We narrowed to 929 results for: KIN

1. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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   ...contamination based on the presence of other bands or streaking. Unlike absorbance based methods, this method ...

2. Visualizing Genomic Loci with CRISPR-Sirius

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   ...predecessor CRISPRainbow, CRISPR-Sirius is brighter making it suitable for observing low-copy genomic loci...

3. DAP arrays for multiplex gene editing

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   ...generate multiple gRNAs for nCas9-BE, or any other kinds of CRISPR tools, without compromising their strategic...

4. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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   ...We’re always looking for ways to improve our shipment processes. After reading a publication describing...

5. Addgene Depositors Get More Citations

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   ... argument that Addgene is achieving its goal of making plasmid-based science more accessible…and accruing...

6. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

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   ...changes) the original identifier loses its meaning, making it difficult or impossible for researchers to track...

7. 5 Great Apps for Lab Life

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   ...with research! It is always important to know what kind of reagents you work with in experiments. Hence,...

8. Management for Scientists: Delegating is Key

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   ...roles leads to more ideas, initiative and creative thinking. The team members will be more invested in the...

9. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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   ...infrared channels used for more abundant proteins or marking compartments. I hope this sheds some light on multicolor...

10. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    ...perfect clone that contains the plasmid you’re looking for out of the many cells that don’t. One way to...

11. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    ...read-outs of Cas9/gRNA efficiency. So if you are looking to identify the most effective gRNA and enrich ...

12. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    ...adenine base editors can correct all four of these kinds of point mutations, in theory converting the disease-associated...

13. Communicating Your Science With Help From ComSciCon

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    ...milestone in a scientist's career is mediated by some kind of communication to another person.  In an interview...

14. Hot Plasmids - May 2022

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    ...specific transcription response element (TRE) or kinase translocation reporters (KTRs). Find visual barcode...

15. Building and Validating Recombinant Antibodies: A Case Study

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    ...advancement could be especially exciting for scientists working to visualize multiprotein complexes.  Fig...

16. Hot Plasmids - February 2022

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    ..., including live-cell imaging, single-molecule tracking, immunolabeling of fixed cells, Western blotting...

17. Viral Vectors 101: Types of viruses

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    ...also transduce dividing and non-dividing cells, making it conducive to targeting all cell types. Altogether...

18. 7 Tips to Secure a STEAM Internship This Summer

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    ...compelling narrative that is positive, and forward-looking. Embrace it, and let your personality leap out ...

19. Developing a cold tolerant E. coli using specialized chaperones

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    ...cold-tolerant E. coli! Additionally, we’re also working towards widening the applicability of our strategy...

20. Top Requested Lentivirus and AAV of 2016

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    ...activate the DREADD, CNO is delivered either through drinking water or intravenously. Tips for using pAAV-hSyn-DIO-hM4D...

21. Plasmids 101: Methylation and Restriction Enzymes

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    ...purposes or for diagnostics, we suggest double checking to make sure your results will not be affected...

22. dTAG - You're it!

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    ...BRD2/3/4, CDK9, TRIM24, FLT3, BTK, and ALK) by linking small molecules that bind these target proteins...

23. Visualizing Protein Turnover In Situ

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    ...Göttingen in Germany. I attended this symposium seeking inspiration to resolve the technical hurtle of ...

24. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    ...bioelectrical signals affect pattern memory and decision-making in somatic cell networks. “Developmental bioelectricity...

25. Troubleshooting Your Plasmid Cloning Experiment

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    ...verify that the sequence of the plasmid you are working with is correct. It's also best to use 1-2 ug of...

26. Antibodies 101: Secondary Antibodies

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    ... good deal of it. Researchers are continuously looking for ways to improve direct methods and avoid the...

27. Antibodies 101: Polyclonal Antibodies

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    ...some regions of the protein may be masked by crosslinking (ChIP) or fixation (IHC). Because of their increased...

28. CRISPRainbow and Genome Visualization

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    ...rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live cells. However...

29. Viral Vectors 101: An Introduction to AAV

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    ... experiment! As a starting point, we recommend checking the literature and our AAV data hub to see if ...

30. To Each HIS Own

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    ...1321–1325. Janknecht R, de Martynoff G, Lou J, Hipskind RA, Nordheim A, Stunnenberg HG. Rapid and efficient...

31. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ...protein (CcdB) that acts as a DNA gyrase poison, locking up DNA gyrase with broken double stranded DNA and...

32. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    ... has revolutionized how we decode the genome by making it easy to create specific genetic perturbations...

33. Addgene's Tips for Plasmid Quality Control

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    ... will depend on the particular plasmid you are working with, but, in most cases at Addgene, we check what...

34. Plasmids 101: Cre-lox

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    ...types, during certain stages of development, or by making the Cre inducible (such as with tamoxifen or doxycycline...

35. Overcoming the Challenges of Lentiviral Production

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    ...tips and tricks for overcoming these hurdles. Talking 'bout my (lentiviral) generation The first step...

36. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    ...Nanoblades? They’re easily produced in a BSL2 lab, making them a versatile and accessible genome editing ...

37. Open Resources and Plasmid Tools For Studying C. elegans

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    ...associate, self-excise, and covalently ligate their flanking peptides together (Wang et al., 2018).” With the...

38. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    ...HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents...

39. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ...process. Figure 1: Begin the deposit process by clicking “Submit Online Deposit”or “Download Deposit Spreadsheet...

40. A Look at Addgene's QC Process

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    ...ensure the entire team is using the same rubric and making the same decisions when analyzing the wide variety...

41. Plasmids 101: The Wide World of Natural Plasmids

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    ...of conjugation, and these are encoded by certain kinds of plasmids — more on that in a minute! Fun fact...

42. CRISPR in the Clinic

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    ...pipelines (viral), and researchers are currently working on giving this treatment a CRISPR spin. CRISPR ...

43. Progress Towards a PAM-Free CRISPR

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    ...mean it’s suitable for your experiment. If you’re looking for more pan-genome PAM flexibility, you’ll want...

44. Antibodies 101: Choosing the Right Isotype

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    ...References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application of Murine Monoclonal Antibody...

45. Plasmids 101: Terminators and PolyA signals

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    ...eBook to get all the background you need to start working with plasmids! What are termination and polyadenylation...

46. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ...membrane-bound spike protein, in a remarkable 12 working days. While the lab used this method for COVID-...

47. Plasmids 101: Protein Expression

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    ...antibodies and pharmaceuticals, specifically interleukins. Yeast expression systems Yeast are a great ...

48. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    ...gene in the recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients...

49. PCR Applications for Copy Number Variation Assays

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    ...relative quantification - that is, since you’re looking at the amount of DNA in your sample relative to...

50. Plasmids 101: Degron Tags

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    ...round of evolution (AID-2) to reduce some of the leakiness associated with the system, but the general concept...