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  1. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...immediately write a text document with a brief description of the directory contents, at a minimum. This...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ...

  6. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...talking about here? Many of the Fab fragments described above can be used for similar applications and...tissue-specific molecules identified by a double-label immunofluorescence technique for monoclonal antibodies.  J Histochem...

  7. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ..., non-dividing cells.   Figure 2: DNA rescue and transduction of a conventional single-stranded...Full-length ssAAV vector genome of both polarities are rescued from the vector plasmid and individually packaged...

  8. All in a Twist: dsRNA

    Type
    Blog Post
    ...prone to degradation – those are the words that describe RNA! Double stranded? Well, that’s just for DNA... and repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular...

  9. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...simple LED light. Lighting up your gRNA with fluorescent dyes In a scientific world where it often seems...seems like everything glows, why not spread the fluorescence to your gRNAs? Dye labeling the RNA in your ...

  10. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...molecular biology experiments was in the job description, but this skill is not just about reacting to...research non-academic careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined...

  16. CRISPR in the Clinic

    Type
    Blog Post
    ...potential for treatment is no longer an accurate descriptor; progress in clinical therapy is more fitting...They found that the cataracts phenotype could be rescued by zygote co-injection of Cas9 and a single-guide...

  17. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...visualize proteins in vivo by tagging them with fluorescent proteins to study localization or purify proteins...Expression Systems Recombinant protein expression in Escherichia coli: advances and challenges Production of recombinant...

  20. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...Building on the lab’s previous work, Sakuma et al. describe a detailed protocol for MMEJ-mediated knock-in... transgenes. It’s important to note that the fluorescence level observed will be dependent on both the...
Showing: 341 - 360 of 703 results