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We narrowed to 770 results for: VARS

Showing: 341 - 360 of 770 results

  2. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...originally described by Theodor Förster in 1948 as a variation of the more commonly observed light emission by...several excellent reviews have collated data from various papers to make the decision easier. 1. Müller, ... because the ratio of acceptor to donor fusions varies with transfection efficiency and any unpaired fluorescent...

  3. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... and some, such as varenicline, are already approved for clinical use. Varenicline has been further modified...promoters, AAV serotypes, and the ever-growing variety of recombinase systems. Picking Ligands Picking...

  4. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...protocol [3] provides, in exquisite detail, the various steps required to produce your fiber optic implant...materials and methods of a paper that uses the variant you wish to use to determine the ideal excitation... be controlled. As discussed above, each opsin variant has slightly different activation/inactivation ...

  5. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...movement and is part of a bigger movement in a variety of research sectors to provide open source technologies...engineers, and biologists working together on a variety of projects including the OpenWorm project. The...model, and the biologists can get involved a in variety of aspects from the molecular biology to learning...

  6. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...because different CAPTURE cell lines will have varying levels of dCas9 and gRNA expression. Additionally...CRISPR users Learn how to Choose the Best Cas9 Variant for Your Next Experiment Learn More About Designing...Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic Regions of Interest Here...

  7. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ... 2025). The team characterized and deposited a variety of constructs for visualizing cellular components...-based biosensor for cAMP dynamics. The mGold2 variants significantly enhanced photostability — 25-fold...with BioRender.com. When delivered by AAV to various brain regions in mice, ATLAS mediated anterograde...

  8. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...The VSD components have historically come from a variety of sources – voltage-gated potassium channels found...Chemigenetic Sensors These types of sensors can have a variety of different components, but all contain a FRET...upon host infection. Packaging viruses also have various serotypes – the surface proteins that confer affinity...

  9. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...versatility of Cas effectors allows for a wide variety of screens, including interference, activation,...using VSV-G. In contrast, AAV can be packaged in a variety of capsids. To date, at least 12 different capsids...the amount of cloning, another option is to use variants of Cas9, like split Cas9, or a smaller Cas9 ortholog...

  10. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...the level of autofluorescence across various tissue types varies, it is critical to include an unstained... to avoid unmixing issues from batch-to-batch variation. Tandem dyes (two fluorescent molecules conjugated...

  11. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...biology in our Plasmids 101 eBook! Researchers use various techniques to control protein expression for experimental...protein tags and separated from contaminants using a variety of purification methods. Deciding which expression...expression of recombinant proteins. Many cells from various types of plants such as maize, tobacco, rice, sugarcane...

  12. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...volume = more cells = more plasmid. Plasmids can vary in copy number, the number of plasmid copies supported...plasmid DNA yield. Different plasmids and strains will vary in their optimal growth conditions. For many high...cell density or by harvesting and miniprepping at various time points. Measuring the optical density of the...

  13. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...discovered that there are many ways to get a taste of a variety of careers while still working towards your degree...graduate office arranged opportunities to meet with various professionals, and there were multiple avenues ... Find Plasmids for Your Research Learn About A Variety of Different Fields on Our Collections Pages ...

  14. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...Technology Development Fellow at the Wyss Institute and Harvard Medical School. Scientists making transgenic organisms...seeks to keep the organisms in the laboratory. It varies by organism, but your local biosafety officer should... cas9 gene). An immunizing reversal drive is a variant that also spreads through the wild population and...

  15. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...PspCas13b-ADAR2DD(E488Q) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated...targeted 34 pathogenic G->A mutations from the ClinVar database. They successfully edited 33/34 sites ...in therapeutic applications, the authors tested various dRanCas13b truncations that would allow packaging...

  16. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ... new cutting edge science, researchers of all varieties must stay current with literature and emerging...clear that while several people across teams had various levels of experience with bioinformatics and HTS...welcome to ask any questions they have.  These variations are suited for different applications and should...

  17. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...scientists understand how our vectors perform in various experimental settings. It is a curated set of technical...the knowledge base of AAV vector performance in various brain regions and cell types. Power in numbers ...Boulis, N. M., & Donsante, A. (2019). Lot-to-Lot Variation in Adeno-Associated Virus Serotype 9 (AAV9) Preparations...

  18. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...one used in Luxturna™, can efficiently transduce various types of retinal neurons. Nowadays a toolbox of...serotypes are currently used in clinical trials, a variety of engineered ones have shown great promise in ...improve the technology: engineering of new capsid variants, designing expression cassettes to regulate and...

  19. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...infection and screening by PCR enrich for capsid variants with increased ability to infect the cell type...transduced with multiple viral particles and labeled a variety of colors (figure 4b). But notice in figure 4b ... BP, et al. Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain...

  20. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...10% of DNA repair, with this rate varying across cell types. Variance can be caused by cell cycle rates...mutated in cancer cells, specifically in breast and ovarian lines commonly used in lab. It is recommended you...
Showing: 341 - 360 of 770 results