We narrowed to 997 results for: lars;

...shape. These AAV particles are often found with cellular exosomes, small membrane vesicles or microvesicles... - 150 nm diameter) that are secreted from the cellular membrane into the culture medium. AAV particles...inside the vesicle, which may also contain other cellular molecules such as proteins or nucleic acids. This...infect more cells (as demonstrated in different cellular environments such as retina or glioblastoma). ...to neutralizing anti-AAV antibodies. This is particularly useful for therapeutic applications in vivo ...vesicles secreted from producer cells also contain cellular macromolecules (e.g. proteins, nucleic acids) ...retina after intravitreal injection to transduce bipolar cells and photoreceptors (Wassmer et al., 2017)...

... of most molecular biology labs. Recent and rapid advances in technologies that permit large-scale creation... and PCR products are the bread and butter of molecular biology labs the world over. Scientists have traditionally...-based sequencing and confirmation of Addgene’s large and growing collection of curated plasmids from ...technique in our partnership with Addgene to sequence large numbers of plasmids. plexWell™ technology overview...sequential “pooled library prep”, in which a relatively large number of samples are subjected to a DNA-barcoding...normalization problem often arises from the fact that large numbers of individual input DNA samples (e.g. a ...measured in time and consumables, can be daunting for large numbers of samples. As mentioned above, one of the...

...Increasingly popular Optogenetics tools use light to alter cell physiology and molecular processes via...generating DNA molecular weight markers The Tan lab at Penn State - set out to “rule” Molecular Biology. Under...redshifted-emission spectrum compared to that of CyOFP1, a large stokes shift, long fluorescence lifetime, and a ...mScarlet variants have shown great performance in cellular functional imaging and in protein fusions. The...PhoCl between an enzyme and an inhibitor domain similarly allows for control of enzymatic activity. In addition...variants The toolbox of fluorescent proteins (FP) for cellular imaging is constantly expanding. Case in point...point - check out the growing list of popular plasmids in Addgene’s FP collection. Just last month, Robert...

...a pathway to inhibit to block the cellular response to a particular ligand (such as when a component of... are involved in a studied cellular behavior. This approach is particularly suitable for in vivo screens...Institutet, in Sweden. He has a particular interest in using molecular tools to study the immune system...leader of a research group at the Centrum for Molecular Medicine (CMM), Department of Medicine, Solna,...attended a great talk when I was a PhD student. In particular, one slide of the presentation made a lasting...screen and the appropriate phenotypic readout, similar mapping could be rapidly performed by targeting...signaling pathway. For example, one could study the cellular response to a cytokine known to cause the up-regulation...

... scientist wants to know if an organism has a particular gene mutation, southern blotting can be used ... is observed. Today Southern blotting has been largely replaced by real-time PCR to answer the same experimental...Southern blotting is labor intensive and requires a large amount of high-quality DNA, real-time PCR has several...next-generation sequencing techniques to answer similar experimental questions (see below). Importantly...ddNTP to a synthesis reaction along with all four regular nucleotides results in the synthesis of DNA strands...generation DNA sequencing machines (9). Today, similar technologies, sometimes referred to as sequence...discoveries (5, 8). While newer techniques have largely replaced these, these techniques still have useful...

...case of MODAL (modular overlap-directed assembly with linkers) which brings modularity to overlap-directed...deposit process in 2014 because of its gain in popularity. Gibson assembly is well known for allowing easy...insert your gene of interest into a vector with a large tag at the N-terminus, but you don’t have the tag...fairly high, so there is usually no need to screen a large amount of colonies. Excluding the time it takes ...those where the vector that you want to use is very large, has a high GC content, contains a lot of repeats...for the use of Gibson assembly combined with the popular CRISPR technology and is described in a recent ...described recently (Jiang et al. 2015), in which very large fragments of the bacterial chromosome (up to 100...

...Endogenous eNcapsidation for cellular Delivery (SEND). They demonstrate SEND as a modular system for packaging...and specify a large intervening deletion. Not only can this method precisely program large genomic deletions.... pyogenes CRISPR/Cas9 system is that it is too large to be effectively packaged into AAV particles for...  with an active nuclease.  Read the paper in Molecular Cell Find the CasMINI plasmids Efficient CRISPR...plasmids   Selective endogenous encapsidation for cellular delivery of RNAs By Mike Lacy The retroviral-like...endogenous Gag homolog, cargo RNA, and fusogen in a modular system for delivering gene editing tools into cells...(epegRNAs) protect the RNA from degradation by cellular exonucleases and broadly improve editing efficiency...

...from Christopher Grefen’s lab or the MoBiFC modular bimolecular fluorescence complementation toolkit from...available to assess protein-protein interactions. One popular approach is to fuse a protein of interest to each...Figure 1). This method is generally known as Bimolecular Fluorescence Complementation (BiFC). It can provide...proteins are split between strand 10 and 11 to yield large (1–10) and small (11) fragments. In several cases...Next, let's look at a round-up of some new and popular split-FP plasmids from our catalog to help you ...) for use with GFP(11) Multiplexed labeling of cellular proteins with split fluorescent protein tags. ...

...jumping into the discussion! Semantic Scholar Semantic Scholar is an AI-backed literature search developed...Finding research papers is not particularly hard. There are millions of them. The real challenge is finding...also set up alerts for new publications from a particular author. Saving papers Papers can be saved to ...accounts can help you find papers related to a particular topic or that were published by the lab down ...follow journals related to your field of research or larger journals that publish about a broad range of topics...Institute for Artificial Intelligence. Semantic Scholar analyzes publications and figures using machine...relevant to your search terms. Key features Semantic Scholar has several ways to focus your search results. ...

...different system (Issa et al., 2023). This can be particularly burdensome in the brain where many different...broad tropism (the ability of a virus to enter a particular cell type), but there are differences in tropism...using chemogenetic tools, where modification of cellular activity outside of your experiment site or circuit... offer the most flexibility for expression in a large number of cell types and are useful if you're going...several different systems or want to transduce a large number of different cells. Promoters such as chicken-beta-actin... Selective promoters, such as synapsin, glial fibrillary associated protein (GFAP), or tyrosine-hydroxylase...vectors in the central nervous system. Frontiers in Molecular Neuroscience, 7. https://doi.org/10.3389/fnmol...