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We narrowed to 743 results for: UTY;

Showing: 341 - 360 of 743 results

  1. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...play significantly into the realm of clinical therapeutic development as CRISPR is used not only to study... Dillard, and Ayokunmi Ajetunmobi for their contributions to this work. Søren Hough is a Biochemistry ...PMC4320661. 14. Crosetto, Nicola, et al. "Nucleotide-resolution DNA double-strand break mapping by next-generation...

  2. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...cloning rings or following labor-intensive limiting dilution protocols? If this sounds like you, then flow ...control, the higher the level of expression. A routine flow cytometry experiment uses 2 or 3 different...you would need a way to determine the relative contribution of yellow and orange from FITC and that from...

  3. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...carried over from the precipitation stage, and then eluting the DNA from the substrate. In a column-based approach... magnets to isolate the beads for the wash and elution steps.  Both bead- and column-based DNA purification...is a large consideration for most labs. Many institutions have preferred vendors which may reduce costs...

  4. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...lab recommends using the Epicentre QuickExtract solution to perform this step to extract genomic DNA from... Zhang lab uses around 50 µl of the extraction solution, runs the protocol, then uses around 2–4 µl in...Mukherjee, K., & Carroll, D. (2013). Donor DNA Utilization During Gene Targeting with Zinc-Finger Nucleases...

  5. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...co-founder and CSO Melina Fan's words of welcome! Executive Director Chonnettia Jones and Board Member Joanna...main purpose of the party was to celebrate the contributions of each and every Addgenie, from our co-ops ...

  6. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    ...present our own data on plasmid deposits and distirbution for these fast moving fields.  Addgene is a ...with data comparing Addgene global deposit and distribution trends for Zinc Finger, TALE, CRISPR, and SynBio...

  7. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... and Olsson, 1971), plant lectins (wheat germ agglutinin WGA, Schwab et al., 1978) or fluorescent molecules...). Efficient retrograde neuronal transduction utilizing self-complementary AAV1. Mol Ther 16, 296–301....Javoy-Agid F., and Agid Y. (1978). Labeled wheat germ agglutinin (WGA) as a new, highly sensitive retrograde tracer...

  8. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...contains 139,868 reagents from 5,821 labs in 1,249 institutions across 55 countries.  Our first request was ...2,002,539 materials to 254,034 scientists in 8,900 institutions across 111 countries. Just eight days into 2024...

  9. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...genus members to the specified international institution. Requesters will need to provide a brief description... Addgene will then apply for the license for distribution to the requester’s organization. A non-refundable...

  11. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...now with more than 50% international plasmid distribution!) Chapter 8 - Opportunities to create repositories...Genetics Institute in Cambridge, MA and Sequana Therapeutics in San Diego, CA. She earned a B.S in Biology...

  13. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...suggestions for defining the problem and for finding solutions. Even better than just reading this article - ...by working together on the event planning and execution. I am seeing many similar examples of science ...

  14. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...scientific conference? As a member of the DayCon Executive Board, I was fortunate to see the event planning...SITN’s website. 65 audience members gave up a beautiful summer Saturday to learn about science, but they...

  15. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...eight modular repeats, each of which binds a consecutive base in its RNA target. By changing amino acids...endless.” Abil says the new tool might even find therapeutic application in addition to their use in basic...

  19. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...2014! Get excited, it’s a new year. A time for resolutions: to wake up and get to the lab earlier, to read... notebooks (ELNs) have gained traction with pharmaceutical companies, academic labs have been slower to...

  20. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...feel connected.    As I became accustomed to the routine, I realized that the “9-5” was not as scary as ...completing their co-op at Addgene. This is her second contribution to the Addgene blog.      More resources on...
Showing: 341 - 360 of 743 results