DAP arrays for multiplex gene editing
Type
Blog Post
...editing at each site. A 75-nt human cysteine tRNA stood out from their initial testing. It outperformed ...
We narrowed to 728 results for: STO
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Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog Post...easily started and easily credited. And nothing stops these one-time favors from becoming starting points... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog Post... saved to your Mendeley library. The library is stored in the cloud, so you can access it from anywhere... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...make lab processes more efficient. Being in the Boston area, we get many opportunities to learn from other... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...their potential binding affinity to major histocompatibility complexes (MHC class I and II). There are... -
Trading Spaces - Visiting Scientist Edition
TypeBlog Post...institute may have a piece of equipment that is custom-built, hard to find, or inaccessible to you for... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog Post... system. TF screens can be designed to identify custom TF programs to acutely initiate disease onset, ... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog Post...publication: References 1. Richardson, Christopher D., et al. "Enhancing homology-directed genome... -
Components of CRISPR/Cas9
TypeBlog Post... to modify the desired genomic locus. Some historical notes CRISPR arrays in bacterial genomes consist... -
Advice for Scientists Starting a Lab
TypeBlog Post...tips for starting a lab. Or share your success stories below. And don't forget to stay tuned for the last... -
Finding and Joining Your Dream Lab
TypeBlog Post... hires are simpler because of their documented history in science. Potential graduate students typically... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...differentiation. Furthermore, TALEN and ZFN require custom nucleases for each gene of interest. CRISPR-Cas9... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...podcast segment As the primary cause for both the stomach flu and food-borne illnesses, noroviruses can infect... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog Post...Consequently it’s best to draft a cover letter that is customized for each internship. Lastly, have your resume... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post...mutate in the genome) and an out-of-frame H2B (histone)-GFP reporter into the genome of mammalian cells... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...be expressed until the base editor corrects early stop codons within T7 RNAP. A phage encoding the deaminase... -
Antibodies 101: ChIP
TypeBlog Post...antibody on crosslinked proteins. In the case of histone modifying proteins, which bind quite strongly to... -
Antibodies 101: Isotypes
TypeBlog Post...antibody-based applications including flow cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post... S., Lee, W., Walsh, N., Iozza, K., Huang, N., Preston, G., Drouin, L. M., Jia, N., Deng, J., Hebben, ... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...mechanisms by which retrograde AAV work are not yet understood, one thing is clear: this application opens a... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...transgene. One such marker is the white gene, which restores eye color to fly lines where the endogenous white... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...deposited at Addgene was used to produce the glioblastoma model for this study (Ref 6)). In addition, ... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...fluorescent proteins. This weak emission hasn’t stopped some scientists from using chemiluminescent proteins... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post...one assay per gene approach that has been the historical standard. Specifically, in our recent publications... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post...Findlay, S. D., Vincent, K. M., Berman, J. R. & Postovit, L.-M. A Digital PCR-Based Method for Efficient... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...these strains should not be used for long term storage. Additional Resources Review of restriction modification... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog Post...people and organizations who helped us reach this milestone. Viral service supporters We would like to thank... -
Viral Vectors 101: Viral Applications
TypeBlog Post...in your gene of interest, for example. Some customization is required, but many viral constructs have ... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...associations: common colds, COVID-19, norovirus (the stomach flu), and many more. Their infectious nature allows... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post...interact with personnel from the industry and brainstorm with each other. The event also featured a seminar...