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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 99 results for: lars;

Showing: 1 - 20 of 99 results

  1. AAV Molecular Tools

    Type
    Collection
    ...Packaging Service AAV Molecular Tools Viral Vector Packaging Service: Molecular Tools Tetracycline Transactivators...

  2. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...mTurquoise2 Dorus Gadella 26673 pEGFP-C1-Fibrillarin Nucleolus Fibrillarin EGFP Sui Huang 36207 pmTurquoise2-...proteins tagged with genes or peptides with known subcellular localization patterns. Useful for co-localization...Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization More Fluorescent Protein Resources...Cytoskeletal Junctions Secretory Pathway Background The subcellular localization of a protein is often tied to its...There are a variety of methods for determining subcellular localization. One of the most common is to use...proteins that are targeted to various organelles or subcellular structures. You should fuse your protein of interest...The cell contains a variety of organelles and subcellular structures each with their own unique function...

  3. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Pan-Gamma-protocadherin-Constant Mouse IgG2a 177504 Anti-LAR/PTPRF [N165/38R] LAR/PTPRF Human Mouse IgG2a 177505 Anti-PSD... Mouse IgG2b 225411 LAR/PTPRF (Homo sapiens) recombinant monoclonal antibody. LAR/PTPRF Human Mouse IgG2b... Anti-Pan-Neurofascin (extracellular) [A12/18R] Pan-Neurofascin (extracellular) Rat Mouse IgG2a 177439...Anti-Kv1.4 K+ channel (extracellular) [L71/5R] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 177489...Mouse IgG2a 177564 Anti-ADAM22 (extracellular) [N57/2R] ADAM22 (extracellular) Mouse Mouse IgG2a 177565 Anti-GluN2B...Anti-Kv1.4 K+ channel (extracellular) [L71/22] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 190292...-Neurofascin (extracellular) scFv [A12/18] A12/18 scFv 2t Pan-Neurofascin (extracellular) Rat Mouse 190486...

  4. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...ATP (extracellular) ecAT3.10 extracellular ATP sensor (cell surface targeted) Imaging extracellular ATP...measurement of a biomolecule while largely preserving spatial and temporal cellular processes. Note that many ...green, red or blue/green) Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA. Nat Commun...Yasushi Okada ATP (extracellular) GRAB_ATP1.0/ATP1.0mut biosensor for extracellular ATP (Constitutive ...Aug 20;524(7565):309-14. Richard Youle Osmolarity SED1 osmolarity biosensor, for expression in E. coli ...Eckhard Boles pH Imaging intracellular pH in live cells (pHRed) Imaging Intracellular pH in Live Cells with...cytosol Hypoxia triggers subcellular compartmental redox signaling in vascular smooth muscle cells. Circ...

  5. CRISPR Guide

    Type
    Collection
    ...both Cas12a and Cas3. Similarly, some Acr proteins are specific to a particular species of CRISPR enzymes... Browse Plasmids: Prime Edit Large Scale Edits CRISPR can generate large-scale genetic changes, including...Cas13a-based molecular detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection...CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening Cas9 Fusion Tools Cas9...techniques are referred to as CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeat) technologies...and adaptability, CRISPR rapidly became the most popular genome engineering approach. Figure 1: Overview...knockout or gene edit; or using Cas9 to generate large genomic deletions or inversions with two gRNA sites...

  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...PRKCG MTH Spinocerebellar ataxia 30 Frederic Mushinski 8418 pLTR PKC gamma PRKCG Spinocerebellar ataxia ...GFP CMV Spinocerebellar ataxia 27 Tobias Meyer 21204 GFP-N2-PKCgamma PRKCG GFP CMV Spinocerebellar ataxia...GFP CMV Spinocerebellar ataxia 25 Tobias Meyer 21215 GFP-C2gamma PRKCG GFP CMV Spinocerebellar ataxia ...CMV Spinocerebellar ataxia 23 Bernard Weinstein 21237 PKC gamma dNPS PRKCG HA CMV Spinocerebellar ataxia... CMV Spinocerebellar ataxia 21 Bernard Weinstein 21239 PKC gamma DN PRKCG HA CMV Spinocerebellar ataxia...CMV Spinocerebellar ataxia 15 Ryohei Yasuda 112270 CMV-PKCg-mEGFP-N1 PRKCG GFP CMV Spinocerebellar ataxia...GFP CMV Spinocerebellar Ataxia Richard Youle 176464 pVPS13D-I-EGFP VPS13D GFP CMV Spinocerebellar Ataxia...

