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We narrowed to 649 results for: CHI

Showing: 341 - 360 of 649 results

  1. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...cells. Positive selection in mammalian cells To achieve stable transfection, there should be a selective...required for efficient selection. This is usually achieved by performing a "kill curve" (basically growing...

  2. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...non-interacting with) the cells’ own natural translation machinery; these are often incorporated into the cells via...of Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https://doi.org/10.21769/BioProtoc...

  3. Hot Plasmids - February 2022

    Type
    Blog Post
    ...tool for labeling. Here, Liu et al. engineer a chimeric single chain variable fragment (scFv), grafting... which also provides a useful yet low-pressure teaching opportunity for lab members or students new to...

  4. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ... which is then read by a detector panel in the machine. Fluorophores are light sensitive, so antibodies...idea to make sure that laser is available in your machine before you make your purchase. Picking a single...

  6. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...approaches. However, with each new technical achievement, the complexity of the device increases, as does...with the community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation...

  7. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...recombineering, bacteria express phage recombination machinery, which facilitates easy genetic modification by...electrocompetent cells in which the phage recombination machinery is induced using L-arabinose. These cells can ...

  9. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ... of its biggest advantages. Specificity can be achieved using specialized promoters, Flp or Cre dependent...expression AAVs have to infect cells, hijack host cell machinery, and synthesize a second strand of DNA before ...

  10. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ... a moment and honor all of the success we have achieved and the opportunities we have for the future. ...successful candidates from internal referrals. Our long reaching reputation helps us hire great people from farther...

  11. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...approximately the length of your average PhD to achieve!) Each field has its own framework: a particular...images, and links to pay attention to. If you’re watching a video, you now have moving images, noises and...

  12. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...When you’re searching for an antibody to use in your next experiment, you’ll probably notice a lot of...regions of the protein may be masked by crosslinking (ChIP) or fixation (IHC). Because of their increased sensitivity...

  13. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...(MAGIC). With MAGIC, scientists introduce an Escherichia coli donor strain into the microbiome to deliver...segregates into one cell during cell division. To achieve the agglomeration of fluorescent proteins into ...

  15. Antibodies 101: Conjugation

    Type
    Blog Post
    ...to your antibody. This chemical coupling can be achieved through several different mechanisms. Most take...10.1016/j.it.2012.02.010 Dennler P, Fischer E, Schibli R (2015) Antibody Conjugates: From Heterogeneous...

  16. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...DNA-protein interactions or to analyze chromatin states (CHIP-seq, FAIRE-seq, Cut & Run) and to detect protein-protein...pulldown of a protein of interest, much like using ChIP for studying protein and DNA interactions. Unfortunately...

  19. Working with Nuclear Receptors

    Type
    Blog Post
    ...Biology at Baylor College of Medicine and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear...of Molecular and Cellular Biology, and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear...

  20. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...While some of the statements may be slightly far reaching, remember that you are writing a proposal to test...secondary milestones to meet along the way to achieving each aim. A funded proposal typically lists the...
Showing: 341 - 360 of 649 results