We narrowed to 1,006 results for: RING;

...by loss of protein during digestion, or masking of epitopes during sample anchoring and gel polymerization... Tillberg, et al. 2016). That being said, the anchoring, digestion, and expansion steps can impact the...occurs, due to a reduction in target concentration during expansion. One group has found that using Fab fragment...et al (2023) Magnify is a universal molecular anchoring strategy for expansion microscopy. Nat Biotechnol...

...where droplet digital PCR (ddPCR) comes in. AAV titering using quantitative PCR Before we dive into the... PCR is complete, the results are analyzed by comparing the threshold fluorescence of the viral DNA to...reasons, we are in the process of transitioning our titering method from qPCR to ddPCR. Droplet digital PCR...reference, meaning saving on reagents (and time)! AAV titering using droplet digital PCR Droplet digital PCR ...generator, you will have to be careful about transferring your droplets from the droplet generator to ...detection of microbes. Some applications include measuring viable probiotics and detecting circulating pathogens...

...optimization? Codon optimization is an approach in gene engineering to improve gene expression by changing synonymous...efficiency and thus protein expression without altering the sequence of the protein. As DNA synthesis ...tRNAs, including those that exhibit wobble base-pairing (a tRNA that can recognize multiple synonymous ...of translation. The ribosome may slow and pause during elongation which may actually be necessary for ... SA, Zhou W, Mauro VP (2005) An mRNA-rRNA base-pairing mechanism for translation initiation in eukaryotes...02)00362-x Stadler M, Fire A (2011) Wobble base-pairing slows in vivo translation elongation in metazoans...

...inteins. Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins...Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 339:819–823...Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann J, Staahl..., Grimm D (2014) CRISPR/Cas9-mediated genome engineering: An adeno-associated viral (AAV) vector toolbox...Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b for human genome editing. Nat... Mary Gearing...

...are syringe or vacuum infiltration. Syringe infiltration (D'Aoust et al., 2009): Usually a syringe without...contains a plasmid with a T-DNA (transfer-DNA) region. During infection this T-DNA region is transferred into...transformation and gene expression can be tracked. During a transient leaf transformation, the engineered...Transient expression of antibodies in plants using syringe agroinfiltration." Recombinant proteins from plants...

... You need to balance respectfully answering questions while steering the conversation back to your vision...proposal or a 10-year research plan. I suggest preparing for both types. Your research vision is your approach...proposal will distinguish between competing models. Comparing model A vs. B is more constructive than testing...vision. On the day of your chalk talk Bring your own fresh set of erasable markers. You’ll have the colors...and consider eating one before your chalk talk. Bring your own bottle of water. If possible, write down...

...But CRBN has been difficult to study in vitro, requiring either expressing it with additional cofactors...faster, with expression levels comparable to GFP during early embryogenesis (Figure 3). mScarlet-I3 also... through the middle of each embryo are shown, comparing GFP, wrmScarlet (mScarlet), and the new mScarlet3...proteins of interest) and mScarlet-I3 for scenarios requiring rapid maturation and prolonged imaging with minimal...high rate of spontaneous antibiotic resistance interfering with selection. Using an inducible Cas9 expression...to cause disruption of the target gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited strain...

...the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 is an enormously plastic tool and...activator by simply providing a new gRNA rather than engineering an entirely new protein. This makes it much cheaper...types of Cas9 activators, check out our blog post covering VP64, Suntag, SAM, and VPR. Worry less about off...the Wyss Institute for Biologically Inspired Engineering.     Alejandro (Alex) Chavez is a member of the...develop several popular CRISPR-based tools for altering DNA sequence or regulating gene expression.   ...Church GM (2015) Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach....

...briefing, to quick notes, to finances, to flights & ordering food - everything is covered.   So why are we ...folders in complex ways, there are solutions that bring more to the table in terms of data traceability ...researchers today generate vast amounts of digital data during their work. It is already becoming impossible to...to keep all their data in one place and avoid scattering intellectual property across various software...functionalities include comments, notifications, sharing files, assigning team members to certain tasks,...compliance requirements – e.g. 21 CFR part 11, HIPAA? Answering this question helps you evaluate whether the ELN...

