RNA Extraction Without A Kit
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Blog Post
...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...
We narrowed to 444 results for: HAL;
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CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post... with Cas9 guided by different promoters. The challenge in building genetic circuits is getting enough... -
Plasmids 101: Cre-lox
TypeBlog Post...in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology,... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...pronucleus of the zygote which is both technically challenging and can negatively impact zygote viability. Nanoblades... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post...the injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...students (1, 2), but educators face significant challenges in designing suitable projects. In the O'Connor... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...codon-optimized cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes a ... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog Post... make it difficult to detect proteins with short half-lives. Interested in using Split-BioID with your... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post... (budding yeast), S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus (rabbit), synthetic... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post... is only ~70kDa, with a molecular weight roughly half of Cas9 or Cas12a. This is in part due to the fact... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post...Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church ... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...that have the gene deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. ... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting. Front Plant Sci. 2015;6. ... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post...symptoms, including the development of fetal microcephaly and Guillain-Barré syndrome. In response, the... -
The Materials Science of Optogenetics Experiments
TypeBlog Post...validation experiments we discussed last week are only half of the battle when using optogentics to answer a... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post... case. Funding Makerspaces One of the main challenges when starting a new project at a makerspace is... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post...technologies currently available, you now face the challenge of trying to figure out what system might work...