We narrowed to 990 results for: Abo

... your strengths in your resume and don't be shy about asking for help. The worst thing that can happen...

... absorbance of water, which absorbs wavelengths above 900 nm or so. In other words, wavelengths between...

...Biotechnology. 2013 Dec 23. (Article). Read more about CRISPRs: Using CRISPR/Cas to Edit Disease Out of...

...Additional Resources on the Addgene Blog Learn more about in vivo imaging tools The luciferase reporter gene...

...Additional Resources on the Addgene Blog Learn about Homology Directed Repair 3 Tips to Improve HDR Efficiency...

...Avoid Fluorescent Protein Oligomerization Learn about Small Molecule Fluorphores Resources on Addgene.org...

... Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now? Academic...

...to purify DNA without a kit Read our blog posts about plasmid cloning Browse our Plasmids 101 blog posts...

...Brian O'Neill  The François St-Pierre lab and collaborators have successfully developed an improved voltage...

...CRISPR library from Alec Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene.  CRISPR-Act3.0, ...

...isolate a protein, there must be a special feature about that protein that no other protein in the expression...

...intern coordinator shortly after hearing her talk about Addgene’s internship opportunity taking place next...

...the commercial "Nanobody" name.  We’ve talked about sdAbs a number of times on this blog over the years...

...of postdocs focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with...

...type of edits can be made in the contexts outlined above. Layers of control have also been added to the CRISPR...

...of DNA that can move around in a genome. In a laboratory setting, transposons can be used to both introduce...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here. Listen...

...in mind that Taq polymerase has an error rate of about 1 in 3,500 bases. Typically polymerases with proofreading...

...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

...Additional resources on the Addgene blog Learn about visualizing protein turnover Download the fluorescent...

...Additional resources on the Addgene blog Learn more about induced pluripotent stems cells (iPSCS) and reprogramming...

...measurement and it provides limited information about what materials or organelles are being targeted ...

... research ranged from wildlife conservation to abortion law to poetry. A research discipline in its own...

...molecular biology and viral vector protocols Learn about the common lab E. coli strains Find tips for viral...

...Browse all COVID-19 related blog posts Learn more about pseudotyping Resources on Addgene.org Find plasmids...

...variations in the spike protein, teaching us more about viral evolution and antibody binding. Figure...

...I often wonder about the gut-brain axis (admittedly for self-serving reasons – I want to understand my...

... currently assumed that the cells came from an aborted fetus. HEKs have an unusual ploidy and are considered...

...speaking with us! Additional Resources Find out more about the Rare Genomics Institute and the BeHeard Award...

...spring of 2015 and since that time have received about 3 project proposals per month.  We are currently...

... a protocol has been established) may be less laborious than viral purification, and it does not raise...

...diluting - if you end up concentrating your AAV above your desired titer and want to dilute it, err on...

... Additional Resources on the Addgene Blog Read About Other Hot Plasmid Technologies Read Other Synthetic...

...Additional Resources the Addgene Blog Learn More about SunTag Catch up on Your GFP Background Choose the...

... use the Gal4/UAS system, and you can read more about them in review articles (2–4). You can also take...

...return the sample back to the original pH. What about putting it back in the freezer? We then tested whether...

... with plasmids deposited at Addgene – typically about four times as many as papers without plasmids deposited...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...

...the Addgene blog Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR ...

...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

... mitochondrial DNA disorders.” This document is about the popular technique incorrectly known as “three-parent...

...therapeutic.” There are many fundamental questions about CRISPR/Cas biology still left to answer as well,...

...Additional Resources on the Addgene Blog Learn about other Fluorescent Biosensors A Practical Approach...

... minutes of FUS.  Similar constructs to the one above can be made for expressing your favorite T-cell ...

...received his PhD in biophysics from UCSF in the laboratory of Ronald Vale, after which he was a fellow at...

...1kb ladder (lane 1) that has bands ranging from about 500bp to 10kb, with the 3.0kb fragment having increased...

...within 3-5 days and resistant colonies appear in about 10-14 days depending on how quickly your cells divide...

...light-regulated two component systems designed by the Tabor lab. Which inducible promoter is right for me? Here...

...our CRISPR 101 blog posts Find more information about Cas proteins Download the CRISPR 101 eBook Resources...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...