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We narrowed to 613 results for: SET

Showing: 341 - 360 of 613 results

  3. Of Myc and Men

    Type
    Blog Post
    ... the same target. In the 1980s, Munro and Pelham set out to address this issue by developing generic strategies...

  6. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...allow for two sets of microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette into the locus...protocol for MMEJ-mediated knock-in of a GFP-Puro cassette into a given locus, just upstream of a stop codon...microhomology to the insertion locus flanking the GFP-Puro cassette. Three double stranded breaks are necessary for...for knock-in: one on either side of the GFP-Puro cassette and one in between the 5’ and 3’ microhomologies...of PITCh. The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and 3' microhomology and PITCh-gRNA...creates two DSBs in the PITCh plasmid to release the cassette, and an insertion-locus-specific gRNA cleaves ...and 3’ microhomologies are added to the GFP-Puro cassette via PCR, and this construct is inserted into the...

  8. Weighing Reagents Protocol

    Type
    Protocol
    ...measurement. Hit the tare button (or zero button) to set the balance to zero. Pro-Tip If you’re weighing a...

  9. Hot Plasmids - August 2020

    Type
    Blog Post
    ...be useful for your studies.  Find the plasmids to set up your screening systems at Addgene! Schematic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...

  11. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... used successfully in preclinical and clinical settings for ocular conditions. However, AdV was abandoned...responsible for an autosomal recessive, severe, childhood-onset form of blindness name Leber’s Congenital Amaurosis... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and...

  12. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...mice CRISPRa has largely been limited to in vitro settings due to the large size of Cas9 activators, which...packaging limits (i.e., how many sgRNA expression cassettes will fit). Cre-dependent expression of the SPH...

  13. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...the day of your chalk talk Bring your own fresh set of erasable markers. You’ll have the colors you want...research? Do you have innovative tools? A novel dataset? Unique expertise? A novel hypothesis? Part of ...

  14. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ... the use of a given timer in many experimental settings. Monomeric fluorescent protein timers Since the... simplifies cloning and makes your experimental setup less cumbersome. 3. Portability across multiple ...

  15. MXS Chaining

    Type
    Blog Post
    ...sequences of the genetic elements may differ (e.g., a set of various mammalian promoters), the same cloning...sequences. Intermediate chaining products (called “cassettes”) can be readily reused in the assembly of any...any later construct (i.e.,functional cassettes are recyclable) Can only work if the restriction sites used...

  16. Bioinformatics at Addgene

    Type
    Blog Post
    ...working on one process at a time, and testing a small set of data each time a new process was added, we progressively...easily shared with others without any complicated setup. We created, tested, and debugged each of our pipeline...

  18. Hot Plasmids - November 2023

    Type
    Blog Post
    ...This modular approach combines an interchangeable set of fusogenic and targeting components to reprogram...Every few months, we highlight a subset of the new plasmids, antibodies, and viral preps in the repository...

  20. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...and/or perceived professional risk involved in setting up a CURE, especially if it’s a new approach within... amenable to in-class labs. Provost ultimately settled on a two-semester model, in which students learn...
Showing: 341 - 360 of 613 results