We narrowed to 663 results for: PPL;
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TypeBlog Post...reached at high power may not translate to real applications in which far less power is used. For example...insight into the photostability in the intended application (Goedhart et al, 2012). The third issue relates...
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Transferable Skills Guide: Cross-team Communication
TypeBlog Post...types or purposes of each given container, when applicable Any other relevant information Prescribing an...potentially ambiguous terms. Defining words and applying annotation can be particularly useful in academic... -
Antibodies 101: Secondary Antibodies
TypeBlog Post...IgG2 or IgG4. This can be important for some applications. Assay use While secondary antibodies are useful..., the flexibility of their use varies across applications. Western blots, for instance, almost always ... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post... Haoyi Qiu He Tian Every two years, we seek applications for awards to early career scientists using ...virtual conference attendance. We also opened up applications to research technicians and undergraduate researchers... -
RNA Extraction Without A Kit
TypeBlog Post...plasticware is readily available from scientific suppliers and glassware should be treated with a DEPC solution...potentially complicate results of a downstream application such as quantitative PCR to assess gene expression... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post... out large regions of DNA and has anti-viral application potential as a result. Fast facts – PAM requirement... has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.). The main... -
Summer SciComm: Preprints
TypeBlog Post...poster or talk, or submitting patent or grant applications; to informal settings like lab meetings, social...listing “Submitted” articles in their CV or grant applications. A number of organizations (such as ASAPbio)... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...It’s at Fred Hutch.” And I hadn’t seen it. So I applied and I got it.” Science writing at Fred Hutchinson...internship similar to Susan’s NEI internship. Application information can be found here Find tips for using... -
Visualizing Protein Turnover In Situ
TypeBlog Post...proteins with FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid... observed with fluorescence microscopy. This application of AHA labeling is known as fluorescence noncanonical... -
CRISPRainbow and Genome Visualization
TypeBlog Post...gRNA binding a non-mammalian sequence (GAL4) was supplied. In addition to labeling repetitive telomeric...single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector. Figure 2: CRISPRainbow... -
Developing Lab Management Software for Biology
TypeBlog Post... is almost always worth it. Because labels are applied to containers and not the mushy, gooey contents...be!) bear in mind that your barcodes are being applied to the containers, not to the contents. Tightly... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog Post...derived from S. aureus. This means you can likely apply similar rational mutations to your Cas9 homolog ...may decrease worries about off target effects in applied and/or therapeutic settings. Will combining the... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...were so many interesting questions that we could apply [it to].” Using ClonTracer to study drug resistance...Novartis Oncology and is particularly interested in applying what we have learned about tumor heterogeneity... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...engineering fields. We’ll walk you through how to apply this precise and easy-to-use system to your cloning...different combinations, or other new and exciting applications, this system is an incredibly powerful tool ... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...it comes to targeting precision and practical application. If the CRISPR/Cas9 system is not effective for...lncRNAs with high specificity, particularly in applications requiring smaller delivery systems (Zibitt et... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post... for lightning-mediated gene transfer in soil. Applied and Environmental Microbiology, 67(8), 3440–3444...Bacteria:Their Characteristics and Potential Applications in Biotechnology. Critical Reviews in Biotechnology... -
Engaging with science and society at pgEd
TypeBlog Post...latest developments in genetics research, their applications and regulatory policies; keeping tabs on the...and who will be affected by the knowledge and applications generated from this research. At both a practical... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...Glycoimmunology group based at the Research Unit on Applied Molecular Biosciences (UCIBIO) focused on understanding...different Arg-mutant enzymes could be rescued by supplementation with guanidine hydrochloride (GdnHCl). Rescue... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...of xanthine into guanine, and without xanthine supplied in the media, these bacteria are unable to divide...was able to grow this strain in minimal media supplemented with various caffeinated beverages, but not ... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...potential to be used for other types of CRISPR applications. Let’s take a look at the key components of ...After Gag-Cas9 is incorporated, the MLV protease, supplied as part of the MLV Gag-Pol polyprotein, cleaves...