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We narrowed to 712 results for: BASE

Showing: 341 - 360 of 712 results

  1. The time and cost required to make a plasmid

    Type
    Blog Post
    ...hours of hands-on time to make a single plasmid, based on the assumptions outlined in Table 1.   Of course...performing the experiments. This cost varies hugely based on a number of factors, but we can assume it’s at...

  2. Summer SciComm: Preprints

    Type
    Blog Post
    ...critiques or endorsements. In contrast, journal-based peer review is usually a confidential process among...ensure a manuscript’s quality. But even journal-based peer review can be prone to biases or errors, with...

  3. Antibodies 101: Isotypes

    Type
    Blog Post
    ...staining. Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...of two. There are four IgG subclasses, numbered based on their abundance in blood: IgG1, IgG2, IgG3, and...

  6. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...by gel or by nanodrop to assemble the  fragments based on their molar ratio. Assembly of the fragments ...you can assemble the different parts of a plasmid based on the cloning strategy you followed. While most...

  7. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...the PCR is complete, the product can be verified based on size by gel electrophoresis . Protocol Video ...hydrogen bonds are broken between the nucleotide base pairs. The initial denaturation step is commonly...temperature (Tm). Set extension step at 1-2 minutes per kilobase of product depending on whether you are using ...

  8. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    ...Open Hardware? Author: Benedict Diederich Course-Based Undergraduate Research Experiences (CUREs)Author...AccessibleResearcher: Ryan Mehl; Andy KarplusIBEX Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers...YanevCellosaurus: A Cell Line Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic ...

  9. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...that covers most things CRISPR - from nickases to base editing to pooled library screening. It also has...

  10. Microbiology Resources

    Type
    Collection
    ...supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression - Pertz Lab Bacterial...Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window) Return to top...

  11. Worm Expression Resources

    Type
    Collection
    ...with CRISPR/Cas9 in Caenorhabditis elegans. Mos1 Based Transgenesis miniMos Transgenesis Kit - Erik Jorgensen...WormAtlas - A database featuring behavioral and structural anatomoy of C. elegans. WormBase - A consortium...

  12. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...causes of irreproducibility with some solutions based on what I’ve learned. I hope you can apply some ...all human extracellular proteins with community-based validation. They also have plans to expand to other...with larger and larger datasets and use computer based analysis techniques, an important component of reproducibility...on the Addgene Blog FPbase: A new community-editable fluorescent protein database ReFigure: Save Scientific...technologies later. Organizations such as BenchSci and FPbase are working hard to enable researchers by making...BenchSci collates information about antibodies while FPbase curates information about fluorescent proteins ...

  13. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...TAG. Design your repair template with 55-100 base homology arms upstream and downstream of the insertion...using the ivTRT method, try to make the first two bases of the left homology arm guanine (i.e. GG); this... PMC3961003  Miura, Hiromi, et al. “CRISPR/Cas9-based generation of knockdown mice by intronic insertion...

  14. The 12 Days of CRISPR

    Type
    Blog Post
    ...the 3rd day of CRISPR, Addgene gave to me: dCas9-based gene editing for cleavage-free genomic knock-in ...

  16. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...the concentration of proteins in an ELISA well is based on a colorimetric reaction. Most commonly, this ...concentration of protein in the experimental wells based on the equation of the line. With these tips in ...

  17. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...track cell-cell interactions By Jennifer Tsang GFP-based Touching Nexus (G-baToN) is a new tool developed... quantification of this depletion using imaging-based measurements (Ashley et al., 2021).   Schematic...

  18. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...the Rare Genomics Institute by providing plasmid-based tools for rare disease research. Congratulations...events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors to monitor intracellular signaling...

  19. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...protein for live cell imaging would be mTurquoise2, based on the data acquired at low excitation powers. Note...Amsterdam) where she develops genetically encoded FRET based biosensors.     Additional Resources on the Addgene...

  20. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ... on the identification of autophagic structures based on morphology. Autophagosomes are relatively easy...summary of what color fluorescence Rosella emits based on its localization. Table 1: Summary of Rosella...
Showing: 341 - 360 of 712 results