We narrowed to 423 results for: NUC

1. Getting to Know Expansion Microscopy

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   ...with SRM techniques, and adapted for detecting nucleic acids as well as proteins (Klimas, et al. 2023;...

2. Plasmids 101: Protein tags

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   ... with a tag that localizes your protein to the nucleus? Or you want multiple rounds of purification to...

3. Your Lentiviral Plasmid FAQs Answered

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   ...retroviruses. Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing ...

4. New Tools Enable CRISPRa for Neuroscience Applications

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   ...CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations...

5. Neuronal labeling with Spaghetti Monster

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   ...Retrograde AAV: Making the journey from axon to nucleus AAVs CREATEd for gene delivery to the CNS and PNS...

6. Getting the Most from Your Lentiviral Transduction

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   ...the cell cycle; quiescent cells do not take up nucleic acid or express transgenes as well as actively ...

7. Bioinformatics at Addgene

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   ...have accumulated more than 300,000 individual nucleotide sequences. More than half of these sequences ...

8. Antibodies 101: Reading a Flow Plot

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   ...scatter roughly measures granularity, a proxy for nucleic complexity of the cell.  The sample will have been...

9. Hot Plasmids - November 2023

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   ... able to label proteins moving to and from the nucleus, ER, mitochondria, stress granules, and more. For...

10. Viral Production at Addgene

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    ...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for...

11. Plasmids 101: Stringent Regulation of Replication

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    ...DnaA-ATP in initiation of chromosome replication. Nucleic Acids Research, 46(12), 6140–6151. https://doi....

12. Plasmids 101: Broad Host Range Plasmids

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    ...Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2. Molecular...

13. Mesothelioma - Causes, Symptoms, and Treatment

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    ...group box 1 protein. Normally localized to the nucleus, if the cell dies from injury, this protein leaks...

14. Important Considerations in Optogenetics Behavioral Experiments

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    ... "Excitatory transmission from the amygdala to nucleus accumbens facilitates reward seeking." Nature 475.7356...

15. Plasmids 101: The Promoter Region – Let's Go!

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    ...small RNA expression shRNA From the human U6 small nuclear promoter Constitutive  Murine U6 is also used, ...

16. CRISPR Plasmids - dCas9-FokI

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    ...catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes, it generates a double-strand...

17. iPSC Neurodegenerative Disease Initiative Plasmid Collection

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    ...project aims to generate and characterize single nucleotide variant lines in an isogenic cellular background...

18. CRISPR Plasmids - CRISPR Transposases (CAST)

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    ... at specific locations. The Cas effector lacks nuclease activity, instead recruiting the transposase (...

19. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    ...to specific subcellular locations, such as the nuclear envelope, allowing scientists to probe interactions...

20. Five Popular Model Organisms

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    ...like those that overexpress the toxic protein α-synuclein (the most common protein found in lewy bodies)...