We narrowed to 703 results for: ESC

... articles reporting chromatin-related research describe the production of plasmids that might be useful...colleagues and guest bloggers have written posts describing new methods and tools for studying chromatin ...

...differ by only a few nucleotides, such as the fluorescent proteins EGFP and mEGFP. Many of these features...the gene, the name of its gene product, and a description of its function. You can also click one location...

...plasmid encodes a green fluorescent protein gene which makes the bacterium fluoresce green. Comic by Maya...

... Lab’s recent Nature Biotechnology paper, they describe TurboID and miniTurbo, which utilize non-toxic... money, and viruses at low concentrations often escape detection. The Alexander Green lab found a solution...

...simple LED light. Lighting up your gRNA with fluorescent dyes In a scientific world where it often seems...seems like everything glows, why not spread the fluorescence to your gRNAs? Dye labeling the RNA in your ...

...lentiviral packaging methods. The three components described above (envelope, packaging, and transfer) are ...nomenclature of the plasmid backbone is meant to be descriptive of the components found within the backbone. ...

... NEB's Resource describing Dam and Dcm Methylases Discovery of DpnI Review describing dam-/dcm- E. coli...

...immediately write a text document with a brief description of the directory contents, at a minimum. This...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ...

...diverges from previously discussed methods. As described above, you design two primers, each containing...vitro recombination. Across all modifications described in Trehan et al., REPLACR-mutagenesis’ median ...

... for over 10 years. Many successful scientists describe having a “posse” of mentors as one key to their...Ellen Daniell. In “Every Other Thursday,” Daniell describes a group of 7 women scientists who met every other...

...mentoring questions I set out to answer. First, I will describe some of the many ways you can approach finding...Watch for my upcoming mentoring post in which I describe how to organize a successful peer mentoring circle...

...in HeLa and MEF cells. For further reading on fluorescent microscopy techniques, the first author of this...El-Samad lab has deposited plasmids encoding fluorescent reporters and other proteins controlled by GAL4...

...molecular biology experiments was in the job description, but this skill is not just about reacting to...research non-academic careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined...

...talking about here? Many of the Fab fragments described above can be used for similar applications and...tissue-specific molecules identified by a double-label immunofluorescence technique for monoclonal antibodies.  J Histochem...

..., non-dividing cells.   Figure 2: DNA rescue and transduction of a conventional single-stranded...Full-length ssAAV vector genome of both polarities are rescued from the vector plasmid and individually packaged...

...prone to degradation – those are the words that describe RNA! Double stranded? Well, that’s just for DNA... and repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular...

...Systems Biology in November, Voigt and Alex Nielsen describe what’s possible when multi-input CRISPR/Cas genetic...many deposits to Addgene over the years. Can you describe the toolbox you are putting together?  Voigt: ...

...dividing and non-dividing cells. More detailed descriptions of the lentiviral elements and the 2nd and 3rd... deletion (Cre), together with expression of fluorescent or enzymatic reporters for cellular assays and...

...A recent publication [1] from the Sánchez lab describes the plasmids and protocols required for both WISH... which are available through the deposit) are described in the Sánchez lab publication [1] so that the...

...efficient. Informed by these mutations and previously described TadA mutations (Gaudelli et al., 2017), they modified...undesired off-target editing effects. ABEs have been described to introduce base changes in cellular DNA and ...