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We narrowed to 824 results for: EED

Showing: 341 - 360 of 824 results

  1. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...regulations impact your work? What design variables need consideration? Enrich your knowledge and skills ...started: Planning your career path and identifying needed skills First, evaluate your skills, values, and...and learn new skills, while also getting a much-needed break from your lab bench.  Want further information...

  2. The Power Behind NGS Plasmid Validation: seqWell

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    Blog Post
    ...Sanger sequencing have historically matched the needs of most molecular biology labs. Recent and rapid...samples are brought in line with each other before proceeding to library prep. Even with this “pre-prep” normalization...libraries prepared from multiple samples will then also need to be quantified and re-normalized in order to be...

  3. The MTA at Addgene

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    Blog Post
    ...system for this process. We often get positive feedback on “our” MTAs, which sometimes makes people think... out your plasmid hasn’t shipped because a form needs to be signed. But the truth is, meaningful progress...offices with the Golden Stopwatch award and seek feedback as to how we can make our process easier for others...

  4. 25 Work From Home Tips for Scientists

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    Blog Post
    ...as possible so that you can adapt to your kids' needs. Plan ahead. Think about and set up activities that...Write a manuscript. If you have all the data you need to begin writing a full paper, that’s great. Otherwise...done without having access to your lab or even needing to have collected data. Work on your dissertation...

  5. What Good Citizenship Can Do for Reproducibility in Science

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    Blog Post
    ...the methods section of a paper is the one that you need to spend the least time on—just put down a basic... What if that could change? Everyone in science needs both a job and funding, so institutions and funders...best approach to doing that will be, and it will need some effort and experimentation. But it ought to...

  7. FPbase: A new community-editable fluorescent protein database

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    Blog Post
    ...Research Associate at Harvard Medical School. The need for a community fluorescent protein database As ...etc...) and a more robust database structure was needed.  It is also difficult to scale such a resource...photobleaching comparisons.  We very much appreciate feedback, so try out the site and contact us if you have...

  8. 5 Great Apps for Lab Life

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    Blog Post
    ... allots for 25 MB of space per user, but, if you need more space, you can sign up for a plan. Best of ...use and that indicates if a sequential digest is needed. The enzyme finder is also a very helpful aspect... adding a restriction site to a primer, you only need to skim the alphabet on the side of the screen to...

  9. Addgene Welcomes John Doench to the Advisory Board

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    Blog Post
    ...and it just blew me away….  I have to come here, I need to spend time here, this is an incredible place....order to ask interesting biological questions, you need a tremendous amount of technology development alongside...throughput way now, but Doench says the technology needs to advance (become more efficient in terms of editing...

  10. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

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    Blog Post
    ...restriction sites that flank the insert. You will need to know both the approximate size of the vector ...have to use these enzymes for your digest, you will need to purify your DNA from a dcm or dam methylation-deficient...smaller bands from running off; however, you may need to run the gel for longer to achieve good separation...

  11. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    Blog Post
    ...focused ... to understand cancer and defeat it we need to attack it from all fronts!”  Here I aim to compile...tumor progression (Ref 2). Future treatments may need to target this immunosuppressive reaction in order...aspirin may give our immune system the boost it needs to help rid us of cancer in combination with chemotherapy...

  12. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ... make it easier for you to find the plasmids you need. We'll be monitoring and improving the maps further...ability to find the plasmid with the features you need quickly. SnapGene’s Feature Library - SnapGene’...and quickly identify the plasmids and features you need for your next experiment. We would like to extend...

  13. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    Blog Post
    ... the individual variants found in tumors are exceedingly rare. As a result, how most of these “variants...But, often these methods don’t agree. Confusing indeed! We've developed a new plasmid toolkit, the Broad...developing these assays, we figured that they would need to be generalizable and capable of measuring a variety...

  14. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post
    ...Across these methods, the following components are needed: γ-Retroviral transfer plasmid encoding a transgene...stably expresses Gag-Pol and/or Env, eliminating the need to deliver these genes in trans by specific plasmids...definition, at the beginning of R, is capped, and proceeds through U5 and the rest of the provirus. 3...

  15. Hot Plasmids - May 2022

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    Blog Post
    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid... recently, the approach has been hampered by the need to tag each protein of interest and, for in vivo... the approach more accessible, as it reduces the need to generate “single-use” transgenic lines. Instead...

  16. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post
    ... design phase of your experimental planning, you need more nitty-gritty details — how can I multiplex ...researchers, the previous information may be all you need to start your targeting experiment. However, many...many situations could arise where you still need more information. If you are performing a viral delivery...

  17. Viral Vectors 101: Viral Vector Elements

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    Blog Post
    ...demystify all components of viral vectors here! Need Virus? Check out Addgene's Viral Service! Types ...infiltrate and integrate into the host genome. First, it needs to be virally enveloped which is achieved by expression...matrix, capsid, and nucleocapsid viral components needed for viral packaging, assembly, and display. Pol...

  18. Viral Vectors 101: Biosensors

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    Blog Post
    ...component, which we will refer to as the analyte, needs to be specifically recognized by another factor,...viral vectors, so if you want to use them, you’ll need to use the vectors (or redesign the tools). It’s...targetability. As mentioned prior, many biosensors need to be in specific tissues or cells to monitor their...

  19. Viral Vectors 101: Types of viruses

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    Blog Post
    ...capacity of ~ 8 kb, ample room for most experimental needs, and can transduce (infect) a wide range of cell...), an alternative virus, or other strategies may need to be considered if size constraints are an issue... cargo! Adenovirus is an effective option if you need high, transient expression of a genetic cargo or...

  20. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    Blog Post
    ...recombineering strategies, only 3-4 phage proteins are needed and these can be provided via a separate plasmid...into stable circular plasmids - no nick repair is needed. Since the bacteria are subsequently grown at 37...Recombination efficiency is high enough to negate the need for selection usually required in recombineering...
Showing: 341 - 360 of 824 results