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1. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   Blog Post

   ...tropism of Nanoblades, different viral envelope protein(s) are expressed to pseudotype Nanoblades. The team ...

2. 3D Printing Meets CRISPR Cas9

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   Blog Post

   ...interactive images of their proteins. Since the 1980’s, journal articles have been filled with impressive...

3. Fluorescent Proteins 101: Introduction to FRET

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   Blog Post

   ...in drug discovery. References 1. Baird, Geoffrey S., et al. "Circular permutation and receptor insertion...

4. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   Blog Post

   ...https://doi.org/10.1016/j.neuron.2013.08.032.  Morita S, Noguchi H, Horii T et al. (2016) Targeted DNA demethylation...

5. A Look at Addgene's QC Process

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   Blog Post

   ... the de novo NGS result to the deposited sequence(s) in SnapGene. SnapGene annotates the plasmid maps ...

6. Progress Towards a PAM-Free CRISPR

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   Blog Post

   ... Resources References Kwart, D., Paquet, D., Teo, S. et al. Precise and efficient scarless genome editing...

7. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   Blog Post

   ...overlapping markers. Compensation/reference control(s) Compensation or “reference” controls are samples ...

8. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   ...//doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD...

9. Tips for Technical Support Calls

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   Blog Post

   ... also let us know! Sometimes those few “thank you”s are what get a customer service agent through a busy...

10. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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    ...Pubmed Central PMCID: PMC4866822. 3. McDowell, Gary S., et al. "Shaping the Future of Research: a perspective...

11. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    Blog Post

    ...science.aaa5945. PubMed. DiCarlo, J. E., Chavez, A., Dietz, S. L., Esvelt, K. M. & Church, G. M. RNA-guided gene...

12. 15 Hot Plasmids from 2017

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    Blog Post

    ...their useful reagents shared via Addgene. Narayan S, et al. Cell Reports. 2017. PubMed PMID: 28813671 ...the popular type II CRISPR systems, which include S. pyogenes (Spy) Cas9, been identified. In Rauch, et...

13. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    Blog Post

    ...in several bacteria associated with human disease (S. aureus, Klebsiella pneumoniae, and P. aeruginosa)...

14. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    ...PubMed Central PMCID: PMC3074624. 5. Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent...

15. Summer SciComm Series: Modes of Communication

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    Blog Post

    ...following four questions to figure out which method(s) work best for you, your audience, and your science...

16. Pooled Library Amplifications

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    Blog Post

    ...and how many cells to use for your transformation(s). The amount of competent cells you need total and...

17. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post

    ...References and resources References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding...

18. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post

    ...DNA-scissors (blog). Blog website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications...

19. Your Lentiviral Plasmid FAQs Answered

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    Blog Post

    ...Many transfer vectors are SIN. Packaging plasmid(s) contain the minimal number of HIV genes required ...

20. Plasmids 101: Codon usage bias

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    Blog Post

    ...well correlated with codon bias in both E. coli and S. cerevisiae. Protein folding - If a protein is encoded...