2018). The Mello lab also reported efficient editing using a co-injection marker ...

  6. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...sub-cellular localization signals, plant markers, and reporters (Engler et al., 2014). This kit is a great starter...into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This allows for the multiplexing of sgRNAs...really cool MoClo kit, we would love for you to deposit it with us to share it with the scientific community...Multi-Kingdom Golden Gate Cloning Platform.” Scientific Reports, vol. 9, no. 1, Springer Science and Business Media...

  7. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...protein analysis products. You can find plasmids deposited by Promega here.    References and resources ...ligands for dopamine D2/D3 receptors. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-78827...fluorescence-based ligand-binding assay. Scientific Reports, 6. https://doi.org/10.1038/srep25769 Grimm, J....mitochondrial dynamics in mammalian cells. Biophysics Reports, 3(4–6), 64–72. https://doi.org/10.1007/s41048-...

  8. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    ...LaFleur, who shared information about recent CRISPR deposits from Arlene Sharpe’s lab. X-Chime and Performing...Faulds, who introduced the very first antibody deposit (the Integrin collection) from the Institute for... Thirteen: Guest Contributors of 2023 8 Great Depositor Research Stories Interested in contributing to...

  10. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...telling scientists about Addgene’s repository, and explaining how to deposit and request plasmids, but I also...for: The inside scoop from scientists who have deposited plasmids at Addgene.  Learn how discoveries were...
Showing: 171 - 180 of 653 results