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We narrowed to 1,092 results for: addgene

Showing: 851 - 900 of 1092 results

  1. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...Blotting Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...10.1038/nmeth.3995 Additional Resources on the Addgene blog Learn about research applications of antibodies...

  2. An Introduction to Adenovirus

    Type
    Blog Post
    ...pre-existing toolbox for adenoviral vectors in Addgene’s repository is smaller than for other vector types...1566523213666131125095046. PMID: 24279313. More resources on the Addgene blog Viral Vectors 101: Viral Applications Adenoviral...new adenoviral vectors. This post was written by Addgenie Brian O'Neill.  References and resources References...

  3. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...Career Options Resources on Addgene.org Learn more About Careers at Addgene ...gardener, and cook.  Additional Resources on the Addgene Blog Academic v. Non-Academic Career Decisions...

  4. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...  Find broad host range recombinant vectors at Addgene!   References and resources References Doran, ...10.1007/s13205-023-03507-0  More resources on the Addgene blog Plasmids 101: Origin of Replication Plasmids...

  5. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...Resources on Addgene.org: Check out the lentivirus guide Find ready-to-use lentiviral preps at Addgene ...j.neuron.2007.01.033 Additional resources on the Addgene blog: Read the Quick Guide to All Things Lentivirus...

  6. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ... Resources on Addgene.org Download the Plasmids 101 eBook  Browse plasmids at Addgene Read our Molecular...Kamruzzaman et al., 2017). Additional resources on the Addgene blog Read all of our Plasmids 101 blog posts Learn...

  8. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... then see how many of them have requested from Addgene. It is always gratifying to see all the hands go...Herminia Ibarra and Kent Lineback Resources on the Addgene Blog Check Out Our Networking for Scientists Blog...

  9. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...    Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory at Columbia...doi.org/10.1038/nbt.3149 Additional Resources on the Addgene Blog Learn how to Choose the Best Cas9 Variant... CRISPR Has Been Used with AAV Resources on Addgene.org Find Cas9 Activators Find Cas9 Repressors Catch...

  10. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...when following protocols. Need Virus? Check out Addgene's New Viral Service! References 1. Hay, R.J., Macy... PMCID: PMC514534. Additional Resources on the Addgene Blog Get Tips for Titering Your Lentivirus Learn... Lentiviral Vectors Additional Resources on Addgene.org Find Viral Vectors for Your Research Brush Up...

  11. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...cytosine within a particular sequence. Browse Addgene's curated list of Bacterial Expression Systems Table...tips and tricks! Leave a comment below or browse Addgene’s collection of empty backbones.   Our Most Popular... 101 Articles?  Find them all here: http://blog.addgene.org/topic/plasmids-101   ...

  13. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...available to researchers - some readily found at Addgene. Shining light on transcriptional activation ...Doug Mortlock on A CRISPR Blog Resources on the Addgene Blog Read Other Optogenetics Blog Posts Read Our...Our Interview with Ed Boyden Resources on the Addgene Website Browse Our Collection of Optogenetics ... Various Addgenies...

  19. Let There Be LITE Plasmids

    Type
    Blog Post
    ...’s team devised the plasmids now available in Addgene’s repository for use in neurons, both in culture...

  20. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...good option for your experiment? Look it up in Addgene’s Data Hub and the CLOVER repository of published...Table 1: Systemic capsid plasmids available from Addgene Capsid Published Tropism Model Organism...most commonly requested systemic capsid in the Addgene repository. Like its parent AAV-PHP-B, it can be...repository of published systemic AAV experiments and Addgene’s AAV Data Hub to learn more about the capsid you...significant contributions from Cynthia Arokiaraj. Addgene would also like to thank Viviana Gradinaru and ...10.1038/s41596-018-0097-3 More resources on the Addgene blog Viral Vectors 101: AAV Variables That Matter...

  22. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...deposited their editors, called gTBE and gCBE, with Addgene. gTBE can introduce T → C and T → G edits, though...s41467-024-50012-w Additional Resources on the Addgene Blog CRISPR 101: Cytosine and Adenine Base Editors...about four base editor reporters Resources on Addgene.org Browse CRISPR plasmids for base editing Get ...

  23. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...of temperatures varying between 10-50°C. Visit Addgene's COVID-19 Plasmids and Resources Many thanks ...20detection%20(updated).pdf Additional resources on the Addgene blog Read more about SHERLOCK and DETECTR Learn...available for COVID-19 research Resources on Addgene.org Get the basics about CRISPR in our CRISPR guide...

