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We narrowed to 728 results for: STO;

Showing: 341 - 360 of 728 results

  3. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...sharing services, as well as #happydepositors who stopped by to say hello. Needless to say we all got very...the conference and talked to some scientists who stopped by the booth about amazing work already represented...

  8. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...interest for which you have several antibodies in stock. Why are there so many options for a single target...samples. If, however, you are performing an immunohistochemistry assay, you may want to rule out monoclonal...

  9. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...colony and do not subculture directly from glycerol stocks, agar stabs, or liquid cultures. Freshly transformed...plasmid yield that colonies streaked from a glycerol stock. Incubation for 12-16 hours tends to give higher...

  12. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...after subtracting out non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins...protein folding (tested using real science). Make a stock solution of 2.5 mM biotin (50X) because 5 mM is ...

  13. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...network. Which planet will you visit? Where will you stop for refreshment along the way? What ecosystem makes...developmental programs unfold, absorbing the stochasticity of molecular interactions and embedding individual...

  14. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...this sequence. The energy from this breakage is stored as a covalent bond between the cleaved 3′ DNA strand...are planning to transform right away OR you can store the reaction at -20C overnight. 4. Transform TOPO...

  15. Plasmid Cloning by PCR

    Type
    Blog Post
    ... want to clone from the start codon (ATG) to the stop codon (TGA, in this example). Assuming you are amplifying...taking the final 18 bases of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding...

  18. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...different cellular environments such as retina or glioblastoma). They also found that exo-AAVs were more resistant...brain tumor stromal cells in a mouse model of glioblastoma (Volak et al. 2018). The structure of exosome...

  20. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ... mechanism for this function is not yet fully understood, we can imagine that this transport is probably... possible, use low-bind or siliconized tubes to store samples as it is more difficult for viral particles...
Showing: 341 - 360 of 728 results