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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 807 results for: MAK;

Showing: 341 - 360 of 807 results

  1. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...partners and Tech Transfer Offices at universities to make COVID-19 research-related plasmids available more...Find plasmids available to industry scientists To make plasmid sharing more open for academic and industry... deposited plasmids at Addgene and would like to make them available to industry, please reach out at ...

  2. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ... by making them brighter, more photostable, smaller, and less toxic to cells. They’d like to make near-infrared... muscles work inside a living animal. For those making use of these probes, he says, when in doubt, read...

  3. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...toxicity or high off target effects, both of which make it a non-ideal drug. If a drug selectively kills...added QC is where CRISPR can save time and money to make sure quality candidates are selected.      ...Additional resources on the Addgene blog CRISPR 101: Making a Knock-In Cell Line Using CRISPR-Cas9 to Edit...

  4. Fostering communication in remote collaborations

    Type
    Blog Post
    ...best way to keep everyone on the same page is to make communication a non-negotiable part of your collaboration...Luckily, there are techniques and tools that helped make my experience run smoothly and can help other teams...meetings can be tricky, and it takes extra effort to make sure everyone in the meeting has an equal opportunity...

  5. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...because I knew it would make me a better writer, but also because I knew it would make me a better scientist...mates, you add the individual figures and results to make the final product with an interesting introduction...

  6. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ... mechanisms for feedback (more on this below). Make sure to give positive feedback when it is merited...some people find this hard so get used to it by making it a concrete goal to say “thank you, job well ...unnecessarily big issue for you and for the employee. Make sure to focus on the action, not the person - intent...

  7. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ... of the features you may want to consider as you make your choice. The first and most important thing ...vector that you will need. You will also have to make sure that your plasmid has been incorporated into... applications used to study gene function. From making transgenic animals, gene targeting and genome modifications...

  8. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ... of bacterial genomes utilize this system (4). Makarova et al. grouped the systems into three distinct...Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata A. J Bacteriol. 1987 Dec; ...1709–1712 (2007). CRISPRdb. Date: 2013-09-18. Makarova K, Haft DH, Barrangou R, Brouns SJJ, Charpentier...

  9. What the HEK?

    Type
    Blog Post
    ... four. No Y chromosome is present in this line, making it genetically female. HEKs are generally cultured...properties. HEK-293 cells are tumorigenic, which makes them a candidate cell line model for cancer-specific...reproducible results. It’s also widely available, which can make for a seamless transition of biological tools to...

  11. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...experiments, making AAV starts with some plasmids. You just need three plasmids to start making AAV:  the...sequence Inverted terminal repeats (ITRs) are what makes an AAV transfer plasmid an AAV transfer plasmid....

  12. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ...spatio-temporal regulation, and is difficult to reverse, making it unsuitable for some types of experiments. (To...arabinose, requires many wash steps to remove arabinose making it difficult to regulate quickly. Arabinose also...Khammash M, Di Ventura B (2021) Engineering AraC to make it responsive to light instead of arabinose. Nat...

  13. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ... A good rule of thumb is to find the pieces that make you think, “Oh, that’s interesting! How did you ...in with something that sparks their interest and makes them want to know more. This could be by outright...needs to capture your audience’s attention enough to make them decide to read (or listen or watch) further...

  14. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...development, vaccine development, and assay technologies makes her a perfect fit for Addgene. She was Head of Quality...around the world who would otherwise have difficulty making or getting these reagents is profound.” Frank Stegmeier...we’ve built create inefficiencies that ultimately make innovation and science slow,” Vandenberghe says....

  16. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...screening experiments. Get the full details on how to make these libraries from the post and the associated...Editing CRISPR has revolutionized how researchers make knockouts and other types of genome edits, but, ...depositors and guest writers. Your contributions make Addgene and the Addgene Blog useful resources for...

  17. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...resolution functional tracking of T lymphocytes, too, making them a versatile tool for application in both neuroscience...element-binding protein (CREB) in living cells. “Now we are making this really good,” Griesbeck said. “We know CREB... but not the other). “It’s quite a robust way to make a FRET sensor with a large dynamic range,” he says...

  18. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...researchers to utilize and develop these tools by making their own fluorescent protein technologies widely...off with light.  Xin hypothesized that she could make enzymes controllable by tethering them to Dronpa...communication skills throughout her training and wants to make her work understandable to people both inside and...

  20. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...greater familiarity with scientific methodology makes it easier to spot even minor weakness or limitations...Zealand’s multiple Covid-19 lockdowns, I held mask-making workshops in my community. I brought along printed...run workshops where I showed people how they can make reusable cloth menstrual pads while we talked about...
Showing: 341 - 360 of 807 results