We narrowed to 795 results for: Tec

1. Predicting Adverse Reactions to Monoclonal Antibody Drugs

   Type

   Blog Post

   ...of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 130, 128–142. https://doi.org/10.1016...

2. Improving Transduction Efficiency with Exosome AAVs

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   Blog Post

   ...association of AAV particles with membrane vesicles protects them from antibody recognition and inactivation...

3. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

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   Blog Post

   ...Bourges for editing this post and sharing her technical expertise in persistent identifiers. Additional...

4. FPbase: A new community-editable fluorescent protein database

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   ... on the Addgene Blog Fluorescence Microscopy Techniques - Which is best for me? A Practical Approach ...

5. Scientists Map the SARS-CoV-2-Human Interaction Network

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   Blog Post

   ...constructs are tagged with a 2xStrep-tag for easy detection and affinity purification. All but one are in ...

6. What Good Citizenship Can Do for Reproducibility in Science

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   Blog Post

   ...are often very subtle, and it can take a lot of detective work to figure them out. But, when they come to...

7. Creating accessible biology activities in schools with BioBits

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   Blog Post

   ...Browse our protocol videos to learn simple lab techniques Check out our molecular biology reference pages...

8. New and Upcoming Viral Vectors - September 2019

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   Blog Post

   ...of calcium dynamics. (For more details on this technology, see our blog post Overview of AAV Encoded Calcium...

9. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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   Blog Post

   ... tagged proteins, a common problem of the AID technology (Yesbolatova et al., 2019). Find the mAID tagging...

10. Viral Vectors 101: Inverted Terminal Repeats

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    Blog Post

    ..., small deletions and point mutations will go undetected by restriction digest methods.    Resources...

11. Summer SciComm Series: A PhD in Science Communication

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    Blog Post

    ... remains inaccessible, behind paywalls and in technical jargon that even scientists in neighbouring disciplines...

12. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post

    ...Assembly Blog Post Choosing a Molecular Cloning Technique Choosing Your Perfect Plasmid Backbone ...

13. Antibodies 101: Flow Cytometry Controls

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    Blog Post

    ... immunotherapies. She is currently a research technician in Arlene Sharpe's lab at Harvard Medical School...

14. Viral Vectors 101: Viral Vector Elements

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    Blog Post

    ...immune response to the virus to allow it to evade detection by the host. Of note, recombinant adenovirus is...

15. Viral Vectors 101: Biosensors

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    Blog Post

    ...as FRET-based biosensors have been engineered to detect a wide array of neurotransmitters ranging from ...

16. Viral Vectors 101: Viral Applications

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    Blog Post

    ...over a hundred years ago. Since then, medical technology has changed a lot, but viruses have stuck around...

17. Plasmids 101: Screens vs. Selections

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    Blog Post

    ...added baseline component. Alternative screening techniques While CRISPR and drugs dominate the screening...

18. Antibodies 101: Monoclonal Antibodies

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    Blog Post

    ... of Murine Monoclonal Antibodies by Hybridoma Technology. JoVE. https://doi.org/10.3791/54832  Lipman ...

19. 7 Tips to Secure a STEAM Internship This Summer

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    Blog Post

    ...Several days prior to the interview, inspect your technology, background, and lighting for a smooth performance...

20. Targeted Mutagenesis with EvolvR

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    Blog Post

    ...EvolvR-ing to targeted mutagenesis." Nature biotechnology 36.9 (2018): 819. Additional resources on the...

21. 15 Years of Addgene: The Top 15 Plasmids

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    Blog Post

    ... not contain the trademarked Tet-On(R) sold by Clontech. For additional information, see the manual provided...

22. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    ...plasmids available at Addgene Read about SARS-CoV-2 detection methods Resources on Addgene.org Find all plasmids...

23. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    Blog Post

    ...Cas domain compatibility and activity. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0453-z Additional...

24. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    Blog Post

    ... can be especially important for base editing techniques, where Cas9 must be directed to a very specific...

25. Hot Plasmids - May 2022

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    Blog Post

    ...Kaufman, et al, 2022.    This new barcoding technology would allow researchers to avoid the off-target...

26. How to Keep a Lab Notebook for Bioinformatic Analyses

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    Blog Post

    ... in the same directory where it was run. This technique leaves a trail that further helps you keep track...

27. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    Blog Post

    ...a gBlocks® Gene Fragment from Integrated DNA technologies. Generally speaking, anyone using CRISPR/Cas9...

28. Plasmids 101: Yeast Vectors

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    Blog Post

    ...proteins yet they still share many of the same technical advantages that come with working with prokaryotes...

29. Viral Vectors 101: An Introduction to AAV

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    Blog Post

    ...Towards Gene Therapy For Duchenne Muscular Dystrophy. InTech. doi: 10.5772/53023 Veldwijk, Marlon R., et al....

30. All in a Twist: dsRNA

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    ... RNA-induced silencing complexes (RISCs), which detect dsRNA in the cytoplasm and use it as a guide to...

31. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    ...first iteration of these workshops took place at Caltech in Jan 2018 and involved presentations and a handout...

32. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post

    ...strong in this one. After all, the system built to protect us now seems to attack us by releasing mitogenic...

33. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    Blog Post

    ...expression at any copy number in bacteria." Nature biotechnology 36.4 (2018): 352. PubMed PMID: 29553576. 2. ...

34. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post

    ...methods such as patch-clamp electrophysiology, a technique that’s used to measure electric current of ion...

35. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post

    ...signaling pathways G protein-coupled receptors (GPCRs) detect molecules outside of the cell and translate those...

36. Seven Tips for Using LinkedIn as a Scientist

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    Blog Post

    ...examples of concrete accomplishments in both the technical and “plus skills” areas. Include a professional...

37. Antibodies 101: Affinity Reagents

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    Blog Post

    ...sensitive drug delivery systems. Elsevier Science & Technology Books. Bates A, Power CA. David vs. Goliath: ...

38. Human Germline Editing Using CRISPR

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    Blog Post

    ...achieved any end goal not available with current techniques like PGD.  Reference Ma, Hong, et al. "Correction...

39. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    Blog Post

    ...The first time through, just get an idea of the techniques used in the research, but wait to sort out any...

40. Career Coaching for Scientists: Why and Where Do I Find One?

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    ...is a proven coach who helps people in science and tech get jobs. She is also a very popular speaker and...