Skip to main content
Addgene
Showing: 341 - 360 of 711 results

  1. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...small molecules for use in the clinic and thereby restore motor neuron function in spinal muscular atrophy...mesothelioma sufferers. This disease is less widely understood and often misdiagnosed. Like other forms of mesothelioma...

  2. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...require a bachelor’s degree if you have a PhD. 4) Customize cover letters and even resumes The cover letter...project managment) your resume should also be customized to highlight different skills and to get the ...

  5. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...and Resources References Fellmann, C., et al., Cornerstones of CRISPR-Cas in drug development and therapy...CRISPR Guide CRISPR Plasmids and Resources CRISPR History and Development for Genome Engineering    ...

  7. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...the fundamentals. Our mission is to curate a one-stop reference guide for plasmids, so that you can spend...and references, and how to maintain your plasmid stocks. The guide also contains multiple protocols and...

  10. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...sharing services, as well as #happydepositors who stopped by to say hello. Needless to say we all got very...the conference and talked to some scientists who stopped by the booth about amazing work already represented...

  12. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...interest for which you have several antibodies in stock. Why are there so many options for a single target...samples. If, however, you are performing an immunohistochemistry assay, you may want to rule out monoclonal...

  15. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...this sequence. The energy from this breakage is stored as a covalent bond between the cleaved 3′ DNA strand...are planning to transform right away OR you can store the reaction at -20C overnight. 4. Transform TOPO...

  16. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...colony and do not subculture directly from glycerol stocks, agar stabs, or liquid cultures. Freshly transformed...plasmid yield that colonies streaked from a glycerol stock. Incubation for 12-16 hours tends to give higher...

  20. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...after subtracting out non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins...protein folding (tested using real science). Make a stock solution of 2.5 mM biotin (50X) because 5 mM is ...
Showing: 341 - 360 of 711 results