We narrowed to 1,042 results for: URE

...started making regulations such as preventing manufacturers from dumping hazardous waste directly into the...levels. Disposables are an accepted part of lab culture, but with the right incentives (for example, by...

...King soundtrack and writing this post, it is my pleasure to announce that we once again reached new heights... remember the hazards of that chemical in your obscure growth medium, these apps can help make your lab...

...pooled format at a more-typical scale of tissue culture while still offering the potential for true biological...and minimize off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed...

...Rather, they relied on published studies in the literature to curate the perfect library, obtained the appropriate...It could [be used to screen for] morphological features, migration, or survival in different contexts....

...agencies with over $100 million in annual R&D expenditures to support increased public access to the results... guided by aspirations to promote open science, ensure good stewardship of clinical trial data, serve ...

...based on how each one impacted cell viability (see figure). Most of the mutations were found to be “neutral... gene aberration drivers of pancreatic cancer." Nature communications 7 (2016): 10500. PubMed PMID: 26806015...

... can’t find the molecular cloning guide you were sure was in one of those four white binders. Thirty minutes...) and place them on an easy to reach shelf. Make sure all notebooks are visibly labeled on the spine. ...

...Plasticity in Mentoring" A recent post on the blog “Tenure, She Wrote” is titled, “What Kind of Mentor Do ...answers to the other six mentoring questions in future posts. If you have a question about mentoring that...

...complemented each other's strengths: Ginkgo Bioworks’ infrastructure allowed them to synthesize new plasmids quickly...enables science to determine the three dimensional structures of proteins.  The RESOLUTE Consortium collection...

...need to be able to replicate in Agrobacterium to ensure that they are maintained during co-cultivation ...plasmid can also be customized due to its modular structure. Two multiple cloning sites (MCS's) flank the ...

...targeted with optical stimuli. The restricted light exposure to a specific area in combination with delivery...information from a region. In the example shown in Figure 1, injected retrograde AAVs at one location result...

...answer to that question. Additionally, a concurrent Nature Biotechnology article describes the development...Tan E-P, Del Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology. 2013 Dec 23. (Article). Read more...

... a closer look at the rep and cap genes. Figure 1. The RepCap plasmid and its products. Created...265 . https://doi.org/10.1016/j.omtm.2019.01.008  Maurer AC, Weitzman MD (2020) Adeno-Associated Virus Genome...

...depends on what you are looking to study. Figure 1: A schematic showing the differences in chemogenetics...Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-010814-124803 Vlasov, K., Van Dort, C....

... introduce genes into an organism’s genome (see figure) and to disrupt endogenous genes at the site of...antibiotic-resistance genes conducted in HeLa cells). In their Nature Genetics paper, the lab showed that efficiencies...

...mechanisms for feedback (more on this below). Make sure to give positive feedback when it is merited. Surprisingly...unnecessarily big issue for you and for the employee. Make sure to focus on the action, not the person - intent ...

...some of our data at this meeting. We weren't quite sure what to expect in terms of interest, but visitors... to people who were curious about these newest features of our website.  I want to offer a huge thanks...

...-life balance. It is harder to manage when the measure of work is not just time spent at the desk, but...place as described by Sebastiano Massaro in his Nature Biotechnology article “Managing knowledge-intensive...

...Publication policy with two changes that will help us ensure that plasmids don’t get left behind in this status...Addgene! We'll continue to review our policies to make sure they meet our goals, maintain your trust, and meet...

...with the small solubility tag GB1. In SunTag nomenclature throughout the rest of this blog post, GFP refers...the GCN4 sequence to increase its alpha-helical structure and stability, creating v4 SunTag. Since the v4...

... far-eastern islands,” said Tetsushi Sakuma, a lecturer at Hiroshima University who works with Takashi...deposit. Begin yours here. It has been my genuine pleasure to send these small tokens of thanks for the incredible...

...new. Science involves success and, more often, failure. It’s useful for non-scientists to understand not...science and society 6)   Consider participating in a future SciFund Challenge. While the goal is ostensibly...

... been difficult to modify in the past.   Figure 1: Identification and enrichment use of XMAS-TREE...splice acceptor and donor sites, and introducing premature stop codons.    New from the viral service By...

... tool. They can search the database by certain features like serotype, promoter, or tool type and each...use these questions as a guide for designing your future injection experiments. Have your data or lab notebook...

