We narrowed to 775 results for: Mos

...engineering tool. It is easy to use, functional in most species, and has many applications. That said, SpCas9...terms of structure, Cas13a shares no homology to the most commonly used CRISPR enzymes - Cas13a contains two...specifically targeted RNAs. Finally (and perhaps most interesting), Cas13a’s propensity to cleave any ...

...fluorescent visualization techniques for decades, most notably for fluorescent in situ hybridization (FISH...readout may render this approach less sensitive in most applications. Learn About Fluorescent Biosensors...Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc. Natl. Acad. Sci. ...

...block of time was. Eventually, I learned that I was most productive in short bursts (even twenty minutes ...minutes was enough to make some progress). For most researchers, it’s not always possible to pull a Thoreau ...useful data Typically lists and pictures are the most useful parts of reviews. These could be in the form...

... exist for reducing Cas9 off-target effects, but most studies have focused on the off-target cutting of...not associated with ricin regulation, including almost all genes involved in the production of a cell ...fail to identify some known ricin-regulators, but most of these genes are also essential for growth and...

...much easier to map these neuronal networks. For almost three decades, recombinant Adeno-associated virus...IHC) experiments with tag-specific antibodies. The most famous ones are influenza hemagglutinin (HA), myelocytomatosis... microscopes, but they can also be used with the most cutting-edge imaging technologies such as array ...

...the fly community. Not surprising, this is where most of our conference room names originated from. Let...that contain SHH, you can find them all here! The most popular SHH plasmid however was deposited by none...insert specific pieces of DNA into vertebrate chromosomes via a cut and paste mechanism. Scientists reconstructed...

... just creating, which for many scientists is the most enjoyable aspect of their chosen career. You can...’t know what it’s like to be properly onboarded. Most work environments outside of academia provide a ...skills you pick up wherever you work, and you’ve almost certainly developed some in the lab whether you...

...markers are set up sequentially, from broadest to most specific marker, and are represented as a series...quadrant will often be too clean for real-world data. Most plots will have a shape, like a box, ellipse, or...  As the gates progress from broadest markers to most specific, the first step is to separate cells from...

...your best to show up as your most prepared, can-do self and make the most of it! So, what can you do ...dynamic and set yourself up for success! Getting the most out of the training experience In research there...

...Planning makes perfect Our first tip, and arguably the most important, is to take some time to sit down, plan...assemblies in silico before you start doing your cloning. Most sequence analysis software includes Golden Gate ...kit has just arrived! MoClo kits from Addgene will most likely arrive in your laboratory as plates of frozen...

...adenine transversion editors Uracil excision is the most common type of base excision repair. But mammals... retained considerable A → G editing activity at most sites, in addition to A → C and A → T activity. ... base from DNA to create an abasic site. Like in most other base editor designs, the Cas9 nicks the non-edited...

... is reinforcing, the animal will prefer to spend most of its time in the chamber in which it receives ...conversely, if the circuit is aversive, it will spend most of its times in the opposite chamber) [2]. Laser...standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this species. However...

...types and often results in biallelic modifications. Most commonly, DSBs are repaired by the nonhomologous...associated with dementia, such as APP and PSEN1, in most cases the intended HDR events were corrupted by ...’t get what we wanted. The Indels were gone from most HDR reads that we studied by deep sequencing, but...

...Microscopy (University of Amsterdam). GFP is the most popular, most widely used genetically encoded fluorescent...maturation to functional, fluorescent protein in almost all organisms and cell types, (ii) no need to add...

... the company. I’d identified the ways to get the most out the resources we have, and we developed a great...But there were still many restrictions in place, most importantly, masks.  Besides the muffled voices ....  Test: Our first official shoot went off with almost no hitches. The wheeled stand was a bit cumbersome...

... extracellular environment and the cytoskeleton. Most integrins have three conformational states: bent-closed...many function-blocking integrin antibodies exist, almost all bind outside the ligand-binding pocket, their...challenging the long-held belief that αVβ3 would be the most functionally important integrin on these cells. ...

...microtubule-cutting sword Microtubules are one of the most important components of the cytoskeleton. They provide...provide cells with physical structure, separate chromosomes during mitosis, support transport of organelles...adhesion But what if you’re interested in that other most-important component of the cytoskeleton – actin?...

...but its effectiveness varied depending on the chromosomal location, sequence context, and cell type. Multiple...editor. For this application, ABE8e turned out to be most effective. Targeting the right PAM Expanding PAM... large number of gRNAs could be screened for the most efficient editing. In these initial experiments,...

...side of things that have the most utility; i.e. those things that will be most useful for achieving your ...finish any additional work you might need to do. In most cases, I would recommend the second option for the...

...previously appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications... A number of classic methods exist for this, the most common of which is the utilization of phosphomimetic...are available through Addgene, but we have worked most extensively worked with and optimized Sep-OTSλ and...