We narrowed to 532 results for: abo.3

... near-infrared photosensitizer. Nature Methods,13(3), 263-268. doi:10.1038/nmeth.3735. PubMed PMID: 26808669...PMID: 18706812. PubMed Central PMCID: PMC4445427. 3. Bulina, M. E., Chudakov, D. M., Britanova, O. V.,... in culture and in vivo.  Read on to learn more about this killer illumination technique! What is a photosensitizer...within the near-infrared (NIR) spectrum (>620 nm). Above is a brief schematic of how the MG-2I-dL5** FAP-...use MG-2I-dL5** with your research? Let us know about your experiences using photosensitizers in the comments... to Receive the Fluorescent Protein eBook Learn about Small Molecule Fluorophores Resources on Addgene.org...

...MS2 RNA aptamers at the tetraloop and stem-loop 2; 3. The MS2-P65-HSF1 plasmid which expresses the activation...LS, Kampmann M, Weissman JS. Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. Epub 2014...that screen with a system you are really confident about working for most genes and individual targeting ... system, Konermann explained, was new knowledge about Cas9’s crystal structure. “I thought maybe we could...RNA we use. What we have to do now is learn more about the rules.” He said that there is no theoretical...References & Further Reading: For more information about SAM, including a tool for getting optimized guide...

...random-walk" of a budding startup, I asked Mike to outline 3 pieces that are essential to the biotech startup puzzle... anywhere. The team should initially consist of 2-3 individuals that are aligned in vision and outcomes...transitioned away from the bench and became all about finding the best product-market fit for this set...Notably, the team must have an upfront conversation about its goals and vision for the company; this will ...section! And read on for more great information about starting a biotech company. Suggested reading The...of Sample6) for taking the time to speak with us about the biotech startup process and work environment...

... cover letter can be found on the Addgene blog.   3. Personal statement brilliance In addition to sending... Letters of recommendation wisdom  Reach out to 2-3 individuals in your professional circle to vouch for...Rose is passionate about outreach among low-income communities to educate parents about resources that can...automated filters of consideration. If you are worried about any eligibility provision, don’t guess. Simply reach...

... they can deplete endogenous protein levels for 1-3 days. There’s also a CRISPR for that! CRISPR interference...most of the other mechanisms described. Fig. 3 dTag mechanism of protein degradation. Adapted from...which can be used with this approach - read more about them on our CRISPRa blog! Fig. 1 CRISPR ... is a lot of overlap with the systems discussed above, just with more elements of control. Tet promoters...to be site-specifically knocked in. This can be labor intensive and time consuming. If your native protein...

...modified from pSC101 with copy numbers ranging from 3-100 per cell, a stabilized promoter buffered against... expression levels returned to the baseline after 3 hours with the stabilized promoter while gene expression...is useful for many metabolic engineering and synthetic biology goals. In metabolic engineering, the expression...or can interfere with the precision needed in metabolic engineering or other synthetic biology circuits...

... Our Segment on these new AAV Vectors Hot Plasmid 3: mCyRFP1 - a new, monomeric, and cyan-excitable red...of our Hot Plasmids podcast series, you'll learn about new red fluorescent proteins, AAV tools for targeting...

...segment on modulating iPSC reprogramming  Hot Plasmid 3: Regulating telomere length with TZAP. Listen to the...of our Hot Plasmids podcast series, you'll learn about new libraries for studying B. subtilis biology, ...

...to the Segement on Ouabain Selection  Hot Plasmid 3: CRISPR base editors with enhanced range and specificity...of our Hot Plasmids podcast series, you'll learn about optogenetics tools for controlling protein activity...

... of conventional gene targeting is lowered to about 2-3 months. But certain models remain more difficult...directly into a zygote and obtain a knockout mouse in about 3 months. Image courtesy of Wenning Qin. Single-stranded...cleavage right before the stop codon!  Figure 3: How to design a C-terminal insertion with Easi-CRISPR... on CRISPR Mouse Models Learn about Homology Directed Repair Learn about Cas9 Ribonucleoprotein (RNP) ... a variety of species, including the standard laboratory mouse. It’s also made targeted insertions relatively... a mouse embryo to create chimeric mice. This laborious process takes ~8-10 months. With CRISPR (B), you...