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We narrowed to 1,030 results for: CAT;

Showing: 851 - 900 of 1030 results

  1. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...delegating well, but it does get easier over time! Communicate often and clearly As a manager you need to clearly...it is the best way to avoid mistakes due to miscommunication and to detect trouble. I’m not comfortable...

  2. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...of LSFM microscopes (3,4). After this initial application, Stelzer’s group described the single-plane or...brightness with increasing depth caused by light scattering and absorption.  Finally, the photomultipliers...

  3. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...search to check for expected ORFs, mutations or truncations. A primary advantage of blastx is that you do...other labs speed up their plasmid and cloning verification steps and free up more time for using your plasmids...

  4. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ... creates a template that’s suitable for PCR amplification using pre-designed primers. By using this library...morphologic studies), as well as translational applications (mechanistic studies and gene therapy treatments...

  5. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...ribosome may therefore stall at inappropriate locations or fail to make it through the entire transcript...generally segregate with the cell pellet during purification procedures. Even if you produce a large amount...

  6. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...have made great strides to map brain regions and allocate specific functions to them. Electrical recordings...long-lasting antidepressant effects." Nature communications 10.1 (2019): 223. PubMed PMID: 30644390. PubMed...

  7. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...differences due to in silico variations and lack of verification. To address this issue, the QC team developed...Nanopore sequencing technology, bioinformatics and applications. Nature Biotechnology, 39(11), 1348–1365. https...

  8. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...The glycoprotein will be incorporated into the replicating rabies virus envelope, travel retrogradely, and... Pseudotypes: Precious Tools to Improve Gene Modification of Hematopoietic Cells for Research and Gene...

  9. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... conferences are held every year in different locations all over the world. But simply attending the conference...work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great blog ...

  10. How to Be an Excellent Trainee

    Type
    Blog Post
    ... check out Addgene’s blog, eBooks, and other educational resources to help you get started!) Are there...something. It can be intimidating to ask for clarification at first, but in the end everyone wins. Your...

  11. Lab to Office Culture Shock

    Type
    Blog Post
    ...involves a few key features that can be difficult to replicate in other work settings: The sense of being the... might include collaborating, managing, and communicating. After years of focusing on my technical skills...

  12. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging.....org Find Cas9 Activators Find Cas9 Repressors Catch Up on Your CRISPR Background with Our CRISPR Guide...

  13. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...allows the detection of molecules' interactions, modifications or dissociations in situ. Used since the mid...14. Zimmermann T,  Terjung S. FRET Basics and Applications: An EAMNET Teaching Module http://www.embl.de...

  14. Five Popular Model Organisms

    Type
    Blog Post
    ... throughout their lifetime. Thus any genetic modification, such as expression of fluorescent protein or...studying somatic mutations and cancer." Nature Education 1.1 (2008): 183. Zuber, Johannes, et al. "Mouse...

  16. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...reporter, such as GFP or mCherry, through genetic modification. As your protein of interest will only occur...excitation and emission. The visible color spectrum is located in a range of wavelengths of about 380–700 nm. ...

  18. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...the user. This approach is useful for several applications as will be discussed below. The software could...contain multiple gRNAs targeting all genes in the indicated species and were suggested to be the best gRNAs...

  23. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...On the ninth day of CRISPR, Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  ...

  24. We’re Going Viral This Week!

    Type
    Blog Post
    ... information on the basics of viral vectors, applications, and some new resources available from Addgene...

  26. Addgene at SfN23!

    Type
    Blog Post
    ...Finally, our team is rounded out with our science communication specialist (you'll know me by my Tweeting — ...

  28. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...Sapozhnikov tells us how to use dCas9 (with no modifications!) as a CRISPR/dCas9-based tool for DNA demethylation...

  30. Call for Guest Bloggers

    Type
    Blog Post
    ... to any molecular biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors...

  36. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...vectors in the Addgene repository  Name Serotype Category Depositor  Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE...

  37. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...tropism* (min. 300 dpi, PNG format) At least two replicates of your experiment* Functional data, if the report...

  38. New Viral Vectors - March 2024

    Type
    Blog Post
    ...vectors in the Addgene repository  Name Serotype Category Depositor  Notes AAV-CAG-jGCaMP8s-WPRE AAV1 ...

  39. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...wearestemsquad #festivefun #phdlife #phdtips #sciencecommunication #sciencedaily #instascience #ladies #research...
Showing: 851 - 900 of 1030 results