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...measurement. Hit the tare button (or zero button) to set the balance to zero. Pro-Tip If you’re weighing a...

...Pathway Clone Collection 2.0. The collection is a set of vectors for use with the Gateway Cloning Platform...

...there include: Google Groups - I've specifically set up a forum where NgAgo users can share protocols ...

...allow for two sets of microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette into the locus...protocol for MMEJ-mediated knock-in of a GFP-Puro cassette into a given locus, just upstream of a stop codon...microhomology to the insertion locus flanking the GFP-Puro cassette. Three double stranded breaks are necessary for...for knock-in: one on either side of the GFP-Puro cassette and one in between the 5’ and 3’ microhomologies...of PITCh. The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and 3' microhomology and PITCh-gRNA...creates two DSBs in the PITCh plasmid to release the cassette, and an insertion-locus-specific gRNA cleaves ...and 3’ microhomologies are added to the GFP-Puro cassette via PCR, and this construct is inserted into the...

...future in under 200 pages is an impressive task to set oneself. As a scientist who enjoys learning a little...

...have the idea that if you don’t get your PhD you’re set up for failure. Listening to Kenkel’s story has helped...

...convex scaffold and are randomized with a defined set of different trinucleotides (corresponding to different...

... notice for the first meeting so that people can set aside time in their schedules and read the paper ...). This notice can be shortened as the meeting settles in and people get the time recurrently carved out...

...chromoproteins are actually a subset of the fluorescent protein family. What sets them apart is the way they...

...a large number of hits to one sequence/gene - we set our threshold at >100 hits - immediately raises a...

...containing serum in each well. A 200 ul pipettor is set to 200 ul, wiped down with 70% ethanol, and a box...

... you identify bacterial and yeast contaminants. Set up a routine to split cells Don’t be lazy about splitting...

...list of posters of interest, then you’ve already set yourself up for success. But sometimes, attendees...

... the same target. In the 1980s, Munro and Pelham set out to address this issue by developing generic strategies...

... brightness is compared to that of EGFP which is set as 1. Some proteins are very dim (e.g. TagRFP657,...

...discovered in 1856 by William Perkin. His discovery set off a flurry of activity and the majority of the ...

.... suggest that it could be valuable in medical settings where antibiotic resistant bacteria have previously...Even when researchers develop new antibiotics, the onset of resistance is swift, as few as five years after...

...be useful for your studies.  Find the plasmids to set up your screening systems at Addgene! Schematic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...

...sales-y”. The work includes reviewing customer data sets, troubleshooting protocols, analyzing information...

... the use of a given timer in many experimental settings. Monomeric fluorescent protein timers Since the... simplifies cloning and makes your experimental setup less cumbersome. 3. Portability across multiple ...