...because I knew it would make me a better writer, but also because I knew it would make me a better scientist...mates, you add the individual figures and results to make the final product with an interesting introduction...

... mechanisms for feedback (more on this below). Make sure to give positive feedback when it is merited...some people find this hard so get used to it by making it a concrete goal to say “thank you, job well ...unnecessarily big issue for you and for the employee. Make sure to focus on the action, not the person - intent...

... of the features you may want to consider as you make your choice. The first and most important thing ...vector that you will need. You will also have to make sure that your plasmid has been incorporated into... applications used to study gene function. From making transgenic animals, gene targeting and genome modifications...

... of bacterial genomes utilize this system (4). Makarova et al. grouped the systems into three distinct...Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata A. J Bacteriol. 1987 Dec; ...1709–1712 (2007). CRISPRdb. Date: 2013-09-18. Makarova K, Haft DH, Barrangou R, Brouns SJJ, Charpentier...

... four. No Y chromosome is present in this line, making it genetically female. HEKs are generally cultured...properties. HEK-293 cells are tumorigenic, which makes them a candidate cell line model for cancer-specific...reproducible results. It’s also widely available, which can make for a seamless transition of biological tools to...

...spatio-temporal regulation, and is difficult to reverse, making it unsuitable for some types of experiments. (To...arabinose, requires many wash steps to remove arabinose making it difficult to regulate quickly. Arabinose also...Khammash M, Di Ventura B (2021) Engineering AraC to make it responsive to light instead of arabinose. Nat...

...development, vaccine development, and assay technologies makes her a perfect fit for Addgene. She was Head of Quality...around the world who would otherwise have difficulty making or getting these reagents is profound.” Frank Stegmeier...we’ve built create inefficiencies that ultimately make innovation and science slow,” Vandenberghe says....

...battery bank and the vehicle battery/alternator to make sure the vehicle battery didn't run down. In...temperatures and unstable ground around these hot springs make them extremely dangerous, we used telescoping poles...protocol. IgnaviCas9’s incredible thermostability makes the enzyme particularly useful. Additional resources...

...screening experiments. Get the full details on how to make these libraries from the post and the associated...Editing CRISPR has revolutionized how researchers make knockouts and other types of genome edits, but, ...depositors and guest writers. Your contributions make Addgene and the Addgene Blog useful resources for...

...experiments, making AAV starts with some plasmids. You just need three plasmids to start making AAV:  the...sequence Inverted terminal repeats (ITRs) are what makes an AAV transfer plasmid an AAV transfer plasmid....

... A good rule of thumb is to find the pieces that make you think, “Oh, that’s interesting! How did you ...in with something that sparks their interest and makes them want to know more. This could be by outright...needs to capture your audience’s attention enough to make them decide to read (or listen or watch) further...

...properties make nanobodies easier to transform and express in bacteria, which, in turn, makes them more...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

...resolution functional tracking of T lymphocytes, too, making them a versatile tool for application in both neuroscience...element-binding protein (CREB) in living cells. “Now we are making this really good,” Griesbeck said. “We know CREB... but not the other). “It’s quite a robust way to make a FRET sensor with a large dynamic range,” he says...

...researchers to utilize and develop these tools by making their own fluorescent protein technologies widely...off with light.  Xin hypothesized that she could make enzymes controllable by tethering them to Dronpa...communication skills throughout her training and wants to make her work understandable to people both inside and...

...express YFG in a new tissue type (more details on making a fly line in the next blog post). For example,...get the right cell type expression as opposed to making a new fly with hedgehog under a new promoter each... are already available, sometimes you’ll have to make your own. In my next post, I’ll explain the relatively...

...greater familiarity with scientific methodology makes it easier to spot even minor weakness or limitations...Zealand’s multiple Covid-19 lockdowns, I held mask-making workshops in my community. I brought along printed...run workshops where I showed people how they can make reusable cloth menstrual pads while we talked about...

...Professor at University of Barcelona. The challenge: Making courses on genetic manipulation more hands on While...University of Barcelona wanted to develop strategies to make our classes more practical and hands-on. Students...interactive setting. The problem is…how can you make a “hands-on” lesson on genetic manipulation of human...

... of making science easier and more transparent. That’s largely my job [at the Broad] too… to make science...help guide and promote Addgene in its mission to make research easier. Addgene is excited to announce ...

... Therefore, it is not always mission critical to make sure the plasmid replicates in the cell, as many... (i.e. the plasmid contains a cassette that will make cells resistant to a toxin), whereas negative selection... (i.e. the plasmid contains a cassette that will make cells sensitive to a toxin). In the table below ...

.... Before you read out your plots, you’ll want to make sure you’ve also adjusted voltage and compensated...tips on adjusting voltage: During data acquisition, make sure that the signal for the positive population... dot plot in Figure 2. Quantification using MFI makes it easier to identify and study these subtle shifts...