... compounds needed for growth, or auxotrophy, the inability to synthesize a compound needed to grow. Auxotrophy...on themselves. Therefore, your cloning strategy needs to identify cells containing the plasmid construct...

... allow ourselves moments of release and mental freedom. Practical approaches Engaging in mindful meditation...excel (and thus feel grateful) and identify areas in need of improvement. These unrelated ideas might hold...discoveries often emerge when we allow ourselves the freedom to explore beyond the expected. By widening your...

...conditions, and everything in between – now all you need are some of your favorite proteins purified to carry... bound to the glutathione resin, eliminating the need for an extra clean up step of the tag – a handy ...maybe try MBP if you are having solubility issues!). Need a GST antibody? Addgene has you covered! His His...

...sequence manipulation tools  Sometimes DNA sequences need to be “cleaned up” or changed in some way before...help you calculate how much insert and backbone you need for different types of cloning methods, such as ...help you choose the best CRISPR software for your needs 5 great apps for lab life  Sharing your lab protocols...

...system is composed of a promoter-less reporter which needs to integrate within an exon of a gene to generate...gene to turn on expression. Enhancer traps do not need to be integrated within an intron or exon of a gene...directly integrated into it. Enhancer traps do not need to be inside of a gene’s open reading frame, in ...

...using dog breeds as an example adults 45-55 yrs old who breed or are interested in breeding dogs who ...

... hosts you intend to use, and whether or not you need to consider your plasmid's compatibility with one...same group should not be co-transformed, so if you need two plasmids for an experiment, make sure they have...have not started to die off. Have any questions? Need something clarified? If so please, post in the comments...

... through the NIH Common Fund, emphasizes that we need more standardized methods for evaluating CRISPR ...to move the CRISPR field forward. To do that, we need to learn much more about cellular DNA repair pathways...base editors, which alter genomic DNA without the need for cellular HDR.   Having had time to digest my...

...intrepid researcher. There existed, therefore, a need for an adaptable tool that could continuously monitor...giving an idea of the stiffness the fibers would need to be, as well as other features necessary for implantation...implantation of fibers, indicating the system was indeed stable for continuous monitoring. On the gut side...

...Scientific publications are a nice to have, not a need to have Must be ready to change projects frequently...haul Limited movement in one company, likely will need to move around every 3-6 years—a short and exciting...you focus on the transferable skills that you will need to get there. Additional Resources on the Addgene...

...regulations impact your work? What design variables need consideration? Enrich your knowledge and skills ...started: Planning your career path and identifying needed skills First, evaluate your skills, values, and...and learn new skills, while also getting a much-needed break from your lab bench.  Want further information...

... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...do contain the specific ubiquitin ligase pathway needed for AID. By tagging proteins with a 7kD degron ...Cas9’s endonuclease activity can be limited by the need for a specific PAM (protospacer adjacent motif) ...

...as possible so that you can adapt to your kids' needs. Plan ahead. Think about and set up activities that...Write a manuscript. If you have all the data you need to begin writing a full paper, that’s great. Otherwise...done without having access to your lab or even needing to have collected data. Work on your dissertation...

...Research Associate at Harvard Medical School. The need for a community fluorescent protein database As ...etc...) and a more robust database structure was needed.  It is also difficult to scale such a resource...photobleaching comparisons.  We very much appreciate feedback, so try out the site and contact us if you have...

...system for this process. We often get positive feedback on “our” MTAs, which sometimes makes people think... out your plasmid hasn’t shipped because a form needs to be signed. But the truth is, meaningful progress...offices with the Golden Stopwatch award and seek feedback as to how we can make our process easier for others...

...Sanger sequencing have historically matched the needs of most molecular biology labs. Recent and rapid...samples are brought in line with each other before proceeding to library prep. Even with this “pre-prep” normalization...libraries prepared from multiple samples will then also need to be quantified and re-normalized in order to be...

... allots for 25 MB of space per user, but, if you need more space, you can sign up for a plan. Best of ...use and that indicates if a sequential digest is needed. The enzyme finder is also a very helpful aspect... adding a restriction site to a primer, you only need to skim the alphabet on the side of the screen to...

...the methods section of a paper is the one that you need to spend the least time on—just put down a basic... What if that could change? Everyone in science needs both a job and funding, so institutions and funders...best approach to doing that will be, and it will need some effort and experimentation. But it ought to...

...and it just blew me away….  I have to come here, I need to spend time here, this is an incredible place....order to ask interesting biological questions, you need a tremendous amount of technology development alongside...throughput way now, but Doench says the technology needs to advance (become more efficient in terms of editing...

...restriction sites that flank the insert. You will need to know both the approximate size of the vector ...have to use these enzymes for your digest, you will need to purify your DNA from a dcm or dam methylation-deficient...smaller bands from running off; however, you may need to run the gel for longer to achieve good separation...