... "Viral Expression Vectors" chapters, and more pictures and pro tips.  Happy reading! Learn more about...PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids: A Proposal.”Bacteriological...

...interspaced with linkers that can fold into a secondary structure that can bind specific proteins via aptamer domains...using RNA scaffolds to assemble proteins in vivo. Nature protocols. 7(10) 1797-1807 (2012). PubMed. ...

...edit, and I want to make sure that I (and Addgene) don’t forget this in the future. Here’s a list of blog...

...through biotin-avidin binding could provide a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand...map and cartoon below). Including biotin in your culture and inducing protein expression with IPTG results...

...questions help us expand and improve our resources for future users. We’re excited to be distributing new types...fulfilled more than 140 orders for virus! We’ll be sure to keep you updated on our progress and would love...

...lanceolatum in 2012. Using structural modeling and structure-guided direct evolution, Nathan Shaner and colleagues...protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407. PubMed PMID: 23524392...

...applications in cellular biology. Van Haren J et al. Nature Cell Biology 2018 Pubmed PMID 29379139. The “...Riboglow platform at Addgene. Braselmann E, et al. Nature Chemical Biology. 2018 PubMed PMID: 30061719. ...

...spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine...Looger’s lab at Janelia for neuroscientists to measure glutamate levels. iGluSnFR.A184S, a variant with...

...impact and, in turn, the big picture of the project. For example, how can future regulations impact your work...

... Julie from Novartis who was working with cell culture and who was extremely helpful since she was familiar...useful pieces of information from the workshop. Future workshops for Addgene Addgene strives to be a great...

...? Check Out Our Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from...incorporation of a 3’-5’ exonuclease in the reaction mixture. The exonuclease removes mismatched 3’ overhangs...

...with each other. Below we will outline the common features as well as the differences within their viral ...Kulanayake, S., Tikoo, S. K. Adenovirus Core Proteins: Structure and Function. Viruses. 2021 13(3):388. DOI:10.3390...

...variable being manipulated. Thus, the adjustable nature of viral libraries makes them the ideal vector ...low immunogenicity, and cell-specific targeting features make them an ideal delivery system for human patients...

... 3. How would you like to select for your flies? Sure, the vector will have an antibiotic resistance gene...differentiation by antagonizing RORγt activity. Nature. 472, 486–90 (2011). PubMed PMID: 21441909. PubMed...

...lentiCas9-blast to generate Cas9-expressing cells. To ensure that this growth rate effect doesn’t confound your... that express cre recombinase. Typically, this feature is used to attain cell-specific expression in transgenic...

...modified Tango beta-arrestin recruitment assay to measure receptor activation for more than 300 GPCRs. In...peroxidase-catalyzed proximity labeling to quantifiably measure GPCR agonist response in vivo (Paek et al., 2017...

...synapsin-1, human synapsin, hSynap. Matching We capture multiple variations of the same information in ...resources from the Addgene blog Learn about other features on Addgene's website Find a step-by-step guide...

...information so you can tap them for advice. As a future alum, these are people you can “cold call” and ...time. Consider this option but be cautious and make sure to choose one that comes highly recommended from...

...the plan of being a scientist. I pretty quickly figured out that I just don’t have the patience to be a...Career Channel of the blog so you don’t miss out on future career posts! Additional Resources on the Addgene...

...integrated (once or many times) into the genome. Make sure to choose an appropriate promoter to express your...immediate and target-specific protein degradation. Nature Chemical Biology. May 2018. 14(5): 431-441. PubMed...

...phenotypes. Factors such as the growth medium, growth temperature, and growth rate can all impact plasmid copy ...gene expression at any copy number in bacteria." Nature biotechnology 36.4 (2018): 352. PubMed PMID: 29553576...

...reporter output was observed in their 293 cell culture system. In cells cotransfected with GFP and the...types of proteins. For proteins with a modular structure, constructing split variants should be relatively...

...spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed a fusion of keratin...GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth Lab now...

...Being a leader in an organization, you need to make sure the organization speaks in “one voice” whilst among...only assist you and make you more qualified for future career opportunities. Becoming involved is not ...

..., in the form of workshops, presentations, and lectures. There are currently around 1,000 active makerspaces...discarded. For instance, the centrifuge in the picture had a broken controller and would not spin. It ...

... of us learned in biology is that the body’s architecture and plumbing are important. We started with ...oxygenates the body. However, in neuroscience, this architecture is frequently still an open question. The connections...