We narrowed to 438 results for: ENA;
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TypeBlog Post... other unique features. If your protein is not amenable to being tagged, e.g. tagging some proteins can...
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Of Myc and Men
TypeBlog Post...1997) with the goal of making the antibody more amenable to protein engineering. As a result, you can now... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...import, and neuronal trafficking. Although FPs have enabled discovery of many cellular mechanisms, there are... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...PubMed PMID: 30675034. Peters, Jason M., et al. "Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi... -
Academic vs. Industry Postdocs
TypeBlog Post...are busy with their pipeline projects. In this scenario, finding someone with whom you can have an in-... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog Post...form complex, multi-insert modular assemblies that enable biosynthetic pathway engineering and optimization... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post...number of inserts ...as some methods are more amenable to combining multiple DNA fragments than others... -
Plasmids 101: Protein Expression
TypeBlog Post...level recombinant protein expression. These systems enable high expression of very complex, glycosylated proteins... -
Transferable Skills: Negotiation
TypeBlog Post...about the individuals, each example provides a scenario where both people get something valuable. Importantly... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post...https://doi.org/10.1038/mtna.2015.32 Wright JF, Zelenaia O (2011) Vector Characterization Methods for Quality... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post... to see what new editing possibilities MMEJ can enable. References 1. Sakuma, Tatsushi, et al. (2016)... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...information about how the vector was assembled, and enables requesting labs to utilize and modify the plasmid... -
Genome-wide Screening Using CRISPR
TypeBlog Post...compared to a non-treated control group. In this scenario, gRNAs that are “enriched” correspond to genes... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...with techniques that make tissues transparent are enabling microscopes to visualize the cellular architecture... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog Post...biology and genetics because the underlying advance enabling RNA-guided gene drives is Cas9 genome editing.... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post...interest at high levels. Unlike vectors that express screenable or selectable markers from a unique promoter,... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post...program uses a smaller word size and lower scoring penalties for mismatches and gaps in the alignment. If you... -
MXS Chaining
TypeBlog Post...directed localization of the fluorophore, and thus enabled labeling of particular structures (Table 1). Each... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...chemistry and evolution to illuminate biology and enable next-generation therapeutics. Prime editing, base... -
Droplet Digital PCR for AAV Quantitation
TypeBlog Post...Increasing the elongation time to 2 minutes and the denaturation time to 1 minute has been shown to increase ...