We narrowed to 766 results for: Ncl

...are multiple methods of DNA assembly available, including: Gibson Assembly®, BioBrick® Assembly, Golden ...applications of the NEBuilder HiFi DNA Assembly Mix include:  Generation of constructs with multiple point...

...symposium, a panel of thought leaders in the field--including Shinya Yamanaka, George Q. Daley, Christine Mummery... promising clinical trials for ALS.   I have an uncle with Parkinson's, a half-brother who is a quadriplegic...

...researchers explore many aspects of ApoB-LP biology, including quantification of abundance, size, and localization...made these constructs available at Addgene. These include Arch(D95N) and QuasAr, as well as the eFRET sensors...

...that can influence the HDR/NHEJ decision. Our conclusions reinforce a key scientific principle: that understanding...frequencies of genome cutting and, when donor DNA is included in the nucleofection reaction, extremely high ...

...plasmid on the right has a total size of 7.3kb, including a 1.2 kb insert. The plasmid was digested with...run on an agarose gel. The resulting gel image includes a 1kb ladder (lane 1) that has bands ranging from...

... Tom Ellis was offered a start-up package that included a postdoc and a PhD student in his first year.... great resources for Early Career Scientists, including their "Making the Right Moves: A Practical Guide...

...plasmids differ from their bacterial counterparts, including how replication occurs and whether selection is...in the cell, as many of these experiments are concluded within 48 h of transfection. Of course, it is ...

... is not fluorescence! Chemiluminescence, which includes all bioluminescence, is the production of light...Promega to offer their Promega Plasmid Collection, including tools using NanoLuc®, NanoBiT, and more. Which...

...backbone to generate R-mAbs of different subclasses, including subclasses that were not produced by the original...expanded their recombinant affinity reagent tools to include nanobodies. At Addgene, we are excited to be part...

...controls are used in antibody-based applications including flow cytometry, immunohistochemistry, immunocytochemistry...region length. Now for some essential isotype nomenclature. Antibody heavy chain proteins as well as the...

...generate antibodies against many types of molecules including proteins, lipids, and carbohydrates, in this blog...2005). This is reflected in the antibody’s cost. Conclusion Whether you choose to use a monoclonal antibody...

...organisms in which it was previously not an option, including Drosophila and C. elegans.   Find the TurboID ...that one might perform using the purified domains include protein stability, small molecule binding screens...

...Reis e Sousa from the Francis Crick Institute concluded “Aspirin promotes autophagy, inhibits platelet...field of Immuno-Oncology, a sizzling hot topic, includes studies on novel cancer therapeutics such as  ...

...nano-lanterns for use in various applications, including optogenetics, biosensors, and fusion proteins....al. expanded the Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these...

...information on each cancer clinical sequencing report includes rare variants that haven’t been characterized?...the scientific community through Addgene. These include 634 sequence confirmed mutant cDNA clones corresponding...

...expression of other endonucleases, like Csy4, or the inclusion of processing signals, such at tRNAs, to process...its own pre-crRNA arrays eliminates the need to include multiple promoters to drive crRNA expression and...

...Other examples of chemically induced promoters include positive inducible alcohol and steroid regulated... be induced by heat or cold exposure. Examples include the heat shock-inducible Hsp70 or Hsp90-derived...

...sociology, behavior, and artificial intelligence (including a team that created a novel form of AI that can...require large data sets. Whether these data sets include videos of behavior, many many images of brain regions...

...The information is organized in wiki format and includes biochemical properties, previously established...to browse entries by the current phylogenetic nomenclature and discover orthologs with similar properties...

...shares many common features with gamma-retrovirus including genome architecture, host integration, and its...its cargo, in fact its viral life cycle does not include genomic integration. While the expression of adenovirus...