We narrowed to 403 results for: NAM

1. Droplet Digital PCR for AAV Quantitation

   Type

   Blog Post

   ...diluting the virus. It is important to note that the dynamic range of the ddPCR is between 1 and 100,000 genome...

2. Better Dyeing Through Chemistry & Small Molecule Fluorophores

   Type

   Blog Post

   ... D. Lavis, and Eric Betzig. "Imaging live-cell dynamics and structure at the single-molecule level." Molecular...

3. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

   Type

   Blog Post

   ...3D fractal). Enhancer biology is a complex and dynamic field. We’re going to focus on a tried and true...

4. Cpf1: A New Tool for CRISPR Genome Editing

   Type

   Blog Post

   ...potentially overcome some of Cas9’s shortcomings - namely its blunt double stranded cleavage and G-rich PAM...

5. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

   Type

   Blog Post

   ...engineered DNA-binding molecules for the first time and named the new technology as engineered DNA-binding molecule-mediated...

6. QC Sequencing Technologies at Addgene

   Type

   Blog Post

   ...synthesis-based sequencing methods (Figure 3). It uses (aptly named) “nanopores” that are attached to the flow cell ...

7. Plasmids 101: Shuttle Vectors

   Type

   Blog Post

   ...aminoglycosides work on different host species: kanamycin targets the prokaryotic ribosome, while G418 (...

8. New CRISPR Tools: Cas7-11 and PASTE

   Type

   Blog Post

   ... DNA yielded a new CRISPR effector protein they named Cas7-11. Testing showed that Cas7-11 cut RNA with...

9. To Codon Optimize or Not: That is the Question

   Type

   Blog Post

   ...2006) and mRNA stability (Hausser et al., 2013) to name a few. This could affect synonymous mutations which...

10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    ... of near-PAMless Cas9 variants. These variants, named SpG and SpRY, can target NGN PAMs and NRN and NYN...

11. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type

    Blog Post

    ...relying solely on GFP (Katti et al., 2020). They named this reporter “Gene On” or GO. GO works by affecting...

12. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    ...modifications to improve their detection system. They named this system SHERLOCKv2 for SHERLOCK version 2. Here...

13. How to Be an Excellent Trainee

    Type

    Blog Post

    ...can do a lot to contribute to the relationship dynamic and set yourself up for success! Getting the most...

14. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    ...and how does it work? FLP recombinase, which is named after its ability to invert or ‘flip DNA’, is derived...

15. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    ... Table 1: Comparison on Common CRISPR Enzymes Name  Enzymatic Domains Guide RNA Target PAM (PFS) Cleavage...

16. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    ...method are similar to those of the direct method. Namely, since the assay requires a single conjugated primary...

17. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type

    Blog Post

    ...hypothetical protein, improving expression, and named this gene set bARGSer, for bacterial ARG from Serratia...

18. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    ... networks The last stage is Exponential Growth, named after the growth phase when bacteria in favorable...

19. Supporting Reproducibility with a Connected ELN

    Type

    Blog Post

    ...static and isolated vehicle and becomes part of the dynamic and connected record of research. In RSpace, document...

20. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type

    Blog Post

    ...researchers to investigate the localization and dynamics of fluorescent protein fusions in live cells in...