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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 641 results for: CAR

Showing: 341 - 360 of 641 results

  1. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...larger. And since the library contains many plasmids, care must be taken to ensure that all of these plasmids...powerful research tool, but their proper use requires careful amplification and maintenance of good representation...

  3. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...institute where their typical workdays will be carried out until the end of the appointment. The visiting...don’t forget to have fun! Visiting is an amazing career opportunity, but also a really good time! If it...

  4. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...Number of chromosomes 16 3 4 Preferred carbon source Glucose Glucose Glucose, methanol,...meaning it can utilize methanol as its sole source of carbon and energy (as well as glucose or glycerol). K....

  5. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...encapsulated proteins. Video by ACS. For a more cartoony explanation of R-bodies, check out this video....Piston That Breaks Membranes to Release Encapsulated Cargo. ACS Synth. Biol. (2016). doi:10.1021/acssynbio.5b00237...

  6. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...intensity and/or duration. Both of these strategies carry the risk of off-target effects. Harald Janovjak ...of both DsRed fluorescence and GFP shRNA. Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre alleles...lack release factor one (RF-1; Bacterial strain EcAR7) and minimizing the set of plasmids required to ...

  7. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...well, so make sure you’re taking time to do them carefully and thoroughly. Have a physical copy on hand as... to Getting Started in Undergrad Research Early Career Researcher Toolbox  8 Tactics for Choosing a Lab...

  8. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ... be assembled in a unique order with no cloning scars. The Elledge Lab showed that a five-piece assembly...researchers doing large amounts of cloning. Assembly is scarless, unlike Gateway cloning, and the method’s flexibility...

  9. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...regions of the brain. However, the Cre-dependent AAV cargo would only become activated in the presence of Cre...design and interpretation of results. Take extra care when evaluating how this serotype works in your ...

  10. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...PEG10 RNA sequences to instead drive packaging of cargo sequences in the capsid, in an approach called Selective...Figure 1: SEND combines an endogenous Gag homolog, cargo RNA, and fusogen in a modular system for delivering...

  15. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...clearly and strategically is powerful in almost any career. It allows you to share and build support for your...with two single-panel figures, one of which was a cartoon of the protein.) In the middle of my presentation...

  17. Working with Nuclear Receptors

    Type
    Blog Post
    ..., including hypertension, cancer, diabetes, cardiovascular disease, cholesterol gallstone disease, senescent...such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. This attendant signaling feature...

  18. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...not yet fully understood, so it is important to carefully select the target locations of knock-in studies...systems offer a number of different options. By carefully considering your ncRNA of interest, you should...

  19. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...for follow-up DNA oligos to arrive. We also were careful to set up clear tasks for each member of the team...Sciences at Arizona State University. His group carries out research in synthetic biology, nanomaterials...

  20. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...the molecular biology to learning how to grow and care for the worms. As an example of one of the problems...cheap using equipment that would otherwise be discarded. For instance, the centrifuge in the picture had...
Showing: 341 - 360 of 641 results