We narrowed to 393 results for: prep
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TypeBlog Post...Forster, A. C., Skingle, D. C., & Symons, R. H. “Preparation and uses of photobiotin.” Methods in Enzymology...
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New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...7. https://doi.org/10.1038/s41557-023-01214-0. Preprint on bioRxiv: https://doi.org/10.1101/2022.09.26.509516... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog Post...fluorescence visualizer with 9V battery Approach :This preprint uses a previously developed method to detect SARS-CoV... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post...community is the ultimate value proposition. By preparing a budget before hand, you’re saving the institute... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post... and here I summarize what I learned both in preparation for my talk and at the minisymposium. You can... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...confused. It was supposed to be hard. I was mentally prepared to go through a learning curve that would result... -
Finding Your Perfect Job After University
TypeBlog Post...lacking during degree courses; they never really prepare you for the amount of failure and problem solving... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post... (SDS-PAGE) is the first step of a western. To prepare the samples for SDS-PAGE, measure the protein content... -
A Control for All Seasons
TypeBlog Post...antibody binding. For this control, samples are prepared following the standard protocol, but the primary... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...writing fellowships, giving oral presentations, and preparing for job interviews, expanded profesional networks... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...Identity of Recombinant Adeno-Associated Viral Preparations from Viral DNA Extracts. Human Gene Therapy ... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post... but it’s a very good practice for prevention. Prepare a small quantity of media stock that would be used... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...your work. Immediate steps that you can take to prepare for the green card process Update your CV and ... -
Antibody Guide
TypeGuide...conjugating them in the lab? How will the sample be prepped, and will this or other factors affect epitope ...isotype. Overview of application Fix your samples. Prepare your samples using permeabilization, antigen retrieval...and controls follow that of IF (above). Sample preparation, signaling excitation, and assay readout vary... -
15 Hot Plasmids from 2017
TypeBlog Post...licensing restrictions. Use these instructions to prepare the PSU ladders! Rosado, et al. Autophagy. 2008... -
Technical Design of a Western Blot
TypeBlog Post...binding to your protein of interest. Protein preparation Before you can blot your proteins, you’ll need... -
AAV Production in HEK293 Cells
TypeProtocol... °C for 45 min. You are now ready to purify your prep . The clarified supernatant can be kept overnight...mM Tris HCl, 150 mM NaCl, 2 mM MgCl2) Reagent Preparation DMEM Complete : 10% v/v FBS and 4 mM L-alanyl-L-glutamine...et al., 2018 (Link opens in a new window) ). To prepare D1 + 0.1 M sorbitol, add 57 mL of 1.46 M sorbitol... -
AAVED
TypeCollection...Melina Fan/Leila Haery 5:00 PM Adjourn Meeting Preparation For Speakers: Each speaker will give a 20 minute... reagents)? For All Attendees: To help us best prepare and compile resources for this meeting's publication...commitment to open science! Please see the meeting preparation section for how to best help contribute to this... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...using traditional cloning strategies may seem preposterous. Luckily, the Pierre Neveu laboratory designed... -
Immunology Research Plasmids and Resources
TypeCollection...LGR1, MGC141667, MGC141668, ODG1 GAL galanin prepropeptide GALN, GLNN, GMAP, MGC40167 GALP galanin-like...GHRFR, GHRHRpsv, GRFR GHRL ghrelin/obestatin prepropeptide MTLRP, obestatin GHSR growth hormone secretagogue...PGRN GRP gastrin-releasing peptide BN, GRP-10, preproGRP, proGRP GUCA2A guanylate cyclase activator 2A ... OSMRB OSTN osteocrin MUSCLIN OXT oxytocin, prepropeptide MGC126890, MGC126892, OT, OT-NPI OXTR oxytocin...pro-melanin-concentrating hormone MCH PNOC prepronociceptin PPNOC POMC proopiomelanocortin ACTH, CLIP,...