  7. Deisseroth INTRSECT Collection

    Type
    Collection
    ...well-defined cellular sub-populations has generally been limited to single-component cellular definitions...addition to EYFP and ChR2-EYFP, a large number of additional, validated molecular payloads in the INTRSECT configuration..., Flp, and VCre to enable viral expression of molecular payloads in target populations defined by multiple...Plasmids References The development of powerful molecular reagents for biology have propelled our understanding...enabling combinatorial targeting) is a synthetic molecular targeting strategy that allows adeno-associated...multiple recombinase-expression strategies to define cellular sub-populations of interest, including dual-transgenic...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ...resistance to photobleaching have made luciferase a popular tool for scientists. Regulatory elements such as... measured by the luminescence output. The most popular types of luciferase found in Addgene's collection...but are less-than-ideal protein tags due to their large size (61 kDa and 36 kDa, respectively). And they...be lysed or the substrate must be delivered intracellularly to measure luminescence. Gaussia luciferase...blog post . Empty Backbones Browse or search for popular empty backbone plasmids into which you can clone...reference level of expression. Browse or search for popular plasmids expressing luciferase. We also offer ready-to-use...new window) : The NanoLuc® enzyme is split into Large (LgBiT; 18 kDa) and Small (SmBiT; 11 amino acids...

  9. Plan Your Experiment

    Type
    Collection
    ... Genetic Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is ...edits, including point mutations, base changes, and large insertions or deletions. For more information on...efficiencies. The template sequences can also have a large impact on editing efficiency. So while testing multiple...experiments, testing multiple pegRNA designs is particularly important for prime editing. CRISPR Intereference... . Lentiviral vectors Lentiviral vectors are a popular option for producing stable cell lines, since the...of AAV vectors is that the packaging limit (how large of a DNA sequence you can actually package into ...lipids, electroporation, or an endosomal approach. Similar to the mRNA method described above, this method...

  10. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin Nucleolus (dense fibrillar component) 87428 MYH10-mEGFP AICSDP...Nucleophosmin Nucleolus (granular component) 133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar transcription factor... generate fluorescently tagged proteins of key cellular structures in human cell lines. Depositor...CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute... generate fluorescently tagged proteins of key cellular structures in human cell lines of their choice... to create cell lines with endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute...mEGFP AICSDP-50 mEGFP Nucleophosmin Nucleolus (granular component) 109120 GJA1-mEGFP AICSDP-43 mEGFP Connexin...

  11. Bacterial Expression Systems

    Type
    Collection
    ...Plasmids Collection Empty Backbones Collection Molecular Biology Guide CRISPR Guide Bacteria are commonly...contamination and facilitates extraction. Browse our most popular plasmids for protein purification. Commonly used... purification tags compatible with Golden Gate modular cloning (MoClo) for use in E. coli . Addgene Blog...Förster resonance energy transfer (FRET) and bimolecular fluorescence complementation (BiFC) are techniques...collection of Golden Gate compatible vectors for bimolecular fluorescence complementation experiments in plants... plants. The Zinc Finger Consortium Modular Assembly Kits (Addgene #1000000005, 1000000010) contain plasmids...nucleases. Addgene Blog Introduction to FRET Bimolecular Fluorescence Complementation Makes Visualizing...

  12. Immunology Research Plasmids and Resources

    Type
    Collection
    ...with sequence similarity 3, member C GS3786, ILEI FAM3D family with sequence similarity 3, member D EF7...fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)...NR1I1 VEGFA vascular endothelial growth factor A MGC70609, MVCD1, VEGF, VEGF-A, VPF VEGFB vascular endothelial...N-RAS, RASH1 ICAM1 intercellular adhesion molecule 1 BB2, CD54, P3.58 ICAM2 intercellular adhesion molecule...The innate immune system is not specific to any particular pathogen. It includes physical barriers such ...immune response is antigen-specific and includes cellular and humoral immunity. Once an adaptive immune ...HSPA10, LAP1, MGC131511, MGC29929, NIP71 ICAM1 intercellular adhesion molecule 1 BB2, CD54, P3.58 IFI30 interferon...

  13. Promega Plasmid Collection

    Type
    Collection
    ...Promega scientists and designed to streamline molecular and cellular biology experimental workflows. The collection...NanoLuc fusions are useful for both intracellular and extracellular applications where you want minimal...structural complementation reporter system composed of a Large BiT (LgBiT; 18 kDa) subunit and a small complementary...energy transfer technique designed to measure molecular proximity in living cells. NanoBRET PPI assays...TE) assays (Link opens in a new window) have a similar premise, but examine the proximity of a chemical...

