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We narrowed to 644 results for: CAR

Showing: 341 - 360 of 644 results

  1. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...institute where their typical workdays will be carried out until the end of the appointment. The visiting...don’t forget to have fun! Visiting is an amazing career opportunity, but also a really good time! If it...

  2. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...Number of chromosomes 16 3 4 Preferred carbon source Glucose Glucose Glucose, methanol,...meaning it can utilize methanol as its sole source of carbon and energy (as well as glucose or glycerol). K....

  3. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...cell on their way to the nucleus where their gene cargoes can be expressed. The bottom line is that a lot...particles do not remain adhered to the membrane. Be careful when diluting - if you end up concentrating your...

  4. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...decolorized as gram negative. A German scientist, Carl Weigert, later expanded on Gram’s discovery, adding...resources.   Bruckner, M. Gram Staining. https://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html...

  6. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...compromising on the relevant science."     - Randall Carter, Contracts Administrator Inferior: How Science ...Story by Angela Saini "This book is a smart and carefully presented case for better more diverse science...

  7. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...larger. And since the library contains many plasmids, care must be taken to ensure that all of these plasmids...powerful research tool, but their proper use requires careful amplification and maintenance of good representation...

  8. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...encapsulated proteins. Video by ACS. For a more cartoony explanation of R-bodies, check out this video....Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth. Biol. (2016). doi:10.1021/acssynbio.5b00237...

  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...intensity and/or duration. Both of these strategies carry the risk of off-target effects. Harald Janovjak ...of both DsRed fluorescence and GFP shRNA. Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre alleles...lack release factor one (RF-1; Bacterial strain EcAR7) and minimizing the set of plasmids required to ...

  10. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...regions of the brain. However, the Cre-dependent AAV cargo would only become activated in the presence of Cre...design and interpretation of results. Take extra care when evaluating how this serotype works in your ...

  11. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ... be assembled in a unique order with no cloning scars. The Elledge Lab showed that a five-piece assembly...researchers doing large amounts of cloning. Assembly is scarless, unlike Gateway cloning, and the method’s flexibility...

  12. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...well, so make sure you’re taking time to do them carefully and thoroughly. Have a physical copy on hand as... to Getting Started in Undergrad Research Early Career Researcher Toolbox  8 Tactics for Choosing a Lab...

  13. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...clearly and strategically is powerful in almost any career. It allows you to share and build support for your...with two single-panel figures, one of which was a cartoon of the protein.) In the middle of my presentation...

  15. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...PEG10 RNA sequences to instead drive packaging of cargo sequences in the capsid, in an approach called Selective...Figure 1: SEND combines an endogenous Gag homolog, cargo RNA, and fusogen in a modular system for delivering...

  20. Working with Nuclear Receptors

    Type
    Blog Post
    ..., including hypertension, cancer, diabetes, cardiovascular disease, cholesterol gallstone disease, senescent...such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. This attendant signaling feature...
Showing: 341 - 360 of 644 results