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We narrowed to 887 results for: TRI

Showing: 341 - 360 of 887 results

  1. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...antibodies against proteins embedded in membranes is tricky because they have fewer hydrophilic surfaces, require... randomized with a defined set of different trinucleotides (corresponding to different amino acids) in...exchange (FX) cloning technique which uses Type IIS restriction sites (Geertsma, 2013). Once the phagemids are...after a few washes, binding is detected using a colorimetric reaction. After the ELISA step, the narrowed-down...

  2. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... functional heterotrimers with endogenous Gβγ, enabling detection of activation triggered by endogenous... responsivity in screening ASAP3 variants. B) Electrical and optical recordings of spontaneous activity...sharing your experience with other researchers by contributing to the Addgene Antibody Data Hub.  Figure...

  3. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ... this way: "Addgene is one of the leaders in distributing plasmids amongst the academic community. On ...has partnered with the BioBricks Foundation to distribute the physical DNA samples, for when that's the...central dogma at the genome scale, it's going to be tricky to tame the rest of a cell. The BIOFAB plasmids...Who owns computer-programming languages? That's trickier. Sometimes it's the developers. But, it turns ...

  4. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...Vector  Percent of Neurons Transduced   Cortex Striatum Cerebellum AAV.PHP.B (parental) 49 +/- 7% 36...of a transgenic mouse. 2. Cell-type-specific restricted transgene expression in the CNS and PNS Previously...promoter or enhancer now allows for cell-type-restricted expression in both the CNS (figure 5 in Chan ...These capsids can serve as powerful cell-type-restricted gene vectors for neurons, no transgenic mouse...

  5. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...dilution series should be run in duplicate or triplicate. The closeness of the data points generated by... the standard dilution series in duplicate or triplicate. Replicates allows you to calculate the curve...within the linear portion of the graph. It does restrict the dynamic range of your standard curve, so if...and mix samples very well.  Use duplicates or triplicates to increase the accuracy of your experiment.  ...

  6. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...This post was contributed by Max W. Shen from MIT, Alvin Hsu Harvard University, and David R. Liu from...statistics provided by Google on 6/4/20). Many countries entered lockdown for weeks to months, pausing ...requests. We received plasmid requests from 56 countries, though the data is heavily dominated by the U.S...noise to each of the terms which make up our distribution, resulting in the following hierarchical model...

  7. DNA Purification Without a Kit

    Type
    Blog Post
    ...solution. Proteins and genomic DNA are removed by centrifugation. At this point, the plasmid DNA sample can ...binding DNA, the glass particles are pelleted by centrifugation and washed before DNA is eluted with water ...was free of agarose and could be digested with restriction enzymes.  Cellulose nucleic acid purification...phosphoric acid for 3 minutes followed by a short centrifugation for a total of four times before a wash with...

  8. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...decided this protocol would be a worthy first contribution to the protocols.io community. Did I mention... protocols, including making LB agar plates, restriction digest of plasmid DNA, and bacterial transformation... was created in 1999 and allows scientists to contribute protocols or provide links to protocols on their...subscription-based software, Labguru does offer free trials and pricing options for academic labs, departments...

  9. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...new AAV approach using regulatory elements that restrict expression to this cell type. To this end, they...the size of DNA fragments. Depending on which restriction enzyme is used to cut the plasmids encoding the...are shared through Addgene without licensing restrictions. Use these instructions to prepare the PSU ... of telomere length regulation called telomere trimming and have proposed a role for TZAP, a Kruppel-like...regulating the upper-bound of telomere length. Telomere trimming can be assayed by looking for the production of...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...single crRNA The Feng Zhang lab used Cas13a from Leptotrichia shahii (LshCas13a) to begin to characterize ...et al., 2017). They characterized Cas13a from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion...global RNA expression, cell stress, and RNA size distribution. When comparing LwaCas13a to shRNA, they found...community. Note: Cary Valley and Mary Gearing contributed to the update of this post. References 1. Abudayyeh...

  11. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...This post was contributed by the Mesothelioma Cancer Alliance. We have learned much about the causes ...techniques are currently being tested in clinical trials to detect mesothelioma earlier than ever. From ...been banned in the United States and many other countries, and it is still imported, exported, and used ...from this dangerous material. This post was contributed by the Mesothelioma Cancer Alliance. Additional...

  12. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...made great strides to map brain regions and allocate specific functions to them. Electrical recordings...circuit by integrating neuronal signalling and triggering release of the stress hormone norepinephrine ...levels in general, or anxiety associated with psychiatric diseases such as schizophrenia or bipolar disorder...

  13. Hot Plasmids - November 2023

    Type
    Blog Post
    ...protein ticker tape Teaching an old antibody new tricks   New compact, efficient prime editors By Susanna....10.13.463862.    Teaching an old antibody new tricks By Ashley Waldron While IHC protocols typically...thick-section and whole-mount labeling techniques. The Trimmer lab’s anti-PSD-95 [K28/43R] recently passed their...antibodies in Addgene’s collection since we began distribution and has been verified to perform well in standard...

  14. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...or G) is supplied as a dideoxyribonucleoside triphosphate (ddNTP) so that after its addition, DNA synthesis...attached to the flow cell membrane and have an electrical current running through them. DNA or RNA is pulled...each nucleotide passes through the nanopore, the electrical current recorded by the instrument changes. Base-calling...written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly...

  15. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...envelope proteins from another virus to either restrict or broaden the host cell range (tropism). Pseudotyping...vesicular stomatitis virus G protein. VSV-G is a trimeric protein that binds phosphatidylserine and low-...preparation, VSV-G is stable and can withstand ultracentrifugation which helps scientists achieve a high titer..., Young JAT, Callaway EM (2007) Monosynaptic Restriction of Transsynaptic Tracing from Single, Genetically...

  16. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...and last updated Aug 12, 2021. This post was contributed by guest bloggers, Yiwen Zhang, a Ph.D. candidate... pTargetF with the ccdB gene containing BsaI restriction enzyme sites at its ends to generate pEcgRNA....that is 2.6 times higher when compared to the industrial strain. In our metabolic engineering case study...high-yielding strain which had 6 times the yield of the industrial strain. Not only is MUCICAT better, it is faster...

  17. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...pair of restriction enzymes. These enzymes, Nt.BbvCI and Nb.BbvCI, recognize the same restriction site, ... be sense. Does the orientation of the BbcVI restrict site matter? Most of the time no, but there are...directions, you just need to use a plasmid with BbvCI restriction sites in the same orientation as the gene or ...

  18. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...type and laser voltage. The voltage describes an electric potential that can be applied to the photomultiplier...photomultiplier inside the cytometer to increase the electric current and thereby the signal strength. So, we...dimensions displayed in the plot. (B) A more restrictive gate results in fewer cells being considered ... personal preference, some people like to make stricter gates while some like to make looser ones (Figure...

  19. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...development, and the shaping of the extracellular matrix. During immune responses, this same conformation...resulting in cells that elongated and morphed asymmetrically (Jo et al., 2022). By contrast, LVDP-binding...conferred less force, leading cells to spread into symmetrical circles. While elucidating the role of tension...Addgene to accelerate biomedical research by distributing antibodies developed by IPI. Our integrin antibodies...

  20. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...is far from perfect. One of the most limiting attributes of rats is their difficult to manipulate genome...making them useful for in vivo studies. These attributes make frog eggs some of the most useful tools ...utilization, Arabidopsis has made significant contributions to understanding plant and crop science alike...). E. coli as a Model Organism. News-Medical. Retrieved on September 18, 2020 from https://www.news-medical.net...
Showing: 341 - 360 of 887 results