We narrowed to 1,033 results for: CAT;

...delegating well, but it does get easier over time! Communicate often and clearly As a manager you need to clearly...it is the best way to avoid mistakes due to miscommunication and to detect trouble. I’m not comfortable...

...results. Most cell culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red))...Anything between orange and light orange usually indicates that the cells have grown enough, are making their...

...of LSFM microscopes (3,4). After this initial application, Stelzer’s group described the single-plane or...brightness with increasing depth caused by light scattering and absorption.  Finally, the photomultipliers...

...search to check for expected ORFs, mutations or truncations. A primary advantage of blastx is that you do...other labs speed up their plasmid and cloning verification steps and free up more time for using your plasmids...

... creates a template that’s suitable for PCR amplification using pre-designed primers. By using this library...morphologic studies), as well as translational applications (mechanistic studies and gene therapy treatments...

... check out Addgene’s blog, eBooks, and other educational resources to help you get started!) Are there...something. It can be intimidating to ask for clarification at first, but in the end everyone wins. Your...

...The glycoprotein will be incorporated into the replicating rabies virus envelope, travel retrogradely, and... Pseudotypes: Precious Tools to Improve Gene Modification of Hematopoietic Cells for Research and Gene...

... conferences are held every year in different locations all over the world. But simply attending the conference...work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great blog ...

...involves a few key features that can be difficult to replicate in other work settings: The sense of being the... might include collaborating, managing, and communicating. After years of focusing on my technical skills...

...ribosome may therefore stall at inappropriate locations or fail to make it through the entire transcript...generally segregate with the cell pellet during purification procedures. Even if you produce a large amount...

...differences due to in silico variations and lack of verification. To address this issue, the QC team developed...Nanopore sequencing technology, bioinformatics and applications. Nature Biotechnology, 39(11), 1348–1365. https...

...have made great strides to map brain regions and allocate specific functions to them. Electrical recordings...long-lasting antidepressant effects." Nature communications 10.1 (2019): 223. PubMed PMID: 30644390. PubMed...

... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging.....org Find Cas9 Activators Find Cas9 Repressors Catch Up on Your CRISPR Background with Our CRISPR Guide...

...allows the detection of molecules' interactions, modifications or dissociations in situ. Used since the mid...14. Zimmermann T,  Terjung S. FRET Basics and Applications: An EAMNET Teaching Module http://www.embl.de...

... throughout their lifetime. Thus any genetic modification, such as expression of fluorescent protein or...studying somatic mutations and cancer." Nature Education 1.1 (2008): 183. Zuber, Johannes, et al. "Mouse...

... allele To design a knock-in allele at a given location, you’ll need to design only a single guide and... out our CRISPR Guide Find Plasmids for Cas9 Purification Find CRISPR Plasmids for Mammalian Expression...

...reporter, such as GFP or mCherry, through genetic modification. As your protein of interest will only occur...excitation and emission. The visible color spectrum is located in a range of wavelengths of about 380–700 nm. ...

... required for a growing organism to make the complicated transition from a clump of cells to a fully differentiated...relatively little tissue absorption, weak tissue scattering and small amounts of tissue auto-fluorescence...

...the user. This approach is useful for several applications as will be discussed below. The software could...contain multiple gRNAs targeting all genes in the indicated species and were suggested to be the best gRNAs...

...joined Cell Press to pursue a career in science communication and publishing after completing her postdoctoral...PMC4096605. 30. Lamba, D.A., et al., Generation, purification and transplantation of photoreceptors derived...

... Western blot and share tips for this tricky application.    You can check out all of our protocols ...

...place in our hearts: our first two offices were located in Kendall Square in Cambridge, MA. We hope you...

...suggestaplasmid, the name of the plasmid and a publication where we can find the plasmid. While we cannot...

...On the ninth day of CRISPR, Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  ...

... information on the basics of viral vectors, applications, and some new resources available from Addgene...

...Additional Resources from the Addgene Blog Find education resources Use our Plasmids 101 eBook to teach ...

...Finally, our team is rounded out with our science communication specialist (you'll know me by my Tweeting — ...

...important details that you won’t find in the final publication and we have already collected over 100 entries...

...Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits We are very grateful for each and...

...Sapozhnikov tells us how to use dCas9 (with no modifications!) as a CRISPR/dCas9-based tool for DNA demethylation...

... to any molecular biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors...

...create a stack of wrapped gifts using life science catalog pages. Make your WFH space feel a little more festive...

...blog posts on fluorescent proteins Check out educational resources on the blog Resources on Addgene.org...

...right). Once on the “Filters” page, select the category “LinkOut” and search for “Addgene”. Then check...

...materials (check out our new Viral Service), and educational outreach. Many thanks to Alex Chavez for taking...

...tropism* (min. 300 dpi, PNG format) At least two replicates of your experiment* Functional data, if the report...

...vectors in the Addgene repository  Name Serotype Category Depositor  Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE...

...streaking to a single colony to DNA extraction and quantification so that you can focus on what you want to do...

...wearestemsquad #festivefun #phdlife #phdtips #sciencecommunication #sciencedaily #instascience #ladies #research...

...vectors in the Addgene repository  Name Serotype Category Depositor  Notes AAV-CAG-jGCaMP8s-WPRE AAV1 ...

...health research, the HEAD outreach program, and educating the public about dietary health. 40:17 - 44:14...

...concluded, Addgene and Seeding Labs will be opening applications for a second round of plasmid awards on June...

...Doench (Guest Blogger) Plasmids 101: Origin of Replication By Kendall Morgan Save Time and Money by Making...

...monomeric Cas9’s. Read the paper in Nature Communications Find the Cas9-Cas9 chimera plasmids A modular...

...office – previously at 1 Kendall Square, but newly relocated to 75 Sidney Street. Then the plasmids travel ...

...of dsDNA and RNA in trans. Fluorescent signal indicates the presence of this viral sequence in the assay...

...as links to the Zhang Lab's Nature Protocols Publication describing them here. References 1.Ran, F. Ann...

...says the new tool might even find therapeutic application in addition to their use in basic biology, for...

...right path. The frequent Zoom meetings helped me communicate my ideas. I was able to express my visions for...

...pmSyn1-EBFP-Cre Of course there are also more complicated ways to use this particular AAV. For instance...