... annual #DeckTheLab contest. The bar is set very high from last year’s impressive entries, but we have...

... to me… Antibodies developed by IPI!   🎶On the eighth day of Antibody, Addgene gave to me…  A recombinant...

...significantly higher density than water, then you may need to fill the blank based on weight instead of ..., which is generated by spinning the sample at a high speed. Being able to separate solids from a liquid...Centrifuges sit on the floor and can be about bench height. They often spin at similar speeds to their smaller...larger containers, but they can also rotate at much higher speeds and are used for more specialized techniques...distributed evenly around the center (see image to right). Even distribution ensures that the centrifuge ...

...Segment on mCyRFP1 Hot Plasmid 4: mScarlet - a new, bright, and monmeric red fluorescent protein.  Listen ...

...will often get higher transformation efficiencies with less DNA, especially when using highly competent cells...transformation efficiencies upon thawing. For the highest transformation efficiency, we recommend that you... for the purposes of storage and amplification. Higher efficiency cells are more important if you will...won't grow in colonies. Incubate plates at 37°C overnight. Tips and FAQ How can I save time when carrying...reduce the efficiency of the transformation, so when higher efficiency is needed follow the complete protocol...

...editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR, Addgene gave to me: the PEDAR strategy...

...Goedhart  Created in collaboration with Addgene writers High Complexity Golden Gate Assembly with NEB's DADExperts...

...annual #Deckthelab contest. The bar was set very high from last year’s impressive entries, but our community...

...with you.  Top 5 science comics:   1)   “Piled Higher and Deeper” by Jorge Cham   2)   “Boxplot” by Maki...

...in 8,900 institutions across 111 countries. Just eight days into 2024, we've already shipped 2,078 items...

...journey. And if you have additional resources that might be helpful, please reach out to me by email.  About...

...topic page Read Joachim's Series on Choosing the B(right)est Fluorescent Protein Plasmids 101: Visualizing...

...organized by graduate student researchers. That’s right, #ActualLivingScientists, eager to communicate their...

...Use a "Universal" SYBR master mix which contains a high-quality DNA polymerase and a blend of dTTP/dUTP ... use plasmid #59462 from Addgene. The values highlighted below in red were calculated using this plasmid...of Addgene plasmid #59462 : 1.07 μg/μL Molecular Weight: 6208 bp x 650 dalton/bp or (g/mole)/bp = 4.03 ...Dilution 8 20 uL Dil. 7 80 uL 5X 3125000X Dilutions highlighted in green are the ones loaded onto the qPCR plate...

...plasmids at Addgene, along with other tools like high resolution Ras sensors and crosslinked peptides ...

...the Expression and Insert Species filters on the right-hand side of the search page to narrow your keyword...

...and career goals, or to answer any questions you might have about Addgene. The page is divided into three...

...acrylamide when immunoblotting high molecular weight proteins and higher percentage acrylamide when immunoblotting...protocol may need to be adjusted to accommodate slight differences between products. Addgene does not ...immunoblotting low molecular weight proteins. This protocol uses a dry transfer device but can be adapted...typically between 1–10 μg/mL. Incubate the membrane overnight in primary antibody at 4 °C on a rocking platform...

... University with the backing of a prestigious Fulbright Fellowship. Dr. Kwapata now works as a lecturer...

...finally warming up in Boston and that means summer is right around the corner. Here at Addgene, we’re always...

...winner will be determined by popular vote. That’s right - you get to choose the new name! Additionally, ...

...of the contest is our traditional approach. You might decorate your lab in pipette tip snowflakes, hang...

...fluorescent protein family that give off color in ambient light. Expressing these chromoproteins in E. coli would...

...plasmids at specific molar ratios. To get these ratios right, you need to do a few calculations. This calculator...

... about Crystal’s exciting career path, to gain insight into industry research, and to get advice on how...

...shape Addgene's future. Listen to this episode for insights into the process of getting an MD PhD, the inside...

...back on some fascinating Addgene history that you might not have known about. Here's the schedule for the...

...plasmids shipped. Reaching one million plasmids shared might have you wondering how many plasmids leave Addgene...

...open to industry scientists. This collection is highly diverse in terms of target organisms (from bacteria...

... school for microbiology, and I ended up going straight to law school. This was in the early 80s, and ...

...interneurons. Take a look to see if these new preps might enhance your research!   Table 1: New ready-to-use...

...the labs they’re granted to, as well as shine a light on some of the great minds working in developing...

...for detecting genome edits, to discover why you might want to use Reddit to discuss your science and more...

...subtract the weight of the empty tube from the weight of the tube with the gel fragment. The weight of the ...gel in a labeled microfuge tube. Using a scale, weigh the tube with the gel fragment after zeroing the... the gel percentage to get better separation. A higher percentage agarose gel will help resolve smaller...

...sample Using a Light Microscope Learn about the parts of a light microscope and its use Weighing Reagents Learn...applications, with videos for select protocols in the right-hand column. You can find even more video content... Learn how to weigh laboratory materials on a balance Basic Molecular Biology Name Description (Link opens...

...community through sharing science and distributing high-quality materials and information. As a scientist...

...lentiCRISPR v2 as the backbone for their many gRNAs. The high titer lentivirus production afforded by lentiCRISPR...

... Find job listings at Addgene Get more career insight from Addgenies ...

...One Pot, One Step, Precision Cloning Method with High Throughput Capability." PLoS ONE. 3(11): e3647 (...

...little more certain that I was being led down the right path. The frequent Zoom meetings helped me communicate...

...contributions to key research papers are already inspiring higher standards in antibody use (Ayoubi et al., 2024;...

...controller Hazardous waste container Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5mL...sample of possible dilutions. You may want to try higher/lower dilutions depending on your downstream applications...transduced with lower dilutions of the virus will have higher levels of expression. Consider expanding populations...population may drop. This is because cells that express high levels of the transgene may have reduced growth ...

...expression system. If you find that your protein might not fold well, you should screen a few different...

...microscope, you too can become an artist. Scientists moonlighting as artists can compete in the Nikon Small World...

...looking at their performance in categories such as brightness, stability, and disruption of the tagged protein...

...counter Reagents Polyclonal stable cell pool DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5...for one 96-well plate. Allow the cells to grow overnight. Replace the medium on the cells and collect the...conditioned medium, it will be more accurate if a higher volume of homogenized cell solution is transferred... Western blotting to screen for lines with the highest or lowest transgene expression ( Figure 2 ). Sample...

...be targeted with optical stimuli. The restricted light exposure to a specific area in combination with ...

... Since the signal level provided by SunTag is so high, cells may be imaged under lower illumination settings...

...consider. Antibiotic selection Selection is straightforward in cases where the inserted DNA includes an...

...document may sound like something out of your nightmares, shared ownership of materials produced from ...