We narrowed to 643 results for: ATO
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TypeBlog Post...postdoc” is, and that “PI” stood for principal investigator. In basic science (as opposed to translational...in a lab. Position Common tasks Principle investigator (PI) Runs the lab Writes a lot of grants Varying...
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Quick Guide to All Things Lentivirus
TypeBlog Post...first one and can be used routinely in a research laboratory. The risk of generating RCL is low but extra ...such as Doxycycline (Dox), the Tet-controlled transactivator (tTA, also expressed by your transfer vector... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...group focuses on gene regulation in nematode molting and spermatogenesis. His group also builds tools to ... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post... unbiased discovery. With these screens, the investigator generates a cell population where all genes ...contexts. Most of the immune cells we study are migratory, and their exact localization is important for... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...absorbed by matter, they promote electrons within atoms to a higher energy state. This process is called.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...environment? If these are pressing issues in your laboratory, then FRET experiments are a viable biophysical...and purification is a snap using thin layer chromatography (Fessenden, 2009). However, these reagents ... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post... electrophysiology data too but that’s what collaborators are for). For example, if you study neural pathways...projection-specific neuronal populations using combinatorial viral approaches. Current protocols in neuroscience... -
Selecting Your Plasmid Purification Kit
TypeBlog Post...any applications. DNA quantity is fairly self explanatory and achieved by performing different sized preps...about!) are a significant source of waste in laboratories. To help combat this, some manufacturers have... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post... as law, medical/technical writing, clinical, regulatory, product development, business development, consulting...roles in areas including: research, clinical, regulatory, project management, alliance management, business... -
Delivery Methods for Generating iPSCs
TypeBlog Post...the the ability to generate teratomas is the gold standard instead. Teratoma formation requires injecting... -
Using AAV for Neuronal Tracing
TypeBlog Post...essential to build up our current understanding of neuroanatomy and neural connectivity, providing valuable ...conditional transgenic mice, to reveal not only the anatomy of neuronal connections, but to visualize neuron... -
Fluorescent Protein Guide: Activity Regulation
TypeCollection...Plasmids Fluorescent Protein Purpose Principal Investigator Plasmids GFP GFP-dependent transcription factors... -
Zinc Finger Consortium Reagents
TypeCollection...proteins for distribution to academic and nonprofit laboratories for research and educational use. Links to additional... -
KLF Research Plasmids
TypeCollection...family of at least 17 DNA binding transcriptional regulators with diverse roles in processes such as cell ... -
Fluorescent Protein Guide
TypeCollection...signals, cells, or groups of cells with either actuators or sensors. Chemogenetics Plasmids | Guide Use... -
AAV Viral Preps
TypeCollection...Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation Monosynaptic... -
Zinc Finger Consortium: OPEN Reagents
TypeCollection...colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created... -
CRISPR Plasmids and Resources
TypeCollection...Transcriptional Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9... -
Adenovirus Plasmids
TypeCollection...double-stranded DNA viruses known to cause mild to severe respiratory disease in humans. Advantages of adenoviruses... -
Retrovirus Plasmids
TypeCollection... variant that has WRE, a post-transcriptional regulatory element that increases export to the cytoplasm...