We narrowed to 711 results for: ESC
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TypeBlog Post...fantastic K12 education initiatives like BiteScis (https://bitescis.org/) and amazing graduate student writing...
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Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post... articles reporting chromatin-related research describe the production of plasmids that might be useful...colleagues and guest bloggers have written posts describing new methods and tools for studying chromatin ... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post... NEB's Resource describing Dam and Dcm Methylases Discovery of DpnI Review describing dam-/dcm- E. coli... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog Post...immediately write a text document with a brief description of the directory contents, at a minimum. This...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...diverges from previously discussed methods. As described above, you design two primers, each containing...vitro recombination. Across all modifications described in Trehan et al., REPLACR-mutagenesis’ median ... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog Post... for over 10 years. Many successful scientists describe having a “posse” of mentors as one key to their...Ellen Daniell. In “Every Other Thursday,” Daniell describes a group of 7 women scientists who met every other... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post...mentoring questions I set out to answer. First, I will describe some of the many ways you can approach finding...Watch for my upcoming mentoring post in which I describe how to organize a successful peer mentoring circle... -
Antibodies 101: Fab Fragments
TypeBlog Post...talking about here? Many of the Fab fragments described above can be used for similar applications and...tissue-specific molecules identified by a double-label immunofluorescence technique for monoclonal antibodies. J Histochem... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post..., non-dividing cells. Figure 2: DNA rescue and transduction of a conventional single-stranded...Full-length ssAAV vector genome of both polarities are rescued from the vector plasmid and individually packaged... -
All in a Twist: dsRNA
TypeBlog Post...prone to degradation – those are the words that describe RNA! Double stranded? Well, that’s just for DNA... and repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...molecular biology experiments was in the job description, but this skill is not just about reacting to...research non-academic careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...in HeLa and MEF cells. For further reading on fluorescent microscopy techniques, the first author of this...El-Samad lab has deposited plasmids encoding fluorescent reporters and other proteins controlled by GAL4... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog Post...simple LED light. Lighting up your gRNA with fluorescent dyes In a scientific world where it often seems...seems like everything glows, why not spread the fluorescence to your gRNAs? Dye labeling the RNA in your ... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...Systems Biology in November, Voigt and Alex Nielsen describe what’s possible when multi-input CRISPR/Cas genetic...many deposits to Addgene over the years. Can you describe the toolbox you are putting together? Voigt: ... -
CRISPR in the Clinic
TypeBlog Post...potential for treatment is no longer an accurate descriptor; progress in clinical therapy is more fitting...They found that the cataracts phenotype could be rescued by zygote co-injection of Cas9 and a single-guide... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...efficient. Informed by these mutations and previously described TadA mutations (Gaudelli et al., 2017), they modified...undesired off-target editing effects. ABEs have been described to introduce base changes in cellular DNA and ... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab deposited AAV...promoter to drive expression of a Cre-dependent fluorescent CatCH-GFP reporter. Expression of GFP will only... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...A recent publication [1] from the Sánchez lab describes the plasmids and protocols required for both WISH... which are available through the deposit) are described in the Sánchez lab publication [1] so that the... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...dividing and non-dividing cells. More detailed descriptions of the lentiviral elements and the 2nd and 3rd... deletion (Cre), together with expression of fluorescent or enzymatic reporters for cellular assays and... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post...for binding to the target protein. The protocol describes going through two rounds of phage display to increase...interest. The Seeger lab used the ELISA technique described in their protocol because it is less susceptible...