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We narrowed to 573 results for: Vit

Showing: 341 - 360 of 573 results

  1. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...vector that enables protein expression in any in vitro translation system. The gene set was carefully chosen...been used to produced recombinant proteins for in-vitro assays: Koch, Daniel, et al. "A pull-down procedure...

  2. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...Read on to learn how CRISPR can take on HIV-1 in vitro, and what obstacles must be overcome for clinical...off-target cleavage. In both cases, translating the in vitro work into an animal model comes with two key hurdles...

  3. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...from pLac repressed more   LacZ β-galactosidase activity abolished   lacY Lactose permease inactivated...increases plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability, reduced...

  4. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...promoter upstream of the left homology arm, perform in vitro transcription, and use the IVT product as a template... design has been shown to increase T7 promoter activity. To avoid re-cutting by Cas9 after editing, make...

  5. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous protein tagging as it is only 19...No photoexcitation High Ion specificity High sensitivity Easy expression Gives ratiometric data The result...

  6. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...problems for gene therapy applications. Cross-reactivity of AAV serotypes varies by species and route ....2008.123 Dhungel, B. P., Xu, H., Nagarajah, R., Vitale, J., Wong, A. C. H., Gokal, D., Feng, Y., Tabar...

  7. A Control for All Seasons

    Type
    Blog Post
    ...specificity control, label control, and cross-reactivity control when multiplexing. A, wild-type and knockout... included to uncover secondary antibody cross-reactivity. Non-specific binding  Primary antibody specificity...

  8. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...genome to be probed for proteins with host-binding activity without the need for genome sequencing or annotation...nitrocellulose for subsequent probing for cell-binding activity.  First, cut out 20 Petri dish-sized circles of...

  10. Finding Your Science Policy Path

    Type
    Blog Post
    ...Plus, just one role can encompass many of these activities leading to a rich day-to-day work life.  Action...professional societies, and industry through convening activities, formal lobbying, or strategy setting.  The “...

  11. Antibodies 101: Validation

    Type
    Blog Post
    ...core goals of antibody validation:   Confirm reactivity with the target antigen - Recognition of an immunogen...methods. These approaches have relatively low sensitivity, which can be problematic if you are trying to...

  12. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...have allowed for real-time monitoring of neuronal activity across substantial 3D volumes. In addition to ...Weijian, and Rafael Yuste. In vivo imaging of neural activity. Nature Methods 14.4 (2017): 349-359. PubMed PMID...

  15. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...DNA-Free Methods RNA Delivery: For this approach, in vitro transcribed RNAs are serially transfected using ...functional tests. Cells can be differentiated in vitro to form embryoid bodies which are compact balls ...

  16. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...to expose them. You’ll need to make sure the sensitivity of your exposure solution matches the expected...Incubation and Stacked Film Exposure Improve Sensitivity in Western Blotting. Journal of Pharmacological...

  19. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...decorating your lab for all upcoming wintertime festivities: Hanukkah, Christmas, ugly sweaters, Flannel ...
Showing: 341 - 360 of 573 results