We narrowed to 933 results for: KIN

...We’re always looking for ways to improve our shipment processes. After reading a publication describing...

... argument that Addgene is achieving its goal of making plasmid-based science more accessible…and accruing...

...predecessor CRISPRainbow, CRISPR-Sirius is brighter making it suitable for observing low-copy genomic loci...

...contamination based on the presence of other bands or streaking. Unlike absorbance based methods, this method ...

...generate multiple gRNAs for nCas9-BE, or any other kinds of CRISPR tools, without compromising their strategic...

...changes) the original identifier loses its meaning, making it difficult or impossible for researchers to track...

...with research! It is always important to know what kind of reagents you work with in experiments. Hence,...

...roles leads to more ideas, initiative and creative thinking. The team members will be more invested in the...

...infrared channels used for more abundant proteins or marking compartments. I hope this sheds some light on multicolor...

...milestone in a scientist's career is mediated by some kind of communication to another person.  In an interview...

...compelling narrative that is positive, and forward-looking. Embrace it, and let your personality leap out ...

...perfect clone that contains the plasmid you’re looking for out of the many cells that don’t. One way to...

...also transduce dividing and non-dividing cells, making it conducive to targeting all cell types. Altogether...

...specific transcription response element (TRE) or kinase translocation reporters (KTRs). Find visual barcode...

...advancement could be especially exciting for scientists working to visualize multiprotein complexes.  Fig...

..., including live-cell imaging, single-molecule tracking, immunolabeling of fixed cells, Western blotting...

...read-outs of Cas9/gRNA efficiency. So if you are looking to identify the most effective gRNA and enrich ...

...adenine base editors can correct all four of these kinds of point mutations, in theory converting the disease-associated...

...cold-tolerant E. coli! Additionally, we’re also working towards widening the applicability of our strategy...

...activate the DREADD, CNO is delivered either through drinking water or intravenously. Tips for using pAAV-hSyn-DIO-hM4D...

...purposes or for diagnostics, we suggest double checking to make sure your results will not be affected...

...BRD2/3/4, CDK9, TRIM24, FLT3, BTK, and ALK) by linking small molecules that bind these target proteins...

...bioelectrical signals affect pattern memory and decision-making in somatic cell networks. “Developmental bioelectricity...

...verify that the sequence of the plasmid you are working with is correct. It's also best to use 1-2 ug of...

... experiment! As a starting point, we recommend checking the literature and our AAV data hub to see if ...

...1321–1325. Janknecht R, de Martynoff G, Lou J, Hipskind RA, Nordheim A, Stunnenberg HG. Rapid and efficient...

... good deal of it. Researchers are continuously looking for ways to improve direct methods and avoid the...

...some regions of the protein may be masked by crosslinking (ChIP) or fixation (IHC). Because of their increased...

...Göttingen in Germany. I attended this symposium seeking inspiration to resolve the technical hurtle of ...

...rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live cells. However...

...protein (CcdB) that acts as a DNA gyrase poison, locking up DNA gyrase with broken double stranded DNA and...

... has revolutionized how we decode the genome by making it easy to create specific genetic perturbations...

... will depend on the particular plasmid you are working with, but, in most cases at Addgene, we check what...

...types, during certain stages of development, or by making the Cre inducible (such as with tamoxifen or doxycycline...

...ensure the entire team is using the same rubric and making the same decisions when analyzing the wide variety...

...of conjugation, and these are encoded by certain kinds of plasmids — more on that in a minute! Fun fact...

...tips and tricks for overcoming these hurdles. Talking 'bout my (lentiviral) generation The first step...

...Nanoblades? They’re easily produced in a BSL2 lab, making them a versatile and accessible genome editing ...

...associate, self-excise, and covalently ligate their flanking peptides together (Wang et al., 2018).” With the...

...References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application of Murine Monoclonal Antibody...

...HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents...

...mean it’s suitable for your experiment. If you’re looking for more pan-genome PAM flexibility, you’ll want...

...pipelines (viral), and researchers are currently working on giving this treatment a CRISPR spin. CRISPR ...

...process. Figure 1: Begin the deposit process by clicking “Submit Online Deposit”or “Download Deposit Spreadsheet...

...eBook to get all the background you need to start working with plasmids! What are termination and polyadenylation...

...were the only realistic options for scientists working in regenerative medicine, but the longing for a...

...antibodies and pharmaceuticals, specifically interleukins. Yeast expression systems Yeast are a great ...

...gene in the recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients...

...relative quantification - that is, since you’re looking at the amount of DNA in your sample relative to...

...membrane-bound spike protein, in a remarkable 12 working days. While the lab used this method for COVID-...