  14. p53 Pathway

    Type
    Collection
    ... for clarity and does not indicate a specific relationship. p53 Pathway Color is used for clarity and ...transcription factor and tumor suppressor activated by cellular stress. Cancer...transcription factor and tumor suppressor activated by cellular stresses like DNA damage, oxidative stress, and...damaged cells. The p53 name arose from its observed molecular weight (53 kDa) when run on an SDS-PAGE gel. Cancer...on functional p53 through oligomerization. In particular, the loss of p53’s pro-apoptotic effects is especially...only one functional copy of the p53 gene causes a large reduction in tumor suppression activity, leading...

  15. Open Enzyme Collection

    Type
    Collection
    ...Polymerase I, Large (Klenow) Fragment 165522 pOpen-K12polLF (exo-) DNA Polymerase I, Large (Klenow) Fragment...plasmids at Addgene for generating enzymes for molecular biology lab work. Depositor...for enzymes that are considered "workhorses" of molecular biology, including DNA polymerases, RNA polymerases... lab costs and overcome supply chain issues, particularly in areas of the world where enzymes are harder...Polymerase 165553 pOpen-BstpolLF Bst DNA Polymerase, Large Fragment 165562 pOpen-Pwopol Pwo DNA Polymerase ...Polymerase 165563 pOpen-BsupolLF Bsu DNA Polymerase I, Large Fragment 165570 pOpen-P45 P45 165572 pOpen-GBDpol ...

  16. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... YouTube . Abstract The prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)... and characterization. This strategy relies on cellular delivery of a pair of chimeric single guide RNAs...absence of deletion, the “deletion band” is often too large to efficiently amplify. Typically use primers at...strategy 3 . Prepare a 50 μl reaction mix: 100 ng circular pX330 vector, 1.0 μl annealed oligos (1 μM; see...identified with the desired deletion and move to larger plate or flask for growth. Validation of Biallelic...genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 ...Froger A, Hall JE. J. Vis. Exp . 2007. PubMed . Molecular cloning of PCR products. Finney M, Nisson...

  17. Neurodegeneration Research Collection

    Type
    Collection
    ...Alzheimer's disease, through large genome-wide association studies (GWAS) or other large scale studies. Researchers...these millions of patients results in billions of dollars spent each year. Unfortunately, the causes of most...with the disease and implicate a wide range of cellular processes. The vast majority of cases (90-95%)...central nervous system, peripheral nervous system, vesicular brain cells and more. New and Noteworthy: AAV....of fluorescent proteins, including Markers of Subcellular Localization, Optogenetics Plasmids, and Chemogenetics...supports the development of a diverse portfolio of biomolecular tools and emphasizes their rapid and broad dissemination...

  18. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... and tyrosine recombinases, based on sequence similarity and the amino acid residue used in the active... at once. They can mediate the integration of a large DNA payload containing an attB target site into ...the sequence between the sites is excised as a circular piece of DNA (and is not maintained). C) If the.... Using Cre or Flp as examples, here are a few popular recombinase-dependent gene expression strategies... some systems, such as Drosophila and mouse, particularly in proliferating cells. Expression may cause...controls such as Cre-only lines to account for cellular toxicity in your system. Using inducible Cre systems...Highlighted Plasmids Browse the tables below to find popular plasmids expressing your favorite site-specific...

  19. Antibody Guide

    Type
    Collection
    ...Assays Selection Validation Affinity reagents are molecular tools used in the lab to specifically and tightly...above are used to create hybridomas, which produce large amounts of a single antibody with little variation...antibody is made up of four protein chains: two large heavy chains and two small light chains. These four...In IHC, tissue sections are assayed and the extracellular matrix remains intact. The signaling molecule...enzyme. In ICC, cells have most or all of their extracellular matrix removed. Again, the signaling molecule... fluorophore colors in flow cytometry, but the largest panels used currently are in the mid-forties. When...conjugate or looking for antibodies validated for a similar assay type) and search again. After filtering, ...

  20. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus Envelope...virus (such as HIV, murine leukemia virus, and vesicular stomatitis virus G) are packaged as replication-defective...infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single round of ...particles Murine leukemia-based retroviral particles Vesicular stomatitis virus particles See Addgene's Viral...packaging plasmids. Use the search bar to find a particular viral spike protein (MERS, CoV-1, CoV-2), modification...
Showing: 1 - 20 of 99 results