We narrowed to 391 results for: NAM

1. Supporting Reproducibility with a Connected ELN

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   ...static and isolated vehicle and becomes part of the dynamic and connected record of research. In RSpace, document...

2. To Codon Optimize or Not: That is the Question

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   ...2006) and mRNA stability (Hausser et al., 2013) to name a few. This could affect synonymous mutations which...

3. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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   ... of near-PAMless Cas9 variants. These variants, named SpG and SpRY, can target NGN PAMs and NRN and NYN...

4. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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   ...relying solely on GFP (Katti et al., 2020). They named this reporter “Gene On” or GO. GO works by affecting...

5. Finding nucleic acids with SHERLOCK and DETECTR

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   ...modifications to improve their detection system. They named this system SHERLOCKv2 for SHERLOCK version 2. Here...

6. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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   ... networks The last stage is Exponential Growth, named after the growth phase when bacteria in favorable...

7. New CRISPR Tools: Cas7-11 and PASTE

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   ... DNA yielded a new CRISPR effector protein they named Cas7-11. Testing showed that Cas7-11 cut RNA with...

8. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   ... Table 1: Comparison on Common CRISPR Enzymes Name  Enzymatic Domains Guide RNA Target PAM (PFS) Cleavage...

9. Plasmids 101: Shuttle Vectors

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   ...aminoglycosides work on different host species: kanamycin targets the prokaryotic ribosome, while G418 (...

10. Antibodies 101: The Four ELISAs and When to Use Them

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    ...method are similar to those of the direct method. Namely, since the assay requires a single conjugated primary...

11. How to Be an Excellent Trainee

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    ...can do a lot to contribute to the relationship dynamic and set yourself up for success! Getting the most...

12. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    ...hypothetical protein, improving expression, and named this gene set bARGSer, for bacterial ARG from Serratia...

13. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    ...and how does it work? FLP recombinase, which is named after its ability to invert or ‘flip DNA’, is derived...

14. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    ...researchers to investigate the localization and dynamics of fluorescent protein fusions in live cells in...

15. Important Considerations in Optogenetics Behavioral Experiments

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    ...methods 9.2 (2012): 159-172.5. Goshen I, et al. Dynamics of retrieval strategies for remote memories. Cell...

16. Plasmids 101: The Promoter Region – Let's Go!

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    ... the human ubiquitin C gene Constitutive  As the name implies, this promoter is ubiquitous. human beta...

17. Five Popular Model Organisms

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    ...cell signaling, aging, and mechanotransduction to name a few. For example, Andrew Leifer’s lab utilized...

18. March for Science

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    ...science in government: 314 Action: This nonprofit named for the mathematical constant “pi” supports scientists...

19. Antibodies 101: Beyond Surface Labeling

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    ...think of mummification). Permeabilization, as the name suggests, perforates the cell membrane to allow ...

20. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    ...efficiency of the original APEX (Lam et al. 2014). They named this mutant APEX2.  Find the APEX2 plasmids! TurboID...