...cloning rings or following labor-intensive limiting dilution protocols? If this sounds like you, then flow ...control, the higher the level of expression. A routine flow cytometry experiment uses 2 or 3 different...you would need a way to determine the relative contribution of yellow and orange from FITC and that from...

...carried over from the precipitation stage, and then eluting the DNA from the substrate. In a column-based approach... magnets to isolate the beads for the wash and elution steps.  Both bead- and column-based DNA purification...is a large consideration for most labs. Many institutions have preferred vendors which may reduce costs...

...co-founder and CSO Melina Fan's words of welcome! Executive Director Chonnettia Jones and Board Member Joanna...main purpose of the party was to celebrate the contributions of each and every Addgenie, from our co-ops ...

...present our own data on plasmid deposits and distirbution for these fast moving fields.  Addgene is a ...with data comparing Addgene global deposit and distribution trends for Zinc Finger, TALE, CRISPR, and SynBio...

... and Olsson, 1971), plant lectins (wheat germ agglutinin WGA, Schwab et al., 1978) or fluorescent molecules...). Efficient retrograde neuronal transduction utilizing self-complementary AAV1. Mol Ther 16, 296–301....Javoy-Agid F., and Agid Y. (1978). Labeled wheat germ agglutinin (WGA) as a new, highly sensitive retrograde tracer...

...contains 139,868 reagents from 5,821 labs in 1,249 institutions across 55 countries.  Our first request was ...2,002,539 materials to 254,034 scientists in 8,900 institutions across 111 countries. Just eight days into 2024...

...genus members to the specified international institution. Requesters will need to provide a brief description... Addgene will then apply for the license for distribution to the requester’s organization. A non-refundable...

...Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland. After completing...department at MIT. Crystal now works on protein therapeutics at Novartis - listen to learn more about Crystal...

...high risk by these screens can then undergo more routine screening allowing for early cervical cancer detection... our Cancer Pages Learn about Global Plasmid Distribution ...

...now with more than 50% international plasmid distribution!) Chapter 8 - Opportunities to create repositories...Genetics Institute in Cambridge, MA and Sequana Therapeutics in San Diego, CA. She earned a B.S in Biology...

...suggestions for defining the problem and for finding solutions. Even better than just reading this article - ...by working together on the event planning and execution. I am seeing many similar examples of science ...

...scientific conference? As a member of the DayCon Executive Board, I was fortunate to see the event planning...SITN’s website. 65 audience members gave up a beautiful summer Saturday to learn about science, but they...

...eight modular repeats, each of which binds a consecutive base in its RNA target. By changing amino acids...endless.” Abil says the new tool might even find therapeutic application in addition to their use in basic...

...Maintaining Institutional Knolwedge and Continuity - SITN's key technique for retaining institutional knowledge...

...are affected. As of now, phage therapy is not routinely done in most parts of the world. In the US, phage... only used phage in basic research but not therapeutically: not all labs in Phage Directory have the resources...

...CHimeric IMmune Editing system) to enable the constitutive, ubiquitous knockout of single genes in immune...developed X-CHIME, a suite of four systems which utilizes custom mouse strains and lentiviral expression...

...2014! Get excited, it’s a new year. A time for resolutions: to wake up and get to the lab earlier, to read... notebooks (ELNs) have gained traction with pharmaceutical companies, academic labs have been slower to...

...feel connected.    As I became accustomed to the routine, I realized that the “9-5” was not as scary as ...completing their co-op at Addgene. This is her second contribution to the Addgene blog.      More resources on...

...driven project. Both awardees emphasized the contribution of students in their labs and the importance...History of CRISPR Cas - A tale of survival and evolution A Powerful New Way to Edit DNA (by Andrew Pollack...

...75,000 plasmids to more than 1,500 different institutions in Europe. In the last four years alone, the...fantastic numbers, a lot of European scientists and institutions are not aware of what Addgene can do for them...