...interested in measuring a population based on a fluorescent stain, you might be wondering which second ...types of scattering that occur when a cell is pulsed by the laser. Within each type of scattering, you also...to keep it short though, promise! FSC and SSC: scattering To digitally extract your cells based on their...cells stain positively for both markers. When comparing two markers simultaneously, we commonly describe...

...about the particular audience and venue before tailoring each presentation appropriately.  Find out who...many non-scientists be present?  Always ask the hiring manager or host for topic advice. An added bonus...figure out how to make it suspenseful. 4. Start preparing your talk early - like today Don’t wait until ... all graduate students and postdocs form peer mentoring groups to practice giving talks and getting feedback...always have a few extra slides in their back pocket. During your practice rounds, encourage your listeners ...down it is a great segue to talk about how the sharing model works. See if you can elicit helpful comments...

...important information for anyone considering a postdoc. In “Changing Labor Laws Bring Increased Postdoc Wages,...readership with over 75,000 views. Changing Labor Laws Bring Increased Postdoc Wages Our most popular blog post...Grad School Advice Part 2: Building Community Engineering the Plant Genome Using CRISPR/Cas9 Healthcare...Reddit for Science Communication Changing Labor Laws Bring Increased Postdoc Wages I hope you all have a great...

... what challenges remain in materials sharing, information sharing, and research reproducibility while ...service and support.  Alongside our material offerings, in 2022 we also rolled out an updated AAV Data...around the world can upload AAV validation data, sharing what works and, equally importantly, what doesn...commitment to supporting the research community through sharing science and distributing high-quality materials...

...relatively simple in C. elegans and bacteria. But CRISPRing typical mouse models, including creating Cre-dependent...Megamer service tested by Quadros et al. When delivering the ssDNA template and CRISPR machinery, Quadros...facilities for 13 projects, with most projects requiring only 40-60 zygotes to generate a correctly edited...conditional alleles by CRISPR/Cas-mediated genome engineering." Cell 154 (2013):1370-9. DOI: 10.1016/j.cell... a robust method for in situ germline genome engineering using CRISPR nucleases. Genome Biol. 19 (2018... Mary Gearing...

...Westerns are divided into two categories, native and denaturing. In a native western, the protein’s secondary...is separated through a matrix by charge. In a denaturing western, the protein is denatured to its primary... purpose of this blog, we will be focusing on denaturing westerns.  Separating a protein mix by size Sodium...The wet transfer process is very effective in transferring a broad range of protein sizes but takes hours...blocking the membrane  from binding to the antibodies during the stain. The ideal blocking buffer depends on...loading control when assessing protein abundance or comparing samples across lanes.  For a typical loading control...

...social media, you could be sharing your research or finding opportunities to bring science to the community...the same page with the potential PI in terms of mentoring style and expectations. Make sure you will enjoy...mantra and never forgot it. Usually when I see inspiring artists who are thinking about getting into the... the process down into steps and set aside time during the week to tackle each one.  Get the toughest ...

...year, graduate students and post-docs alike are wondering where the time has gone. Are we any closer to ...to graduating, publishing that key paper, or figuring out a career path? Many trainees are developing Individual...accomplishments, from learning new techniques to finally figuring out a tough experiment, sets a positive and encouraging...Good Manager Anyway Developing Transferable Skills During Science Training ... Mary Gearing...

.... pombe. The protocol is surprisingly simple, requiring only a PCR product containing the modifying sequence...affinity purification of protein complexes. John Pringle and Jürg Bähler have deposited a large collection...genome modification in yeast developed by Dr. Pringle’s former lab at UNC Chapel Hill. Bähler et al.(2...McKenzie A 3rd, Steever AB, Wach A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):943-51. Longtine MS,... DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):953-61. Note: Marcy...

...openness and sharing in science.  We’re excited to support the neuroscience community by sharing not only ...capsid proteins that determine the AAV serotype. During transduction, the viral capsid proteins bind to...enhancing anterograde transport efficiency, and engineering viral capsids that target specific cell types...science, we aim to accelerate the rate by which sharing speeds science.   Check out the precise neuronal...

...believing, as we do, that sharing speeds science.  This year, Chonnettia will be sharing more of her perspective...what we accomplished in 2023 and thrilled to be sharing our plans for 2024.  In 2023, Addgene distributed... of innovation and impact made possible by the sharing of tools for research: discovered and developed...you a healthy, productive, and thriving 2024, mirroring our own aspirations for Addgene. On behalf of ...