... genes in mammalian cells (Bressan et al. 2017; Liang et al. 2017). Current methods of epitope tagging...Characterize clonal lines by ICC and PCR genotyping (Sanger sequencing); use primers flanking the tag sequence...References 1. Bressan RB, Dewari PS, Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant ...Rev Genet 32: 601-618. PubMed PMID: 9928493. 4. Liang X, Potter J, Kumar S, Ravinder N, Chesnut JD. 2017...

... constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini (6), and Chari/Mali... 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries for...: 27760321. PubMed Central PMCID: PMC5081405. 6. Wang, Tim, et al. "Gene essentiality profiling reveals...

...Although white light is entering your hand, only orange/red light can be seen exiting through the tissue... Light is projected through the coverslip at an angle such that when it reaches the interface between ...when acquiring multiple images over a large axial range. 2-photon imaging, which restricts excitation and...confocal microscopy, 3rd edn. Springer (2006). 7. Yang, Weijian, and Rafael Yuste. In vivo imaging of neural...

... is working for you, then there is no reason to change it. But what if a kit isn’t working for you, or...kits are designed to accommodate specific volume ranges of culture as their input (Table 1). When you feel...addition to standard purification kits, there’s a range of specialty kits available for plasmid purification...you do want to make sure you’re getting the best bang for your buck. If you’re unsure if a kit is a good...

...by 草方 (@gracie_fang) That’s right, researchers of the world. Cap your Eppendorf tubes, hang up your ...at like 23:00, so I decided to make a giant snow angel for biologists in our building. Unfortunately, snow...

...the make-up and scope of your graduate projects changed throughout grad school? 22:05 - 26:10 : What's ... studies developmental gene regulation with Dr. Angela DePace in the Department of Systems Biology, and... genetics at the University of California, Los Angeles. She enjoys chromosome biology and reading Ben ...

...Host-parasite Interactions, Institut Pasteur, Paris) that changed genome editing in the malaria community for the...it all settles down and the value of each item changes from what the reviewers so painstakingly assessed...offline tools requiring expertise in one programming language or another. Supported organisms: This section...Top tool for first timers: You need to learn the language and what the parameters represent. The best learn-by-doing...

...lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute and was designed and...take a look at the protocol kindly provided by the Zhang lab. Even though lentiCRISPR v2 has only been available... 2. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries for...

..., in the labs of Chase L. Beisel and Rodolphe Barrangou, had applied the CRISPR/Cas9 system to developing... author F. Ann Ran, a graduate student in Feng Zhang's lab, in a press release. "In the future, it could...Powerful New Way to Edit DNA" and "This Protein Could Change Biotech Forever."  David S. Weiss of Emory University...

...find oneself unsure of the next steps. Harvard PI Angela DePace has put personal development at the center... is to give honest, constructive feedback, set tangible research and career goals, and celebrate accomplishments...getting used to, yearly planning meetings have tangible benefits for both PI and trainee. DePace and her...

...pile of lab notebooks and binder on your desk. Arrange them in chronological order (or by project, if ...lists of your upcoming tasks, set deadlines, and arrange automatic email reminders. These programs can be...necessary time and resource investment required to change the status quo. But with recent improvements to...

...States - Cambridge, MA to be exact. Today, that changes. Today, Addgene launches a brand new Europe office...specialising in Molecular Biology. She has worked in a range of labs - cancer research, microbiology, virology...scientists together from all over the world to exchange research materials in an easy way. She has a passion...

...wanted to major in biochemistry. There, I had exchanged contact with Amy C., Addgene’s intern coordinator...into perspective just how much one’s environment changes them. Addgene gave me the chance to become a real...opened its arms to me from day one. Addgene has changed my life for the better, and I wouldn’t want it ...

... of disciplines. Cast your net wider, explore tangential subjects, and let curiosity guide your intellectual...beyond your immediate field, fostering a diverse range of perspectives and sparking innovative thinking...embraces the unexpected and encourages exploration of tangents or seemingly unrelated ideas. It's beneficial ...

... origin of replication (which controls the host range and copy number of the plasmid) and typically include...biologists to broadly utilize plasmids across a wide range of applications. Some common plasmid types include...Produce enzymes that will make specific, controlled changes to an organism’s genome (genome engineering) Produce...

...cytosine nucleotides, we might need to send it for Sanger sequencing to verify the sequence within that region... region. We have designed many primers for Sanger sequencing at Addgene. By using Snapgene’s “Import primers... difficult regions and send the plasmid off for Sanger sequencing.     If a repetitive region is present...

...). G. stearothermophilus grows at temperatures ranging from 30-74 °C, with an optimal growth temperature...or G) Spacer Length 20 nt 22 nt Temperature Range for Optimal Activity 35 - 45 °C 50 - 70 °C Genome...were incubated in reaction buffer at temperatures ranging from 25-80 °C. Activity is expressed as Kcleave...

...products Dr. Idah Sithole-Niang, University of Zimbabwe, Zimbabwe Dr. Idah Sithole-Niang (above, left) is an... diverse groups of scientists working in a wide range of research fields and geographical locations. We...

...conflicts are a common reason for scientists to change labs – do as much as you can to ensure a good fit...possible. Attend a lab meeting. Spend some time hanging out with members of the lab. It is a warning sign...advisors and other related resources. Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...

...region may be changed.   Chimeric antibodies can help A chimeric antibody is made by changing an antibody...will react with the same secondary. If you were to change one of the antibody’s isotypes, however, you could...