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We narrowed to 1,025 results for: URE

Showing: 851 - 900 of 1025 results

  1. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ... introduce genes into an organism’s genome (see figure) and to disrupt endogenous genes at the site of...antibiotic-resistance genes conducted in HeLa cells). In their Nature Genetics paper, the lab showed that efficiencies...

  2. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...mechanisms for feedback (more on this below). Make sure to give positive feedback when it is merited. Surprisingly...unnecessarily big issue for you and for the employee. Make sure to focus on the action, not the person - intent ...

  5. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...new. Science involves success and, more often, failure. It’s useful for non-scientists to understand not...science and society 6)   Consider participating in a future SciFund Challenge. While the goal is ostensibly...

  6. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ... tool. They can search the database by certain features like serotype, promoter, or tool type and each...use these questions as a guide for designing your future injection experiments. Have your data or lab notebook...

  7. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ... been difficult to modify in the past.   Figure 1: Identification and enrichment use of XMAS-TREE...splice acceptor and donor sites, and introducing premature stop codons.    New from the viral service By...

  11. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...through biotin-avidin binding could provide a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand...map and cartoon below). Including biotin in your culture and inducing protein expression with IPTG results...

  12. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...questions help us expand and improve our resources for future users. We’re excited to be distributing new types...fulfilled more than 140 orders for virus! We’ll be sure to keep you updated on our progress and would love...

  13. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine...Looger’s lab at Janelia for neuroscientists to measure glutamate levels. iGluSnFR.A184S, a variant with...

  15. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...interspaced with linkers that can fold into a secondary structure that can bind specific proteins via aptamer domains...using RNA scaffolds to assemble proteins in vivo. Nature protocols. 7(10) 1797-1807 (2012). PubMed. ...

  17. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ... Julie from Novartis who was working with cell culture and who was extremely helpful since she was familiar...useful pieces of information from the workshop. Future workshops for Addgene Addgene strives to be a great...

  18. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...? Check Out Our Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from...incorporation of a 3’-5’ exonuclease in the reaction mixture. The exonuclease removes mismatched 3’ overhangs...

  19. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...with each other. Below we will outline the common features as well as the differences within their viral ...Kulanayake, S., Tikoo, S. K. Adenovirus Core Proteins: Structure and Function. Viruses. 2021 13(3):388. DOI:10.3390...

  20. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...variable being manipulated. Thus, the adjustable nature of viral libraries makes them the ideal vector ...low immunogenicity, and cell-specific targeting features make them an ideal delivery system for human patients...

  22. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...lentiCas9-blast to generate Cas9-expressing cells. To ensure that this growth rate effect doesn’t confound your... that express cre recombinase. Typically, this feature is used to attain cell-specific expression in transgenic...

  23. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...modified Tango beta-arrestin recruitment assay to measure receptor activation for more than 300 GPCRs. In...peroxidase-catalyzed proximity labeling to quantifiably measure GPCR agonist response in vivo (Paek et al., 2017...

  24. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...synapsin-1, human synapsin, hSynap. Matching We capture multiple variations of the same information in ...resources from the Addgene blog Learn about other features on Addgene's website Find a step-by-step guide...

  28. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...integrated (once or many times) into the genome. Make sure to choose an appropriate promoter to express your...immediate and target-specific protein degradation. Nature Chemical Biology. May 2018. 14(5): 431-441. PubMed...

  30. Rabies and Neuronal Tracing

    Type
    Blog Post
    ... of us learned in biology is that the body’s architecture and plumbing are important. We started with ...oxygenates the body. However, in neuroscience, this architecture is frequently still an open question. The connections...

  31. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed a fusion of keratin...GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth Lab now...

  33. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ..., in the form of workshops, presentations, and lectures. There are currently around 1,000 active makerspaces...discarded. For instance, the centrifuge in the picture had a broken controller and would not spin. It ...

  34. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...triplicate.  The nice thing about qPCR is that since it measures relative quantification - that is, since you’re...comparison of assays for accurate copy number measurement of the low-affinity Fc gamma receptor genes FCGR3A...

  35. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...here, the SMASh tag does offer a few other unique features. If your protein is not amenable to being tagged...effective it will be, given the diversity of protein structures, interacting partners, and localization. Thankfully...

  36. PhD Applications After COVID

    Type
    Blog Post
    ...presentation. It can help soothe your nerves and make sure you cover everything you need to during the interview...students; an online meeting can take some of that pressure off. This question and answer process can also...

  39. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...much more predictable than NHEJ. As seen in the figure above, short (5-25 bp) regions of homology flanking...knocking the GFP-Puro cassette into the locus (see figure below). The double-MMEJ strategy looks very similar...

  40. How to Be an Excellent Trainee

    Type
    Blog Post
    ... a little assistance at some point during the procedure. Be an active partner Everyone’s learning style...printed protocol, or maybe you would like to take pictures/videos of a machine set up while your mentor is...

  43. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...process that will avoid such miscommunications in the future. Assumptions: Making incorrect assumptions can...Resolving conflicts is great, but avoiding unnecessary future conflicts while giving people the tools to resolve...

  45. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...101 covers everything from plasmids parts and nomenclature to popular cloning techniques and troubleshooting...

  50. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...photos and slideshows shared some of the moments we captured over the years, including an album of Addgenie...
Showing: 851 - 900 of 1025 results