  24. March for Science

    Type
    Blog Post
    ...author do not represent a formal stance taken by Addgene or its staff. In Washington, D.C. as well as sister...author do not represent a formal stance taken by Addgene or its staff. Many thanks to our guest blogger,...

  25. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ... a common source of conflict. For instance, at Addgene, there are two teams whose primary role is customer... Jan. 3, 2022.     Additional Resources on the Addgene blog Management for ScientistsTransferable Skills...

  26. A Control for All Seasons

    Type
    Blog Post
    ...Selecting the Right Antibody Resources on Addgene.org Addgene's Antibody Guide  ...Central PMCID: PMC2712964. More resources on the Addgene blog Antibodies 101: Validation Antibodies 101:...

  27. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...little as an hour. Find the SHERLOCK plasmids at Addgene! Applications of the SHERLOCK detection system ...j.cell.2015.09.038 Additional resources on the Addgene Blog Learn about using toehold switches to detect...multiplex genome editing abilities Resources on Addgene.org Checkout our CRISPR guide page Browse the CRISPR...

  28. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...biology Resources on Addgene.org Find all CRISPR plasmids and resources at Addgene Find CRISPR base editors...Laboratory (2018). Additional resources on the Addgene blog Read all of our CRISPR 101 blog posts Download...

  29. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... plasmids that contain both AviTag and BirA at Addgene! Oligonucleotide labeling Biotin can be added to...PubMed PMID: 8245422. Additional resources on the Addgene blog In vivo biotinylation of bacterial fusion...

  30. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...the J. Craig Venter Institute in 2009. Here at Addgene we added this option to our drop down menu of common...Assembly Overview Additional Resources on the Addgene Blog Browse All of Our Plasmids 101 Posts Read... Cloning Techniques Additional Resources on Addgene.org Find Plasmid Cloning Protocols Find Virus Protocols...

  31. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...DNA and lentiviral/retroviral vectors. Used by Addgene for pooled library amplification. F' mcrA Δ(mcrBC-hsdRMS-mrr...of genetic markers for your reference. Browse Addgene's curated list of Bacterial Expression Systems. ...

  32. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...! To find more viral vectors available through Addgene, be sure to check out our viral service page and...Viral Vector Blog Posts Additional Resources on Addgene.org Check out our Viral Service Page Browse Additional...

  35. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology Pages The problem: Host binding proteins...PubMed Central PMCID: PMC2258639. Resources on the Addgene Blog Learn How CRISPR Can Be Combined with Phage...CRISPR For Genome-Wide Screens Resources on Addgene.org Visit Our Microbiology Pages Use the FX Cloning...

  36. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ... As you know well if you’ve been following the Addgene blog, it has been repurposed for genome editing...https://doi.org/10.1038/srep32565 Resources on the Addgene Blog CRISPR 101: Mammalian Expression Systems ...Depression Read About Brainbow Resources on Addgene.org Find CRISPR Plasmids for use in Mammals Check...

  37. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...explore genome editing approaches for exon skipping. Addgene depositor Charles Gersbach used paired zinc finger...PMCID: PMC4393360. Additional Resources on the Addgene Blog Learn How CRISPR Could Be Used to Treat Cataracts...Used to Delete Genes Additional Resources on Addgene.org Check out Our CRISPR Guide Pages Find CRISPR...

  39. Science as Art

    Type
    Blog Post
    ...on the Addgene Blog Learn About Experimenting in an Open Source Lab Resources on Addgene.org Try the...

  40. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...10.1089/hum.2020.069  Additional resources on the Addgene blog: Discover how the PHP.eB capsid was engineered...parts of the AAV transfer plasmid  Resources on Addgene.org Learn about AAV Find AAVs for your experiment...

  41. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...1874070701307010010 Additional resources on the Addgene blog Find five ways to purify DNA without a kit...Browse our Plasmids 101 blog posts Resources on Addgene.org Check out the Molecular Biology Reference Find...

  49. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...polymerase by Outreach Scientist, Andy Baltus. Addgene founder, Melina Fan, recently shared our protocol...recycling programs and it seems to me that most Addgenies make an effort to avoid plastic bags and to conserve...Actually, I was happy to hear that I am not the only Addgenie who has a thrifty streak.  One of the most accessed...
Showing: 851 - 900 of 1092 results