Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,010 results for: RING;

Showing: 181 - 190 of 1010 results

  1. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...to help students during an interview. When they were instructed to use the three-ring binder as a starting...transferable skills learned while working in the lab and offering advice on areas where you might need some help...creativity is absolutely a transferable skill developed during PhD training. Creativity is required to develop...Whether it’s finding the source of contamination or figuring out why the lab is so cold, we’ve all had to think... need ahead of time and mute your notifications during. (Boyes, 2019) If you’ve taken the time to do this... assure that all participants feel comfortable sharing their ideas? And how do we give people the opportunity... The purpose of this form was to encourage the sharing of ideas, not to give people more work. Identify...

  2. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...camera lens without having to physically rotate the rings around the lens. It allowed me to focus on the subject...constraints given. And there were many constraints during the COVID-19 pandemic. The Pandemic Pivot Back...Even Blugene is practicing social distancing and wearing their mask. But while the camera is here, the operator...chair not watching their actors live on set, but peering at a 7-inch wireless monitor. Wireless video technology...control, and extra hand sanitizer.    Cycle 4: Venturing into the Lab Design: The lab is the beating heart...heart of Addgene. It’s why we exist, storing plasmids, and distributing them to labs that need them. The...projects lined up, some that started production during the middle of lockdown, and some that will spin...

  3. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...spent hours in the biosafety cabinet with cloning rings or following labor-intensive limiting dilution protocols...is used for a variety of applications such as measuring target protein expression levels, assessing post-translational...uses 2 or 3 different fluorescent colors each measuring a different target. Flow cytometers however are...methanol, fix and permeabilize in a single step by denaturing proteins and dissolving lipids including those...specialized components that can divert a subset of cells. During FACS, the sample stream oscillates to generate ...be targeted with specific fluorescent antibodies during the flow cytometry experiment and will appear as...of yellow and orange from FITC and that from PE. During compensation the signal arising from a specific...

  4. Pouring LB Agar Plates

    Type
    Protocol
    ... Protocols Pouring LB Agar Plates Pouring LB Agar Plates You may also like......solidifies in the bottle while you’re pouring, you should stop pouring and re-make the gel-mix. If you’re... autoclave tape. The autoclave tape will darken during the autoclave process if your sample has spent ...in the autoclave, you should prepare your plate pouring station: Find an empty section of lab bench with... the flame just to the side of where you’ll be pouring your plates - be sure to leave room for your bottle...appropriate antibiotics, and a section for active pouring. Prepare your antibiotics. Prior to adding your...1000x stock solution. For example: If you’ll be preparing plates with a final concentration of 100 ug/mL...

  5. Fluorescence Titering Assay

    Type
    Protocol
    ... Protocols Fluorescence Titering Assay Fluorescence Titering Assay for Lentivirus You may also...cells and debris. Lentiviral titer can decrease during cycles of freeze-thaw. If you are freezing and ...consider wells with less than 40% fluorescent cells. Titering methods assume one integration event per cell....

  6. Colony Formation Titering Assay

    Type
    Protocol
    ... Protocols Colony Formation Titering Assay Colony Formation Titering Assay for Lentivirus You may also...luer-lock filter, Corning 431229 10 mL luer-lock syringe, BD BD300912 0.45 μm polyethersulfone filter, Nalgene...added at the time of viral transduction, and not during the cell seeding step. Procedure Before beginning...cells and debris. Lentiviral titer can decrease during cycles of freeze-thaw. If you are freezing and ...

  7. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...like to go to. During the conference Take notes during talks. If you have questions during the talk, jot...conference depends on what you put into it before, during, and after the meeting. Let’s break it down into...Try to avoid jargon in this initial introduction. During the conference When you meet someone new at a conference... new method that you learned about at the talk, bring it to your next lab meeting. Don’t be afraid to ...for both instances. If your poster material is nearing publication, consider uploading your work to a ...poster) so your visitors can have a DOI to go to. During the conference Arrive at the poster session early...the board and meet your poster session neighbors. During the session, if someone comes up to my poster, ...

  8. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... from Meiring et al., 2022, under CC-BY 4.0 license. A naturally occurring microtubule-severing protein...cells with physical structure, separate chromosomes during mitosis, support transport of organelles and vesicles...localized microtubule disassembly, Opto-katanin (Meiring et al., 2022), that overcomes many of those limitations...Opto-katanin induces fast, localized microtubule severing. A) Schematic of Opto-katanin constructs and activity...highly localized, rapid and reversible microtubule severing and has very low dark-state activity and toxicity...can be critical when, say, the gene is essential during development and a knockout is not feasible or when... One great way to do this is to use CRY2-CIB1 clustering. Fuse either CRY2 or CIB1 to your protein of ...

  9. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...This year, the IMP mentoring program extends its scope to the fields of Engineering, Economics, and Philology...internationalization and there is a lack of expert mentorship during undergraduate and graduate studies. Students also...Mentor Circle Programs at BWH and providing career mentoring to several students from Spain and all over the...in several countries, however programs like IMP, bring closer than ever (although not physically) the ideas...exist outside their immediate frontiers. Moreover, sharing a culture/country of origin with an international...2014-15, University of Valencia): “It is very inspiring to be in contact with mentors who are at a professional...the ability to accept feedback and adapt their mentoring styles to the mentee’s personality and professional...

  10. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ..., try lowering the voltage and using a longer run time.   Samples can run into empty neighboring lanes... two most common (non-technical) issues with transferring are smaller proteins going completely through...through the membrane or larger proteins not transferring completely onto the membrane. If you’re not sure ...sure if your protein of interest is over-transferring (going completely through your membrane to the other...other side), you can check by using two membranes during your transfer instead of one. If you see signal...both membranes, you know your protein is over-transferring! If you see very little signal, but you know... sample of a large protein, you may be under-transferring.  Membrane pore size Membranes come in different...
Showing: 181 - 190 of 1010 results