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  1. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...standard FPs. The Looger Lab was thus able to easily perform a four-color labeling experiment using smFPs and...References 1. Viswanathan, Sarada, et al. "High-performance probes for light and electron microscopy." Nature...

  4. Five Popular Model Organisms

    Type
    Blog Post
    ... can also be easily knocked-down through RNA interference (RNAi) by feeding the worm bacteria expressing... Andrew, et al. "Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans...

  5. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...molecular processes that make aptamer biology a powerful tool. In this post, we’ll take a deeper dive into... Matthew D. Disney, and Samie R. Jaffrey. "A superfolding Spinach2 reveals the dynamic nature of trinucleotide...

  7. Retrovirus Guide

    Type
    Guide
    ... in gene therapy research to reduce promoter interference and/or drive high expression of a transgene ...

  8. A Control for All Seasons

    Type
    Blog Post
    ...extremely bright, then the noise likely will not interfere. If the fluorescence from your target is weak,...fluorescence of the sample, or autofluorescence, also interferes with data interpretation and can be addressed...

  9. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...more.  Conclusion   Optogenetic tools provide a powerful way to study multiple aspects of cellular activity...important to understand the details of how each tool performs and the consequences of off-target activity. Whether...

  10. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...caught the attention of researchers wanting to perform whole-animal imaging, however NLuc’s emission peak...higher the emission from the acceptor, the more powerful the FRET signal. In well-designed FRET-based biosensors...

  11. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...polymerase is a phage-derived polymerase that can perform whole genome amplification (by a mechanism known...below). This initial transformation should be performed using a highly competent strain that you can use...

  12. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...produce completely transgenic offspring. Although powerful, this technology is cumbersome to use, not that...concept, straightforward to use, and robust in performance. In its natural setting, CRISPR-Cas9 is an acquired...

  13. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...ever both to engineer specific DNA edits and to perform functional screens to identify genes involved in...chromatin status). No computational prediction is ever perfect, but this can decrease the number of guides one...

  14. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...for guiding new deposits into the repository, performing quality control, and providing technical customer...strong sense of purpose, an excellent leader, and perfectly clear lines of communication, but if teammates...

  15. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... questions one could pursue and experiments to perform. It is tempting to pursue a new line of inquiry... school student. Learn to teach how to design, perform, and interpret experiments. You'll be better prepared...

  16. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...link genotype and phenotype. It is also key to perform these validations as translational researchers ...the Addgene Blog Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse ...

  19. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...restriction enzymes (Weber et al., 2011). It can be performed at multiple levels to generate anything from individual... tags, connectors, cDNAs, and more to find the perfect combination. The EcoFlex Kit has a wide array of...
Showing: 341 - 360 of 631 results